DOI: 10.1002/elan.201000614. Direct Electrochemistry and Electrocatalysis of Horseradish Peroxidase Immobilized on Water Soluble Sulfonated Graphene Film via Self‐assembly

Direct Electrochemistry and Electrocatalysis of Horseradish Peroxidase Immobilized on Water Soluble Sulfonated Graphene Film via Self‐assembly

10.1002/elan.201000614

Crossref journal-article

Wiley

Electroanalysis (311)

Abstract

AbstractA novel water soluble sulfonated graphene (denoted as sulfonated‐G), was synthesized for the immobilization and biosensing of horseradish peroxidase (HRP). Compared with unmodified graphene, sulfonated‐G could not only well disperse in aqueous solution as individual nanosheet, but also exhibit apparent negative charge. Using a simple self‐assembly method, HRP was immobilized on sulfonated‐G at the surface of GC electrode to form HRP/sulfonated‐G/GC electrode under mild condition. The immobilized HRP showed enhanced direct electron transfer for the HRP‐Fe(III)/Fe(II) redox centre. Based on the direct electron transfer of the immobilized HRP, HRP/sulfonated‐G/GC electrode exhibited excellent catalytic performance for H2O2 and NaNO2 reduction. Accordingly, the proposed method not only simplifies the methodology of enzyme immobilization, but also provides an efficient platform for fabricating novel biosensors and bioelectronic devices.

Bibliography

Zhang, Q., Qiao, Y., Zhang, L., Wu, S., Zhou, H., Xu, J., & Song, X. (2011). Direct Electrochemistry and Electrocatalysis of Horseradish Peroxidase Immobilized on Water Soluble Sulfonated Graphene Film via Selfâassembly. Electroanalysis, 23(4), 900â906. Portico.

Authors 7

Qian Zhang (first)
Yun Qiao (additional)
Ling Zhang (additional)
Shuyao Wu (additional)
Hua Zhou (additional)
Jingwei Xu (additional)
Xi‐Ming Song (additional)

References 39 Referenced 33

Dates

Type	When
Created	14 years, 6 months ago (March 1, 2011, 8:02 p.m.)
Deposited	1 year, 11 months ago (Sept. 12, 2023, 9:04 a.m.)
Indexed	1 year, 2 months ago (June 10, 2024, 9:30 p.m.)
Issued	14 years, 6 months ago (March 1, 2011)
Published	14 years, 6 months ago (March 1, 2011)
Published Online	14 years, 6 months ago (March 1, 2011)
Published Print	14 years, 5 months ago (April 1, 2011)

Funders 0

None

BibTeX

@article{Zhang_2011, title={Direct Electrochemistry and Electrocatalysis of Horseradish Peroxidase Immobilized on Water Soluble Sulfonated Graphene Film via Self‐assembly}, volume={23}, ISSN={1521-4109}, url={http://dx.doi.org/10.1002/elan.201000614}, DOI={10.1002/elan.201000614}, number={4}, journal={Electroanalysis}, publisher={Wiley}, author={Zhang, Qian and Qiao, Yun and Zhang, Ling and Wu, Shuyao and Zhou, Hua and Xu, Jingwei and Song, Xi‐Ming}, year={2011}, month=mar, pages={900–906} }

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  "abstract": "<jats:title>Abstract</jats:title><jats:p>A novel water soluble sulfonated graphene (denoted as sulfonated\u2010G), was synthesized for the immobilization and biosensing of horseradish peroxidase (HRP). Compared with unmodified graphene, sulfonated\u2010G could not only well disperse in aqueous solution as individual nanosheet, but also exhibit apparent negative charge. Using a simple self\u2010assembly method, HRP was immobilized on sulfonated\u2010G at the surface of GC electrode to form HRP/sulfonated\u2010G/GC electrode under mild condition. The immobilized HRP showed enhanced direct electron transfer for the HRP\u2010Fe(III)/Fe(II) redox centre. Based on the direct electron transfer of the immobilized HRP, HRP/sulfonated\u2010G/GC electrode exhibited excellent catalytic performance for H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> and NaNO<jats:sub>2</jats:sub> reduction. Accordingly, the proposed method not only simplifies the methodology of enzyme immobilization, but also provides an efficient platform for fabricating novel biosensors and bioelectronic devices.</jats:p>",
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