DOI: 10.1002/elan.1140030306. An electrochemical immunoassay for <i>Clostridium perfringens</i> phosholipase C

An electrochemical immunoassay for Clostridium perfringens phosholipase C

10.1002/elan.1140030306

Crossref journal-article

Wiley

Electroanalysis (311)

Abstract

AbstractA sensitive two‐site, enzyme‐linked immunoassay utilizing electrochemical detection has been developed for Clostridium perfringens phospholipase C (α toxin). Alkaline phosphatase conjugated to a mouse monoclonal antibody is used as the enzyme label. The enzyme activity is measured using 1‐naphthyl phosphate as the enzyme substrate. The enzyme‐generated naphthol is detected amperometrically in a thin‐layer cell at a glassy carbon electrode after chromatography on a 50 mm C8 reverse phase column. Two assay formats are described, based on either microtiter plates or polydispersed polymeric microbeads. The limits of detection of the two assay formats were 67.1 and 13.0 ng/mL, respectively, with a total assay time of less than one hour. The performance characteristics of three possible substrates for the alkaline phosphatase catalyzed reaction are also described.

Bibliography

Cardosi, M., Birch, S., Talbot, J., & Phillips, A. (1991). An electrochemical immunoassay for Clostridium perfringens phosholipase C. Electroanalysis, 3(3), 169â176. Portico.

Authors 4

Marco Cardosi (first)
Stephen Birch (additional)
Jane Talbot (additional)
Anthony Phillips (additional)

References 21 Referenced 18

{'key': 'e_1_2_1_2_2', 'first-page': '48', 'volume-title': "Topley and Wilson's Principles of Bacteriology, Virology and Immunity", 'author': 'Smith J. W. G.', 'year': '1984'} / Topley and Wilson's Principles of Bacteriology, Virology and Immunity by Smith J. W. G. (1984)
10.1093/clinids/1.2.254
10.1042/bj0350884
10.1111/j.1432-1033.1972.tb01832.x
10.1016/0014-5793(75)80800-3
10.1016/0076-6879(81)71084-X
10.1016/0076-6879(81)71085-1
10.1016/0005-2760(78)90199-6
{'key': 'e_1_2_1_10_2', 'first-page': '367', 'volume-title': 'Bacterial Toxins and Cell membranes', 'author': 'Molby R.', 'year': '1978'} / Bacterial Toxins and Cell membranes by Molby R. (1978)
10.3109/15569548209019476
10.1093/infdis/157.2.272
10.1007/BF01872421
10.1016/0005-2736(75)90216-3
A. D.Shuttleworth D.Peroival A. J.Logan R. W.Titball andD. C.Kelly Infect. Immun.in Press.
10.1016/0022-1759(83)90104-7
10.1021/ac00292a033
10.1007/978-94-009-2895-4_23
10.1021/ac60214a047
{'key': 'e_1_2_1_20_2', 'first-page': '31', 'volume': '54', 'author': 'Millan J. L.', 'year': '1985', 'journal-title': 'Clin. Chem.'} / Clin. Chem. by Millan J. L. (1985)
10.1016/S0021-9258(18)68247-X
10.1111/j.1574-6968.1989.tb03104.x

Dates

Type	When
Created	20 years, 3 months ago (May 29, 2005, 6:42 a.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 5:16 p.m.)
Indexed	1 year, 10 months ago (Oct. 23, 2023, 12:58 p.m.)
Issued	34 years, 5 months ago (April 1, 1991)
Published	34 years, 5 months ago (April 1, 1991)
Published Online	20 years, 4 months ago (April 9, 2005)
Published Print	34 years, 5 months ago (April 1, 1991)

Funders 0

None

BibTeX

@article{Cardosi_1991, title={An electrochemical immunoassay for Clostridium perfringens phosholipase C}, volume={3}, ISSN={1521-4109}, url={http://dx.doi.org/10.1002/elan.1140030306}, DOI={10.1002/elan.1140030306}, number={3}, journal={Electroanalysis}, publisher={Wiley}, author={Cardosi, Marco and Birch, Stephen and Talbot, Jane and Phillips, Anthony}, year={1991}, month=apr, pages={169–176} }

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