DOI: 10.1002/elps.1150150197. Protein composition of the human heart: The construction of a myocardial two‐dimensional electrophoresis database

Protein composition of the human heart: The construction of a myocardial two‐dimensional electrophoresis database

10.1002/elps.1150150197

Crossref journal-article

Wiley

ELECTROPHORESIS (311)

Abstract

AbstractMolecular changes occurring in myocardial diseases are not well understood. Proteins, as regulatory molecules, should play an important role in the etiology of these diseases. The method of two‐dimensional electrophoresis (2‐DE) allows the analysis of some thousand proteins with one experiment. An important prerequisite for this kind of investigation is the possibility of identifying the proteins separated by 2‐DE. We resolved 3239 proteins of the human myocardium and tried to identify 33 proteins by amino acid analysis and microsequencing. Twenty proteins were identified by search for the proteinchemical data obtained in the Martinsried Institute Protein Sequence Database. Comparisons of 2‐DE patterns of different size, which were obtained in different laboratories, were performed with the result that proteins identified on a 2‐DE map of one laboratory can be assigned to spots of 2‐DE maps produced by another laboratory. Our results show the usefulness of a myocardial 2‐DE database; they can be used in different laboratories and make it possible to generate a collection of important human myocardial proteins in a 2‐DE database for comparative studies worldwide.

Bibliography

Jungblut, P., Otto, A., ZeindlâEberhart, E., PleiÃner, K., Knecht, M., RegitzâZagrosek, V., Fleck, E., & WittmannâLiebold, B. (1994). Protein composition of the human heart: The construction of a myocardial twoâdimensional electrophoresis database. ELECTROPHORESIS, 15(1), 685â707. Portico.

Authors 8

Peter Jungblut (first)
Albrecht Otto (additional)
Evelyn Zeindl‐Eberhart (additional)
Klaus‐P. Pleißner (additional)
Mathias Knecht (additional)
Vera Regitz‐Zagrosek (additional)
Eckart Fleck (additional)
Brigitte Wittmann‐Liebold (additional)

References 40 Referenced 88

10.1073/pnas.89.12.5251
10.1002/clc.4960090506
10.1161/01.RES.59.3.297
10.1172/JCI113569
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10.1111/j.1432-1033.1985.tb09157.x
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10.1016/S0021-9258(18)61070-1
10.1111/j.1432-1033.1988.tb14308.x
{'key': 'e_1_2_1_11_2', 'first-page': '987', 'volume': '11', 'year': '1990', 'journal-title': 'Electrophoresis'} / Electrophoresis (1990)
10.1002/elps.1150121102
10.1002/elps.11501301197
10.1002/elps.1150121110
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10.1002/elps.11501301159
10.1093/clinchem/33.11.2011 / Clin. Chem. by Gianazza E. (1987)
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10.1002/elps.1150110412
10.1007/BF02193873
10.1016/0003-2697(77)90043-4
10.1007/BF00281458
10.1515/9783111439846-004 / Modern Methods in Protein Chemistry by Klose J. (1983)
10.1016/0165-022X(90)90044-D
10.1002/elps.1150091208
10.1093/carcin/13.7.1177
10.1038/227680a0
10.1002/elps.1150110709
10.1007/BF01024960
{'key': 'e_1_2_1_32_2', 'first-page': '155', 'volume-title': 'Modern Methods in Protein Chemistry', 'author': 'Ashman K.', 'year': '1985'} / Modern Methods in Protein Chemistry by Ashman K. (1985)
10.1007/BF02290390
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10.1002/elps.1150111017
10.1093/clinchem/28.4.739 / Clin. Chem. by Anderson N. G. (1982)
10.1007/BF01673702
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10.1016/S0021-9673(01)93213-3
10.1002/elps.1150140199

Dates

Type	When
Created	20 years, 3 months ago (May 28, 2005, 10:12 a.m.)
Deposited	1 year, 10 months ago (Oct. 24, 2023, 2:58 a.m.)
Indexed	1 year, 3 months ago (May 13, 2024, 11:15 p.m.)
Issued	31 years, 8 months ago (Jan. 1, 1994)
Published	31 years, 8 months ago (Jan. 1, 1994)
Published Online	20 years, 4 months ago (April 13, 2005)
Published Print	31 years, 8 months ago (Jan. 1, 1994)

Funders 0

None

BibTeX

@article{Jungblut_1994, title={Protein composition of the human heart: The construction of a myocardial two‐dimensional electrophoresis database}, volume={15}, ISSN={1522-2683}, url={http://dx.doi.org/10.1002/elps.1150150197}, DOI={10.1002/elps.1150150197}, number={1}, journal={ELECTROPHORESIS}, publisher={Wiley}, author={Jungblut, Peter and Otto, Albrecht and Zeindl‐Eberhart, Evelyn and Pleißner, Klaus‐P. and Knecht, Mathias and Regitz‐Zagrosek, Vera and Fleck, Eckart and Wittmann‐Liebold, Brigitte}, year={1994}, month=jan, pages={685–707} }

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