DOI: 10.1002/elps.1150170329. Characterisation of proteins from two‐dimensional electrophoresis gels by matrix‐assisted laser desorption mass spectrometry and amino acid compositional analysis

Characterisation of proteins from two‐dimensional electrophoresis gels by matrix‐assisted laser desorption mass spectrometry and amino acid compositional analysis

10.1002/elps.1150170329

Crossref journal-article

Wiley

ELECTROPHORESIS (311)

Abstract

AbstractAmino acid compositional analysis and peptide mass fingerprinting by matrix assisted laser desorption mass spectrometry have been used to characterise proteins obtained from two‐dimensional electrophoresis (2‐DE) separations of human cardiac proteins. A group of twelve protein spots was selected for analysis. The identities of eight of the proteins had been determined by conventional protein characterisation methods, two were unknown proteins and two had putative identities from protein database spot comparison. Amino acid analysis and peptide mass fingerprinting gave corresponding identities for seven of the twelve proteins, which also agreed with our initial identifications. Three proteins which had been identified previously were not confirmed in this study and putative identities were obtained for the two unknown proteins. The advantages, problems and use of amino acid analysis and peptide mass fingerprinting for the analysis of proteins from 2‐DE are discussed. The data highlight the need to use orthogonal techniques for the unequivocal identification of proteins from 2‐DE gels.

Bibliography

Wheeler, C. H., Berry, S. L., Wilkins, M. R., Corbett, J. M., Ou, K., Gooley, A. A., HumpheryâSmith, I., Williams, K. L., & Dunn, M. J. (1996). Characterisation of proteins from twoâdimensional electrophoresis gels by matrixâassisted laser desorption mass spectrometry and amino acid compositional analysis. ELECTROPHORESIS, 17(3), 580â587. Portico.

Authors 9

Colin H. Wheeler (first)
Sophie L. Berry (additional)
Marc R. Wilkins (additional)
Joseph M. Corbett (additional)
Keli Ou (additional)
Andrew A. Gooley (additional)
Ian Humphery‐Smith (additional)
Keith L. Williams (additional)
Michael J. Dunn (additional)

References 29 Referenced 56

{'key': 'e_1_2_1_2_2', 'first-page': '1', 'volume': '3', 'author': 'Celis J. E.', 'year': '1989', 'journal-title': 'Adv. Electrophorsis'} / Adv. Electrophorsis by Celis J. E. (1989)
10.1002/elps.11501501208
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10.1002/elps.11501601192
10.1002/elps.11501601190
10.1002/elps.11501601138
10.1080/02648725.1996.10647923
10.1038/nbt0196-61
10.1006/abio.1994.1371
{'key': 'e_1_2_1_12_2', 'first-page': '1971', 'volume': '16', 'author': 'Patterson S. D.', 'year': '1995', 'journal-title': 'Electrophoresis'} / Electrophoresis by Patterson S. D. (1995)
10.1073/pnas.90.11.5011
10.1006/abio.1993.1514
{'key': 'e_1_2_1_15_2', 'first-page': '115', 'volume': '7', 'author': 'Cottrell J. S.', 'year': '1994', 'journal-title': 'Peptide Res.'} / Peptide Res. by Cottrell J. S. (1994)
10.1002/elps.1150091208
10.1007/BF01024960
10.1006/abio.1994.1474
10.1002/elps.1150160171
10.1002/elps.11501601186
10.1002/elps.1150160151
10.1002/elps.11501301154
10.1016/0960-9822(93)90195-T
10.1016/0021-9673(91)85012-5
Ou K. Wilkins M. R. Yan J. X. Gooley A. A. Fung Y. Scheumack D. Williams K. L. J. Chromatogr.1996 in press.
10.1002/elps.11501401185
10.1016/0968-0004(94)90153-8
10.1073/pnas.80.12.3734
{'key': 'e_1_2_1_29_2', 'first-page': 'S188', 'volume': '14', 'author': 'Wheeler C. H.', 'year': '1995', 'journal-title': 'J. Heart Lung Transplant.'} / J. Heart Lung Transplant. by Wheeler C. H. (1995)
10.1016/S0022-2836(05)80360-2

Dates

Type	When
Created	20 years, 3 months ago (May 28, 2005, 11:05 a.m.)
Deposited	1 year, 10 months ago (Oct. 26, 2023, 9:49 p.m.)
Indexed	1 year, 3 months ago (May 13, 2024, 11:15 p.m.)
Issued	29 years, 8 months ago (Jan. 1, 1996)
Published	29 years, 8 months ago (Jan. 1, 1996)
Published Online	20 years, 4 months ago (April 14, 2005)
Published Print	29 years, 8 months ago (Jan. 1, 1996)

Funders 0

None

BibTeX

@article{Wheeler_1996, title={Characterisation of proteins from two‐dimensional electrophoresis gels by matrix‐assisted laser desorption mass spectrometry and amino acid compositional analysis}, volume={17}, ISSN={1522-2683}, url={http://dx.doi.org/10.1002/elps.1150170329}, DOI={10.1002/elps.1150170329}, number={3}, journal={ELECTROPHORESIS}, publisher={Wiley}, author={Wheeler, Colin H. and Berry, Sophie L. and Wilkins, Marc R. and Corbett, Joseph M. and Ou, Keli and Gooley, Andrew A. and Humphery‐Smith, Ian and Williams, Keith L. and Dunn, Michael J.}, year={1996}, month=jan, pages={580–587} }

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