DOI: 10.1002/gcc.2870130404. Loss of heterozygosity on chromosome arms 5q, IIp, IIq, I3q, and I6p in Human Testicular Germ Cell Tumors

Loss of heterozygosity on chromosome arms 5q, IIp, IIq, I3q, and I6p in Human Testicular Germ Cell Tumors

10.1002/gcc.2870130404

Crossref journal-article

Wiley

Genes, Chromosomes and Cancer (311)

Abstract

AbstractTo identify common regions of deletion in human testicular germ cell tumors (TGCTs), we have screened tumors from 33 patients for loss of heterozygosity (LOH) using Southern blot analysis with 39 polymorphic markers covering 21 chromosome arms. Losses in more than 2 tumors and occurring at a frequency of > 10% were found on chromosome arms 5q, 11p, 11q, 13q, and 16p, the highest being on chromosome arm 5q (19%). It is suggested that tumor suppressor genes on 5q among others may be involved in testicular tumorigenesis and that LOH in this region requires further investigation. No losses were found on 12q and 17p despite the fact that the most common cytogenetic abnormality in TGCTs is an i( 12p) and that the TP53 gene on 17p is the most frequently mutated gene in human cancers. The level of allelic imbalance varied considerably from one chromosome region to another (0–80%) and did not generally reflect the pattern of LOH. It tended to be high in overrepresented regions of the genome, 1q, 7p, and 12p. The tumor from one patient had a seminomatous component and a less differentiated component. We provide evidence for a common origin of both components and show that it is likely that this tumor has progressed from the seminoma to the less differentiated histology. © 1995 Wiley‐Liss, Inc.

Bibliography

AlâJehani, R. M. A., Povey, S., Delhanty, J. D. A., & Parrington, J. M. (1995). Loss of heterozygosity on chromosome arms 5q, IIp, IIq, I3q, and I6p in Human Testicular Germ Cell Tumors. Genes, Chromosomes and Cancer, 13(4), 249â256. Portico.

Authors 4

Rajai M. A. Al‐Jehani (first)
Susan Povey (additional)
Joy D. A. Delhanty (additional)
Jennifer M. Parrington (additional)

