DOI: 10.1002/elps.1150170516. Sequence patterns produced by incomplete enzymatic digestion or one‐step Edman degradation of peptide mixtures as probes for protein database searches

Sequence patterns produced by incomplete enzymatic digestion or one‐step Edman degradation of peptide mixtures as probes for protein database searches

10.1002/elps.1150170516

Crossref journal-article

Wiley

ELECTROPHORESIS (311)

Abstract

AbstractMass spectrometric peptide mapping of proteins isolated by polyacrylamide gel electrophoresis is a rapid method for identifying proteins in sequence databases. A majority of tryptic peptide maps were found to contain pairs of peptide ion peaks separated by the molecular weight of the lysyl or arginyl residue. These peaks originate from amino acid sequence patterns such as Lys‐Lys where trypsin has cleaved C‐terminals to either one of the lysines. The peptide mass and the pattern define an N‐ or C‐terminal sequence tag. Searching sequence databases by such a sequence tag results in only a moderate number of matches and significantly reduces the number of database matches when used in combination with a peptide mass map. Two N‐ or C‐terminal sequence tags alone unambiguously identify a protein in most cases. The technique discussed here is simple, does not require additional measurments, and increases the percentage of protein samples that can be identified by their mass maps alone. N‐Terminal peptide sequence tags for database searching can also be generated by manual one‐step Edman degradation of the unseparated peptide mixture.

Bibliography

Jensen, O. N., Vorm, O., & Mann, M. (1996). Sequence patterns produced by incomplete enzymatic digestion or oneâstep Edman degradation of peptide mixtures as probes for protein database searches. ELECTROPHORESIS, 17(5), 938â944. Portico.

Authors 3

Ole N. Jensen (first)
Ole Vorm (additional)
Matthias Mann (additional)

References 21 Referenced 44

10.1002/elps.11501601299
Henzel W. J. Stults J. T. Watanabe C. Abstract of the 3rd Protein Society Symposium Seattle1989.
10.1073/pnas.90.11.5011
10.1006/bbrc.1993.2009
10.1002/bms.1200220605
10.1006/abio.1993.1514
10.1016/0960-9822(93)90195-T
10.1002/elps.1150150159
10.1002/elps.1150160171
10.1073/pnas.92.11.5072
10.1126/science.270.5235.369
10.1016/1044-0305(94)80013-8
10.1021/ac00091a044
10.1021/ac9509519
10.1038/379466a0
10.1021/ac00096a002
10.1021/ac950914h
{'key': 'e_1_2_1_19_2', 'volume-title': 'Practical Guide to Protein and Peptide Purification for Microsequencing', 'author': 'Matsudaira P. A.', 'year': '1993'} / Practical Guide to Protein and Peptide Purification for Microsequencing by Matsudaira P. A. (1993)
Vorm O. Roepstorff P. J. Mass Spectrom.1996 in press.
10.1016/B978-0-12-194710-1.50008-4
10.1002/rcm.1290080916

Dates

Type	When
Created	20 years, 2 months ago (May 28, 2005, 11:16 a.m.)
Deposited	1 year, 9 months ago (Oct. 27, 2023, 1:54 a.m.)
Indexed	1 year, 6 months ago (Jan. 27, 2024, 5:18 a.m.)
Issued	29 years, 7 months ago (Jan. 1, 1996)
Published	29 years, 7 months ago (Jan. 1, 1996)
Published Online	20 years, 4 months ago (April 14, 2005)
Published Print	29 years, 7 months ago (Jan. 1, 1996)

Funders 0

None

BibTeX

@article{Jensen_1996, title={Sequence patterns produced by incomplete enzymatic digestion or one‐step Edman degradation of peptide mixtures as probes for protein database searches}, volume={17}, ISSN={1522-2683}, url={http://dx.doi.org/10.1002/elps.1150170516}, DOI={10.1002/elps.1150170516}, number={5}, journal={ELECTROPHORESIS}, publisher={Wiley}, author={Jensen, Ole N. and Vorm, Ole and Mann, Matthias}, year={1996}, month=jan, pages={938–944} }

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