DOI: 10.1002/elps.1150010312. A comparison of commonly used discontinuous and continuous buffer systems for electrophoresis in sodium dodecyl sulfate‐containing polyacrylamide gels

A comparison of commonly used discontinuous and continuous buffer systems for electrophoresis in sodium dodecyl sulfate‐containing polyacrylamide gels

10.1002/elps.1150010312

Crossref journal-article

Wiley

ELECTROPHORESIS (311)

Abstract

AbstractA comparison between commonly used multiphasic zone electrophoresis (MZE) and continuous zone electrophoresis (CZE) buffer systems for polyacrylamide gel electrophoresis in sodium dodecyl sulfate (SDS‐PAGE) was performed. Some proteins behave differently in one or more of the buffer systems, indicating that buffer choice for SDS‐PAGE may be more important than previously recognized. Guidelines are suggested for choosing buffer systems for purposes other than molecular weight analysis.

Bibliography

Lanzillo, J. J., Stevens, J., & Fanburg, B. L. (1980). A comparison of commonly used discontinuous and continuous buffer systems for electrophoresis in sodium dodecyl sulfateâcontaining polyacrylamide gels. ELECTROPHORESIS, 1(3â4), 180â186. Portico.

Authors 3

Joseph J. Lanzillo (first)
Joanne Stevens (additional)
Barry L. Fanburg (additional)

References 24 Referenced 5

10.1016/0003-2697(80)90175-X
10.1016/0005-2795(73)90263-8
10.1042/bj1370575
10.1073/pnas.66.3.1002
10.1016/0304-4157(75)90016-7
10.1016/S0021-9258(19)44961-2
10.1016/0003-2697(79)90652-3
10.1016/S0021-9258(18)94310-3
10.1016/0076-6879(72)28007-7
10.1016/0003-2697(74)90201-2
10.1016/0005-2795(79)90114-4
{'key': 'e_1_2_1_13_2', 'first-page': '195', 'volume-title': "Electrophoresis '78", 'author': 'Lasky M.', 'year': '1978'} / Electrophoresis '78 by Lasky M. (1978)
10.1021/bi00729a014
10.1016/S0021-9258(18)94333-4
Fairbanks G. Steck T. L.andWallach D. F. H. Biochem.1971 2606–2616. (10.1021/bi00789a030)
10.1038/227680a0
10.1016/0076-6879(74)32012-5
{'key': 'e_1_2_1_19_2', 'first-page': '220', 'volume': '10', 'author': 'Maurer H. R.', 'year': '1972', 'journal-title': 'Z. Klin. Chem. Klin. Biochem.'} / Z. Klin. Chem. Klin. Biochem. by Maurer H. R. (1972)
10.1016/0005-2795(71)90094-8
10.1016/0003-2697(78)90389-5
10.1016/0003-2697(77)90107-5
10.1080/01496396908052249
10.1016/0003-9861(79)90232-7
10.1016/0003-2697(78)90281-6

Dates

Type	When
Created	20 years, 3 months ago (May 28, 2005, 8:05 a.m.)
Deposited	2 years ago (Aug. 31, 2023, 9:16 a.m.)
Indexed	1 day, 10 hours ago (Sept. 4, 2025, 10:11 a.m.)
Issued	45 years, 5 months ago (April 1, 1980)
Published	45 years, 5 months ago (April 1, 1980)
Published Online	20 years, 4 months ago (April 13, 2005)
Published Print	45 years, 5 months ago (April 1, 1980)

Funders 0

None

BibTeX

@article{Lanzillo_1980, title={A comparison of commonly used discontinuous and continuous buffer systems for electrophoresis in sodium dodecyl sulfate‐containing polyacrylamide gels}, volume={1}, ISSN={1522-2683}, url={http://dx.doi.org/10.1002/elps.1150010312}, DOI={10.1002/elps.1150010312}, number={3–4}, journal={ELECTROPHORESIS}, publisher={Wiley}, author={Lanzillo, Joseph J. and Stevens, Joanne and Fanburg, Barry L.}, year={1980}, month=apr, pages={180–186} }

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