DOI: 10.1002/elps.11501601315. Feasibility study of a drug immunoassay based on micellar electrokinetic capillary chromatography with laser induced fluorescence detection: Determination of theophylline in serum

Feasibility study of a drug immunoassay based on micellar electrokinetic capillary chromatography with laser induced fluorescence detection: Determination of theophylline in serum

10.1002/elps.11501601315

Crossref journal-article

Wiley

ELECTROPHORESIS (311)

Abstract

AbstractThis paper presents principle and first results of a novel competitive binding immunoassay for monitoring of theophylline in human serum. The assay is based upon short time incubation of a mixture of antiserum, containing the antibody raised against theophylline, fluorescein labelled theophylline (tracer) and serum prior to injection of a few nanoliter of this mixture onto a fusedsilica capillary for subsequent separation and analysis of free tracer and the antibody‐tracer‐complex by micellar electrokinetic capillary chromatography with laser induced fluorescence detection. Quantitation based upon multi‐level calibration using the height of the peak produced by the free tracer is shown to provide theophylline serum levels which are in agreement with those obtained by a commercial fluorescence polarization immunoassay and with those determined by micellar elektrokinetic capillary chromatography with direct serum injection and on‐column UV absorption detection.

Bibliography

Steinmann, L., Caslavska, J., & Thormann, W. (1995). Feasibility study of a drug immunoassay based on micellar electrokinetic capillary chromatography with laser induced fluorescence detection: Determination of theophylline in serum. ELECTROPHORESIS, 16(1), 1912â1916. Portico.

Authors 3

Lone Steinmann (first)
Jitka Caslavska (additional)
Wolfgang Thormann (additional)

References 22 Referenced 41

{'key': 'e_1_2_1_2_2', 'volume-title': 'A Textbook for the Clinical Application of TDM', 'author': 'Taylor W. J.', 'year': '1986'} / A Textbook for the Clinical Application of TDM by Taylor W. J. (1986)
10.1002/elps.1150150103
10.1016/0165-9936(89)85022-8
10.1002/elps.1150110905
10.1002/elps.1150150109
10.1002/elps.11501501197
10.1097/00007691-199308000-00009
10.1097/00007691-199410000-00007
{'key': 'e_1_2_1_10_2', 'first-page': '208', 'volume': '1', 'author': 'Zhang C.‐X.', 'year': '1994', 'journal-title': 'J. Capillary Electrophoresis'} / J. Capillary Electrophoresis by Zhang C.‐X. (1994)
10.1016/S0021-9673(01)95371-3
10.1021/ac00069a035
10.1021/ac00073a004
10.1016/0021-9673(93)80659-V
10.1021/ac00094a027
10.1016/0021-9673(94)85138-7
10.1021/ac00101a020
10.1002/elps.1150150104
10.1016/0021-9673(94)85139-5
10.1016/0021-9673(94)85158-1
10.1093/clinchem/40.9.1819
10.1021/ac00099a019
{'key': 'e_1_2_1_23_2', 'volume-title': 'TDxFLx Assay Manual'} / TDxFLx Assay Manual

Dates

Type	When
Created	20 years, 3 months ago (May 28, 2005, 10:29 a.m.)
Deposited	1 year, 10 months ago (Oct. 25, 2023, 10:19 a.m.)
Indexed	1 year, 10 months ago (Oct. 28, 2023, 6:49 p.m.)
Issued	30 years, 8 months ago (Jan. 1, 1995)
Published	30 years, 8 months ago (Jan. 1, 1995)
Published Online	20 years, 4 months ago (April 13, 2005)
Published Print	30 years, 8 months ago (Jan. 1, 1995)

Funders 0

None

BibTeX

@article{Steinmann_1995, title={Feasibility study of a drug immunoassay based on micellar electrokinetic capillary chromatography with laser induced fluorescence detection: Determination of theophylline in serum}, volume={16}, ISSN={1522-2683}, url={http://dx.doi.org/10.1002/elps.11501601315}, DOI={10.1002/elps.11501601315}, number={1}, journal={ELECTROPHORESIS}, publisher={Wiley}, author={Steinmann, Lone and Caslavska, Jitka and Thormann, Wolfgang}, year={1995}, month=jan, pages={1912–1916} }

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