DOI: 10.1002/gcc.2870040311. Cytogenetic analysis by chromosome painting using dop‐pcr amplified flow‐sorted chromosomes

Cytogenetic analysis by chromosome painting using dop‐pcr amplified flow‐sorted chromosomes

10.1002/gcc.2870040311

Crossref journal-article

Wiley

Genes, Chromosomes and Cancer (311)

Abstract

AbstractA novel polymerase chain reaction (PCR) technique has been combined with chromosome flow sorting to characterise two lymphoblastoid cell lines and one medullary thyroid carcinoma cell line carrying translocations close to the locus for multiple endocrine neoplasia type 2A (MEN 2A). Five hundred copies of the derivative chromosome(s) were flow sorted from each cell line and amplified by degenerate oligonucleotide‐primed–polymerase chain reaction (DOP‐PCR). This generated pools of DNA sequences corresponding to the abnormal chromosomes, which were then used as probes in fluorescence in situ hybridisation (FISH) experiments on normal metaphase cells. The resultant chromosome paints revealed the portions of the normal chromosomes related to those involved in the translocations. By this technique, translocation breakpoints in bands p15, q11.2, and q21 of chromosome 10 were defined in the above cell lines, in two cases refining previous cytogenetic data. This study shows that flow sorting of aberrant chromosomes and chromosome painting can be used as a rapid aid to cytogenetic analysis, particularly in cases of difficult karyotypes, such as tumours. Furthermore, the DOP‐PCR technique described here will have applications to other areas of genome analysis, such as cloning of new markers; its design will allow a general and representative amplification to occur from any starting DNA in any species.

Bibliography

Telenius, HÃ¥k., Ponder, B. A. J., Tunnacliffe, A., Pelmear, A. H., Carter, N. P., FergusonâSmith, M. A., Behmel, A., NordenskjÃ¶ld, M., & Pfragner, R. (1992). Cytogenetic analysis by chromosome painting using dopâpcr amplified flowâsorted chromosomes. Genes, Chromosomes and Cancer, 4(3), 257â263. Portico.

Authors 9

HÅKan Telenius (first)
Bruce A. J. Ponder (additional)
Alan Tunnacliffe (additional)
Adèle H. Pelmear (additional)
Nigel P. Carter (additional)
Malcolm A. Ferguson‐Smith (additional)
Annemarie Behmel (additional)
Magnus Nordenskjöld (additional)
Roswitha Pfragner (additional)

References 17 Referenced 416

10.1016/0888-7543(91)90374-N
10.1002/cyto.990110123
10.1007/BF00294840
{'key': 'e_1_2_1_5_1', 'first-page': '318', 'volume-title': 'Cancer Cytogenetics', 'author': 'Heim S', 'year': '1987'} / Cancer Cytogenetics by Heim S (1987)
10.1016/0092-8674(88)90159-6
10.1126/science.6587566
10.1073/pnas.85.24.9664
10.1038/328527a0
10.1073/pnas.86.17.6686
{'key': 'e_1_2_1_11_1', 'first-page': '4160', 'article-title': 'Establishment and characterisation of continuous cell line MTC‐SK derived from a human medullary thyroid carcinoma', 'volume': '50', 'author': 'Pfragner R', 'year': '1990', 'journal-title': 'Cancer Res'} / Cancer Res / Establishment and characterisation of continuous cell line MTC‐SK derived from a human medullary thyroid carcinoma by Pfragner R (1990)
10.1073/pnas.85.23.9138
10.1073/pnas.83.9.2934
{'key': 'e_1_2_1_14_1', 'volume-title': 'Human Cytogenetics: A Practical Approach', 'author': 'Rooney DE', 'year': '1986'} / Human Cytogenetics: A Practical Approach by Rooney DE (1986)
10.1126/science.2999980
10.1038/328528a0
TeleniusH CarterNP BebbC NordenskjöldM PonderBAJ TunnacliffeA(1992) Degenerate Oligonucleotide‐Primed PCR (DOP‐PCR): general amplification of target DNA by a single degenerate primer.Genomics in press. (10.1016/0888-7543(92)90147-K)
10.1073/pnas.78.12.7727

Dates

Type	When
Created	18 years, 6 months ago (Feb. 21, 2007, 6:12 p.m.)
Deposited	1 year, 10 months ago (Oct. 23, 2023, 5:02 a.m.)
Indexed	1 hour, 28 minutes ago (Aug. 28, 2025, 7:59 a.m.)
Issued	33 years, 4 months ago (April 1, 1992)
Published	33 years, 4 months ago (April 1, 1992)
Published Online	19 years, 1 month ago (July 14, 2006)
Published Print	33 years, 4 months ago (April 1, 1992)

Funders 0

None

BibTeX

@article{Telenius_1992, title={Cytogenetic analysis by chromosome painting using dop‐pcr amplified flow‐sorted chromosomes}, volume={4}, ISSN={1098-2264}, url={http://dx.doi.org/10.1002/gcc.2870040311}, DOI={10.1002/gcc.2870040311}, number={3}, journal={Genes, Chromosomes and Cancer}, publisher={Wiley}, author={Telenius, HÅKan and Ponder, Bruce A. J. and Tunnacliffe, Alan and Pelmear, Adèle H. and Carter, Nigel P. and Ferguson‐Smith, Malcolm A. and Behmel, Annemarie and Nordenskjöld, Magnus and Pfragner, Roswitha}, year={1992}, month=apr, pages={257–263} }

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