DOI: 10.1002/elps.200405998. Ultrafiltration for proteomic sample preparation

Ultrafiltration for proteomic sample preparation

10.1002/elps.200405998

Crossref journal-article

Wiley

ELECTROPHORESIS (311)

Abstract

AbstractProteome analysis represents significant challenges to the existing sample preparation techniques. Traditional methods, such as two‐dimensional electrophoresis, typically separate high‐molecular‐weight proteins while discarding low‐molecular‐weight species. This approach is well justified considering the complexity of any proteome. However, it is desirable to extract the maximum amount of information from each sample to investigate the entire range of biomolecules. We have demonstrated that ultrafiltration not only improves two‐dimensional electrophoresis (2‐DE) resolution of the protein fraction but also yields the low‐molecular‐weight fraction amenable for further analysis by high‐resolution mass spectrometry. This approach was successfully adapted to the variety of biological samples including cell and tissue lysates and serum. Therefore, ultrafiltration offers an alternative sample preparation technique that enables more thorough analysis of a proteome.

Bibliography

Chernokalskaya, E., Gutierrez, S., Pitt, A. M., & Leonard, J. T. (2004). Ultrafiltration for proteomic sample preparation. ELECTROPHORESIS, 25(15), 2461â2468. Portico.

Authors 4

Elena Chernokalskaya (first)
Sara Gutierrez (additional)
Aldo M. Pitt (additional)
Jack T. Leonard (additional)

References 27 Referenced 41

10.1016/S1044-0305(98)00117-2
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10.1097/00005344-198706107-00008

Dates

Type	When
Created	21 years, 1 month ago (Aug. 2, 2004, 8:44 a.m.)
Deposited	1 year, 11 months ago (Sept. 11, 2023, 9:08 p.m.)
Indexed	11 months, 3 weeks ago (Sept. 15, 2024, 9:09 a.m.)
Issued	21 years, 1 month ago (Aug. 1, 2004)
Published	21 years, 1 month ago (Aug. 1, 2004)
Published Online	21 years, 1 month ago (Aug. 2, 2004)
Published Print	21 years, 1 month ago (Aug. 1, 2004)

Funders 0

None

BibTeX

@article{Chernokalskaya_2004, title={Ultrafiltration for proteomic sample preparation}, volume={25}, ISSN={1522-2683}, url={http://dx.doi.org/10.1002/elps.200405998}, DOI={10.1002/elps.200405998}, number={15}, journal={ELECTROPHORESIS}, publisher={Wiley}, author={Chernokalskaya, Elena and Gutierrez, Sara and Pitt, Aldo M. and Leonard, Jack T.}, year={2004}, month=aug, pages={2461–2468} }

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