DOI: 10.1002/elps.11501601185. Progress with gene‐product mapping of the Mollicutes: <i>Mycoplasma genitalium</i>

Progress with gene‐product mapping of the Mollicutes: Mycoplasma genitalium

10.1002/elps.11501601185

Crossref journal-article

Wiley

ELECTROPHORESIS (311)

Abstract

AbstractA protein map of the smallest known self‐replicating organism, Mycoplasma genitalium (Class: Mollicutes), revealed a high proportion of acidic proteins. Amino acid composition was used to putatively identify, or provide unique parameters, for 50 gene products separated by two‐dimensional gel electrophoresis. A further 19 proteins were subjected to peptide‐mass fingerprinting using matrix‐assisted laser desorption ionisation‐time of flight (MALDI‐TOF) mass spectrometry and 4 were subjected to N‐terminal Edman degradation. The majority of M. genitalium proteins remain uncharacterised. However, the combined approach of amino acid analysis and peptide‐mass fingerprinting allowed gene products to be linked to homologous genes in a variety of organisms. This has allowed proteins to be identified prior to detection of their respective genes via the M. genitalium sequencing initiative. The principle of ‘hierarchical’ analysis for the mass screening of proteins and the analysis of microbial genomes via their protein complement or ‘proteome’ is detailed. Here, characterisation of gene products depends upon the quickest and most economical technologies being employed initially, so as to determine if a large number of proteins are already present in both homologous and heterologous species databases. Initial screening, which lends itself to automation and robotics, can then be followed by more time and cost intensive procedures, when necessary.

Bibliography

Wasinger, V. C., Cordwell, S. J., CerpaâPoljak, A., Yan, J. X., Gooley, A. A., Wilkins, M. R., Duncan, M. W., Harris, R., Williams, K. L., & HumpheryâSmith, I. (1995). Progress with geneâproduct mapping of the Mollicutes: Mycoplasma genitalium. ELECTROPHORESIS, 16(1), 1090â1094. Portico.

Authors 10

Valerie C. Wasinger (first)
Stuart J. Cordwell (additional)
Anne Cerpa‐Poljak (additional)
Jun X. Yan (additional)
Andrew A. Gooley (additional)
Marc R. Wilkins (additional)
Mark W. Duncan (additional)
Ray Harris (additional)
Keith L. Williams (additional)
Ian Humphery‐Smith (additional)

References 32 Referenced 734

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10.1002/elps.1150160171
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10.1002/elps.1150150197
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10.1016/0960-9822(93)90195-T
10.1006/bbrc.1993.2009
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10.1128/jb.171.12.6455-6467.1989 / J. Bacteriol. by Tully J. G. (1989)
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10.3891/acta.chem.scand.04-0283
{'key': 'e_1_2_1_24_2', 'first-page': '1', 'volume-title': 'Protein Sequencing – A Practical Approach', 'author': 'Findlay J. B. C.', 'year': '1989'} / Protein Sequencing – A Practical Approach by Findlay J. B. C. (1989)
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Dates

Type	When
Created	20 years, 3 months ago (May 28, 2005, 10:29 a.m.)
Deposited	1 year, 10 months ago (Oct. 25, 2023, 10:07 a.m.)
Indexed	2 weeks, 4 days ago (Aug. 12, 2025, 5:36 p.m.)
Issued	30 years, 7 months ago (Jan. 1, 1995)
Published	30 years, 7 months ago (Jan. 1, 1995)
Published Online	20 years, 4 months ago (April 14, 2005)
Published Print	30 years, 7 months ago (Jan. 1, 1995)

Funders 0

None

BibTeX

@article{Wasinger_1995, title={Progress with gene‐product mapping of the Mollicutes: Mycoplasma genitalium}, volume={16}, ISSN={1522-2683}, url={http://dx.doi.org/10.1002/elps.11501601185}, DOI={10.1002/elps.11501601185}, number={1}, journal={ELECTROPHORESIS}, publisher={Wiley}, author={Wasinger, Valerie C. and Cordwell, Stuart J. and Cerpa‐Poljak, Anne and Yan, Jun X. and Gooley, Andrew A. and Wilkins, Marc R. and Duncan, Mark W. and Harris, Ray and Williams, Keith L. and Humphery‐Smith, Ian}, year={1995}, month=jan, pages={1090–1094} }

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