DOI: 10.1002/eji.1830200211. The implications of subunit interactions for the structure of the T cell receptor‐CD3 complex

The implications of subunit interactions for the structure of the T cell receptor‐CD3 complex

10.1002/eji.1830200211

Crossref journal-article

Wiley

European Journal of Immunology (311)

Abstract

AbstractCell surface‐expressed receptors are often multichain complexes. One of these, the T cell receptor (TcR)αβ‐CD3 complex, is known to contain at least seven chains: the α and β TcR chains plus the γ, δ, ϵ and two ζ chains from the CD3 complex (αβγδϵζ2). To gain insight into the structure of the complex we have used anti‐peptide antisera specific for the individual subunits of the complex, and nonionic and ionic detergents to determine subunit interactions within the complex. Four closely associated pairs of chains could be identified: αβ, ζ2, γϵ and δϵ. Interactions between the TcR αβ and either γϵ or δϵ could be observed in the apparent absence of other CD3 chains. Furthermore, a hierarchy in the strength of the association between the TcR and the individual CD3 chains could be distinguished: TcR ϵ > TcR δ > TcR γ. The ζ2 dimer could only be detected in “intact” TcR‐CD3 complexes shedding no light on possible interactions with either the TcR or CD3‐γ, δ and ϵ chains. Finally, cross‐linking experiments suggest a close spatial relationship between the TcR αβ and both the CD3‐γ and CD3‐ϵ chains. The results demonstrate that the methods used give valuable information on subunit interactions in a cell surface‐expressed receptor complex and suggest a TcR‐CD3 complex in which two ϵ chains are present, one linked to γ and the other to δ. The data further indicate that γϵ and δϵ complexes interact directly with the TcR chains. Based on the observations a model for the structure of the TcR‐CD3 is presented and discussed.

Bibliography

Koning, F., Lee Maloy, W., & Coligan, J. E. (1990). The implications of subunit interactions for the structure of the T cell receptorâCD3 complex. European Journal of Immunology, 20(2), 299â305. Portico.

Authors 3

Frits Koning (first)
W. Lee Maloy (additional)
John E. Coligan (additional)

References 32 Referenced 88

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Dates

Type	When
Created	18 years, 5 months ago (March 1, 2007, 6:48 a.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 12:37 p.m.)
Indexed	4 months, 3 weeks ago (March 30, 2025, 7:03 p.m.)
Issued	35 years, 6 months ago (Feb. 1, 1990)
Published	35 years, 6 months ago (Feb. 1, 1990)
Published Online	19 years, 9 months ago (Nov. 17, 2005)
Published Print	35 years, 6 months ago (Feb. 1, 1990)

Funders 0

None

BibTeX

@article{Koning_1990, title={The implications of subunit interactions for the structure of the T cell receptor‐CD3 complex}, volume={20}, ISSN={1521-4141}, url={http://dx.doi.org/10.1002/eji.1830200211}, DOI={10.1002/eji.1830200211}, number={2}, journal={European Journal of Immunology}, publisher={Wiley}, author={Koning, Frits and Lee Maloy, W. and Coligan, John E.}, year={1990}, month=feb, pages={299–305} }

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