References 37 Referenced 16

Al‐JehaniRMA WellsD FoxM ParringtonJM(in press)Correlation of loss of heterozygosity with cytogenetic analysis using G‐banding and fluorescence in situ hybridisation in aneuploid cultures from two human testicular germ cell tumours. Cancer Genet Cytogenet.
10.1038/ng0494-363
10.1016/0165-4608(83)90125-5
10.1002/gcc.2870060405
{'key': 'e_1_2_1_6_1', 'first-page': '5696', 'article-title': 'Chromosomal changes in human primary testicular nonseminomatous germ cell tumours', 'volume': '49', 'author': 'Castedo SMMJ', 'year': '1989', 'journal-title': 'Cancer Res'} / Cancer Res / Chromosomal changes in human primary testicular nonseminomatous germ cell tumours by Castedo SMMJ (1989)
{'key': 'e_1_2_1_7_1', 'first-page': '439', 'article-title': 'Cytogenetic analysis of ten human seminomas', 'volume': '49', 'author': 'Castedo SMMJ', 'year': '1989', 'journal-title': 'Cancer Res'} / Cancer Res / Cytogenetic analysis of ten human seminomas by Castedo SMMJ (1989)
10.1038/305779a0
10.1016/S0140-6736(81)91625-1
10.1016/0165-4608(90)90115-Q
{'key': 'e_1_2_1_11_1', 'first-page': '543', 'article-title': 'Isochromosome 12p in non‐seminoma cell lines: Karyologic amplification of c‐ki‐ras\n 2, without point mutational activation', 'volume': '5', 'author': 'Dmitrovsky E', 'year': '1990', 'journal-title': 'Oncogene'} / Oncogene / Isochromosome 12p in non‐seminoma cell lines: Karyologic amplification of c‐ki‐ras 2, without point mutational activation by Dmitrovsky E (1990)
10.1038/bjc.1992.51
10.1016/0165-4608(89)90154-4
10.1002/gcc.2870060205
10.1038/332278a0
10.1002/gcc.2870090302
10.1016/0888-7543(89)90097-9
10.1002/gcc.2870070206
{'key': 'e_1_2_1_19_1', 'volume-title': 'Molecular Cloning: A Laboratory Manual', 'author': 'Maniatis T', 'year': '1982'} / Molecular Cloning: A Laboratory Manual by Maniatis T (1982)
10.1002/gcc.2870030409
10.1073/pnas.89.22.11006
{'key': 'e_1_2_1_22_1', 'first-page': '2245', 'article-title': 'Allelic loss and somatic differentiation in human male germ cell tumours', 'volume': '9', 'author': 'Murty VWS', 'year': '1994', 'journal-title': 'Oncogene'} / Oncogene / Allelic loss and somatic differentiation in human male germ cell tumours by Murty VWS (1994)
{'key': 'e_1_2_1_23_1', 'first-page': '3227', 'article-title': 'Frequent allelic deletions and loss of expression characterize the DCC gene in male germ cell tumours', 'volume': '9', 'author': 'Murty VWS', 'year': '1994', 'journal-title': 'Oncogene'} / Oncogene / Frequent allelic deletions and loss of expression characterize the DCC gene in male germ cell tumours by Murty VWS (1994)
{'key': 'e_1_2_1_24_1', 'first-page': '3983', 'article-title': 'Replication error‐type genetic instability at 1q42‐q43 in human male germ cell tumours', 'volume': '54', 'author': 'Murty VWS', 'year': '1994', 'journal-title': 'Cancer Res'} / Cancer Res / Replication error‐type genetic instability at 1q42‐q43 in human male germ cell tumours by Murty VWS (1994)
10.1007/BF00284484
10.1016/0165-4608(94)90158-9
10.1016/0165-4608(90)90209-S
{'key': 'e_1_2_1_28_1', 'first-page': '5853', 'article-title': 'Microsatellite instability is associated with tumours that characterize the hereditary non‐polyposis colorectal carcinoma syndrome', 'volume': '53', 'author': 'Peltomaki P', 'year': '1993', 'journal-title': 'Cancer Res'} / Cancer Res / Microsatellite instability is associated with tumours that characterize the hereditary non‐polyposis colorectal carcinoma syndrome by Peltomaki P (1993)
{'key': 'e_1_2_1_29_1', 'first-page': '3574', 'article-title': 'Mutations of the p53 gene do not occur in testis cancer', 'volume': '53', 'author': 'Peng H‐Q', 'year': '1993', 'journal-title': 'Cancer Res'} / Cancer Res / Mutations of the p53 gene do not occur in testis cancer by Peng H‐Q (1993)
10.1159/000132843
10.1002/gcc.2870050104
10.1111/j.1365-2559.1991.tb01502.x
10.1016/0165-4608(94)90082-5
10.1016/S0300-9084(86)80045-1
{'key': 'e_1_2_1_35_1', 'first-page': '4192', 'article-title': 'Amplification and enhanced expression of the c‐Ki‐ras2 protooncogene in human embryonal carcinomas', 'volume': '47', 'author': 'Wang L‐C', 'year': '1987', 'journal-title': 'Cancer Res'} / Cancer Res / Amplification and enhanced expression of the c‐Ki‐ras2 protooncogene in human embryonal carcinomas by Wang L‐C (1987)
{'key': 'e_1_2_1_36_1', 'volume-title': 'Cancer Incidence in Five Continents', 'author': 'Waterhouse JAH', 'year': '1982'} / Cancer Incidence in Five Continents by Waterhouse JAH (1982)
10.1016/S0022-5347(17)35639-2 / J Urol / P53 gene mutations in Chinese human testicular seminoma by Wei YD (1993)
10.1016/0165-4608(82)90077-2

Dates

Type	When
Created	18 years, 6 months ago (Feb. 21, 2007, 7:10 p.m.)
Deposited	1 year, 10 months ago (Oct. 26, 2023, 10:51 a.m.)
Indexed	1 year, 10 months ago (Oct. 27, 2023, 9:10 a.m.)
Issued	30 years ago (Aug. 1, 1995)
Published	30 years ago (Aug. 1, 1995)
Published Online	19 years, 1 month ago (July 14, 2006)
Published Print	30 years ago (Aug. 1, 1995)

Funders 0

None

BibTeX

@article{Al_Jehani_1995, title={Loss of heterozygosity on chromosome arms 5q, IIp, IIq, I3q, and I6p in Human Testicular Germ Cell Tumors}, volume={13}, ISSN={1098-2264}, url={http://dx.doi.org/10.1002/gcc.2870130404}, DOI={10.1002/gcc.2870130404}, number={4}, journal={Genes, Chromosomes and Cancer}, publisher={Wiley}, author={Al‐Jehani, Rajai M. A. and Povey, Susan and Delhanty, Joy D. A. and Parrington, Jennifer M.}, year={1995}, month=aug, pages={249–256} }

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