DOI: 10.1002/gcc.20310. Amplification of <i>CDK4</i> and <i>MDM2</i> in malignant melanoma

Amplification of CDK4 and MDM2 in malignant melanoma

10.1002/gcc.20310

Crossref journal-article

Wiley

Genes, Chromosomes and Cancer (311)

Abstract

AbstractAmplification of the 12q13–15 region is a common event in several human tumors including liposarcomas, gliomas, and osteosarcomas. We have demonstrated high‐level amplification of 12q14 in a subset of uncultured malignant melanomas (3 of 53). High‐resolution mapping of the amplicon using quantitative PCR revealed a bipartite amplicon consisting of a primary 50‐kb amplicon centered on CDK4 and a secondary amplicon centered on MDM2, without amplification of the intervening 11 Mb of genomic DNA. Analysis of mRNA and protein levels in melanomas with 12q14 amplification demonstrated overexpression of target genes CDK4 and MDM2 without loss of CDKN2A‐P16 (P16INK4A) or CDKN2A‐P14ARF (P14ARF) expression, important regulators of the RB1 and TP53 pathways, which are commonly lost or mutated in melanoma. These results suggest that coamplification of CDK4 and MDM2 may substitute for loss of P16INK4A and P14ARF function in a subset of melanomas. © 2006 Wiley‐Liss, Inc.

Bibliography

Muthusamy, V., Hobbs, C., Nogueira, C., CordonâCardo, C., McKee, P. H., Chin, L., & Bosenberg, M. W. (2006). Amplification of CDK4 and MDM2 in malignant melanoma. Genes, Chromosomes and Cancer, 45(5), 447â454. Portico.

Authors 7

Viswanathan Muthusamy (first)
Cara Hobbs (additional)
Cristina Nogueira (additional)
Carlos Cordon‐Cardo (additional)
Phillip H. McKee (additional)
Lynda Chin (additional)
Marcus W. Bosenberg (additional)

References 22 Referenced 123

10.1128/MCB.21.6.2144-2153.2001
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{'key': 'e_1_2_6_4_1', 'first-page': '1968', 'article-title': 'Gene amplifications characterize acral melanoma and permit the detection of occult tumor cells in the surrounding skin', 'volume': '60', 'author': 'Bastian BC', 'year': '2000', 'journal-title': 'Cancer Res'} / Cancer Res / Gene amplifications characterize acral melanoma and permit the detection of occult tumor cells in the surrounding skin by Bastian BC (2000)
10.1016/S0002-9440(10)63536-5
10.1002/(SICI)1098-2264(199612)17:4<254::AID-GCC7>3.0.CO;2-2
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{'key': 'e_1_2_6_15_1', 'first-page': '5535', 'article-title': 'Coamplification of the CDK4 gene with MDM2 and GLI in human sarcomas', 'volume': '53', 'author': 'Khatib ZA', 'year': '1993', 'journal-title': 'Cancer Res'} / Cancer Res / Coamplification of the CDK4 gene with MDM2 and GLI in human sarcomas by Khatib ZA (1993)
{'key': 'e_1_2_6_16_1', 'first-page': '7642', 'article-title': 'HDM2 protein overexpression, but not gene amplification, is related to tumorigenesis of cutaneous melanoma', 'volume': '61', 'author': 'Polsky D', 'year': '2001', 'journal-title': 'Cancer Res'} / Cancer Res / HDM2 protein overexpression, but not gene amplification, is related to tumorigenesis of cutaneous melanoma by Polsky D (2001)
{'key': 'e_1_2_6_17_1', 'first-page': '5141', 'article-title': 'Refined mapping of 12q13–q15 amplicons in human malignant gliomas suggests CDK4/SAS and MDM2 as independent amplification targets', 'volume': '56', 'author': 'Reifenberger G', 'year': '1996', 'journal-title': 'Cancer Res'} / Cancer Res / Refined mapping of 12q13–q15 amplicons in human malignant gliomas suggests CDK4/SAS and MDM2 as independent amplification targets by Reifenberger G (1996)
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10.1002/gcc.20122

Dates

Type	When
Created	19 years, 7 months ago (Jan. 17, 2006, 3:47 p.m.)
Deposited	1 year, 10 months ago (Oct. 16, 2023, 8:43 a.m.)
Indexed	2 months ago (June 27, 2025, 12:22 p.m.)
Issued	19 years, 7 months ago (Jan. 17, 2006)
Published	19 years, 7 months ago (Jan. 17, 2006)
Published Online	19 years, 7 months ago (Jan. 17, 2006)
Published Print	19 years, 4 months ago (May 1, 2006)

Funders 0

None

BibTeX

@article{Muthusamy_2006, title={Amplification of CDK4 and MDM2 in malignant melanoma}, volume={45}, ISSN={1098-2264}, url={http://dx.doi.org/10.1002/gcc.20310}, DOI={10.1002/gcc.20310}, number={5}, journal={Genes, Chromosomes and Cancer}, publisher={Wiley}, author={Muthusamy, Viswanathan and Hobbs, Cara and Nogueira, Cristina and Cordon‐Cardo, Carlos and McKee, Phillip H. and Chin, Lynda and Bosenberg, Marcus W.}, year={2006}, month=jan, pages={447–454} }

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