DOI: 10.1002/eji.1830261102. Differential nuclear localization of p50, p52, and RelB proteins in human accessory cells of the immune response <i>in situ</i>

Differential nuclear localization of p50, p52, and RelB proteins in human accessory cells of the immune response in situ

10.1002/eji.1830261102

Crossref journal-article

Wiley

European Journal of Immunology (311)

Abstract

AbstractThe Rel/NF‐ϰB proteins, p50, p52, p65, c‐Rel, and RelB, constitute a family of transcription factors involved in the positive regulation of a variety of genes during the immune response. Recently, it has been shown that RelB knockout mice have no dendritic cells (DC). An overexpression of p50 has been described in follicular dendritic cells (FDC). A constitutive NF‐ϰB activity has been reported in mature macrophages. This led to the hypothesis that some of the Rel/NF‐ϰB proteins were key nuclear factors in functions of accessory cells of the immune response. Therefore, we investigated in situ the nuclear localization of Rel/NF‐ϰB proteins in accessory cells of the immune system by immunohistochemistry and double labeling by immunofluorescence from five normal human tonsils and five lymph nodes with follicular hyperplasia. Nuclear p65 and c‐Rel proteins were found in all cell types including lymphocytes. In germinal centers GC, p50, p52, and RelB were found in the nuclei of FDC only and were not detected in the nuclei of CD68+ cells. In T cell areas, p50, p52, and RelB were found in the nuclei of HLA‐DR+ cells with an antigen‐presenting cell (APC) morphology. p52 and RelB were detected in the nuclei in both CDla+ and CD68+ cells from the T cell area, whereas p50 was found only in CD68− and CDla− cells. Cells with nuclear p50 were negative for the CD38, CD20 and CD2 markers. These results show that, physiologically, high levels of nuclear of p50, p52 and RelB are restricted to accessory cells of the immune system, which include FDC in GC, and DC and macrophages in the T cell zone, that specialized scavenger macrophages from GC do not have detectable levels of p52 and RelB, whereas macrophages from the T cell area, known to present the antigen to T cells, do have both nuclear p52 and RelB, and that in the T cell zone, p52 and RelB are located in nuclei of both CDla+, CD68+ or both, cells APC, whereas p50 is restricted to CDla− and CD68− APC. The different patterns of p50, p52 and RelB protein nuclear localization may provide insight into their different roles during the immune response in vivo.

Bibliography

Feuillard, J., KÃ¶rner, M., Israel, A., Vassy, J., & Raphael, M. (1996). Differential nuclear localization of p50, p52, and RelB proteins in human accessory cells of the immune response in situ. European Journal of Immunology, 26(11), 2547â2551. Portico.

Authors 5

Jean Feuillard (first)
Marie Körner (additional)
Alain Israel (additional)
Jeanny Vassy (additional)
Martine Raphael (additional)

References 21 Referenced 36

10.1016/0092-8674(95)90416-6
10.1038/373531a0
10.4049/jimmunol.152.1.12 / J. Immunol. by Feuillard J. (1994)
10.1128/MCB.10.4.1498
10.1016/0022-1759(84)90334-X
10.4049/jimmunol.134.3.1360 / J. Immunol. by Schnizlein C. T. (1984)
10.1146/annurev.iy.09.040191.001415
10.1016/0092-8674(90)90275-J
{'key': 'e_1_2_1_10_2', 'first-page': '322', 'volume': '54', 'author': 'Franklin W. A.', 'year': '1986', 'journal-title': 'Lab. Invest.'} / Lab. Invest. by Franklin W. A. (1986)
{'key': 'e_1_2_1_11_2', 'first-page': '73', 'volume': '119', 'author': 'Wood G. S.', 'year': '1985', 'journal-title': 'Am. J. Pathol.'} / Am. J. Pathol. by Wood G. S. (1985)
10.1128/jvi.69.8.4737-4745.1995 / J. Virol. by McIlroy D. (1995)
10.1084/jem.178.3.1067
10.1016/0167-5699(95)80061-1
{'key': 'e_1_2_1_15_2', 'first-page': '1003', 'volume': '10', 'author': 'Dobrzanski P.', 'year': '1995', 'journal-title': 'Oncogene'} / Oncogene by Dobrzanski P. (1995)
{'key': 'e_1_2_1_16_2', 'first-page': '3289', 'volume': '9', 'author': 'Weih F.', 'year': '1994', 'journal-title': 'Oncogene'} / Oncogene by Weih F. (1994)
10.1016/0167-5699(96)80544-5
10.1111/j.1600-065X.1992.tb00635.x
10.1128/MCB.9.2.844
10.1128/MCB.14.5.2926
{'key': 'e_1_2_1_21_2', 'first-page': '135', 'volume': '47', 'author': 'Chen C. C.', 'year': '1995', 'journal-title': 'Agents & Actions (Suppl.)'} / Agents & Actions (Suppl.) by Chen C. C. (1995)
10.1096/fasebj.9.10.7542214

Dates

Type	When
Created	18 years, 5 months ago (March 1, 2007, 8:32 p.m.)
Deposited	1 year, 10 months ago (Oct. 27, 2023, 10:47 a.m.)
Indexed	4 months, 4 weeks ago (March 31, 2025, 9:46 a.m.)
Issued	28 years, 9 months ago (Nov. 1, 1996)
Published	28 years, 9 months ago (Nov. 1, 1996)
Published Online	19 years, 9 months ago (Nov. 17, 2005)
Published Print	28 years, 9 months ago (Nov. 1, 1996)

Funders 0

None

BibTeX

@article{Feuillard_1996, title={Differential nuclear localization of p50, p52, and RelB proteins in human accessory cells of the immune response in situ}, volume={26}, ISSN={1521-4141}, url={http://dx.doi.org/10.1002/eji.1830261102}, DOI={10.1002/eji.1830261102}, number={11}, journal={European Journal of Immunology}, publisher={Wiley}, author={Feuillard, Jean and Körner, Marie and Israel, Alain and Vassy, Jeanny and Raphael, Martine}, year={1996}, month=nov, pages={2547–2551} }

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