DOI: 10.1002/elps.1150170333. Two‐dimensional electrophoretic patterns of acute‐phase human serum proteins in the course of bacterial and viral diseases

Two‐dimensional electrophoretic patterns of acute‐phase human serum proteins in the course of bacterial and viral diseases

10.1002/elps.1150170333

Crossref journal-article

Wiley

ELECTROPHORESIS (311)

Abstract

AbstractAcute‐phase serum proteins were analyzed by two‐dimensional electrophoresis with isoelectric focusing in 3‐10 immobilized pH gradients. Most spots were identified by reference to the plasma map in the SWISS‐2DPAGE database. Serum amyloid A protein spots were identified by immunoblotting with specific antiserum and by matching determined with predicted values of electrophoretic parameters. Changes in the concentrations of α1‐antitrypsin, leucinerich glycoprotein, haptoglobin, serum retinol‐binding protein and transthyretin were quantitated by densitometry of silver‐stained gels. Electrophoretic patterns from 18 patients with bacterial diseases and 16 patients with viral diseases were compared. The incidence of serum amyloid A protein spots was 18/18 in bacterial diseases and 6/16 in viral diseases. As for the other reactants studied, variations were simultaneous in bacterial disease and tended to be staggered in viral diseases.

Bibliography

Bini, L., Magi, B., Marzocchi, B., Cellesi, C., Berti, B., Raggiaschi, R., Rossolini, A., & Pallini, V. (1996). Twoâdimensional electrophoretic patterns of acuteâphase human serum proteins in the course of bacterial and viral diseases. ELECTROPHORESIS, 17(3), 612â616. Portico.

Authors 8

Luca Bini (first)
Barbara Magi (additional)
Barbara Marzocchi (additional)
Carla Cellesi (additional)
Brunilde Berti (additional)
Roberto Raggiaschi (additional)
Aldo Rossolini (additional)
Vitaliano Pallini (additional)

References 16 Referenced 58

{'key': 'e_1_2_1_2_2', 'volume-title': 'Marker Proteins in Inflammation', 'author': 'Allen R. C.', 'year': '1982'} / Marker Proteins in Inflammation by Allen R. C. (1982)
10.1016/S0065-2776(08)60379-X
10.1016/0076-6879(88)63037-0
{'key': 'e_1_2_1_5_2', 'first-page': '331', 'volume': '66', 'author': 'Pluschke G.', 'year': '1986', 'journal-title': 'Clin. Exp. Immunol.'} / Clin. Exp. Immunol. by Pluschke G. (1986)
10.1002/elps.1150101110
10.1002/elps.1150120703
10.1016/0003-2697(88)90209-6
10.1002/elps.11501301161
10.1002/elps.11501401209
10.1002/elps.1150121006
10.1002/elps.11501401183
10.1002/elps.11501601190
10.1002/elps.11501401163
10.1002/elps.11501401185
10.1016/0021-9150(93)90155-N
10.1073/pnas.76.9.4350 / Proc. Natl. Acad. Sci. USA by Towbin H. (1997)

Dates

Type	When
Created	20 years, 3 months ago (May 28, 2005, 11:05 a.m.)
Deposited	1 year, 10 months ago (Oct. 26, 2023, 9:48 p.m.)
Indexed	1 year, 3 months ago (May 17, 2024, 12:17 p.m.)
Issued	29 years, 8 months ago (Jan. 1, 1996)
Published	29 years, 8 months ago (Jan. 1, 1996)
Published Online	20 years, 4 months ago (April 14, 2005)
Published Print	29 years, 8 months ago (Jan. 1, 1996)

Funders 0

None

BibTeX

@article{Bini_1996, title={Two‐dimensional electrophoretic patterns of acute‐phase human serum proteins in the course of bacterial and viral diseases}, volume={17}, ISSN={1522-2683}, url={http://dx.doi.org/10.1002/elps.1150170333}, DOI={10.1002/elps.1150170333}, number={3}, journal={ELECTROPHORESIS}, publisher={Wiley}, author={Bini, Luca and Magi, Barbara and Marzocchi, Barbara and Cellesi, Carla and Berti, Brunilde and Raggiaschi, Roberto and Rossolini, Aldo and Pallini, Vitaliano}, year={1996}, month=jan, pages={612–616} }

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