DOI: 10.1002/eji.1830191202. The lymphocyte‐specific protein tyrosine kinase p56<sup><i>lck</i></sup> is hyperphosphorylated on serine and tyrosine residues within minutes after activation via T cell receptor or CD2

The lymphocyte‐specific protein tyrosine kinase p56lck is hyperphosphorylated on serine and tyrosine residues within minutes after activation via T cell receptor or CD2

10.1002/eji.1830191202

Crossref journal-article

Wiley

European Journal of Immunology (311)

Abstract

AbstractHuman T cells can be activated and induced to proliferate through either the antigen‐specific receptor complex (TcR‐CD3) or the CD2 surface molecule. Following stimulation, both serine and tyrosine phosphorylation of cellular protein have been demonstrated to occur. p56lck, a protein tyrosine kinase associated to the inner face of the plasma membrane, is almost exclusively expressed in lymphoid cells, especially T cells. Within minutes after activation of a human T cell‐derived line (Jurkat) via stimulation of either the TcR‐CD3 complex or the CD2 glycoprotein, we observed a hyperphorphosylation of p56lck. A concomitant shift to a higher molecular weight in sodium dodecyl sulfatepolyacrylamide gel was also observed. Similar changes were obtained with phorbol 12‐myristate 13‐acetate. Tryptic phosphopeptide analysis of the hyperphosphorylated form of p56lck yielded new phosphorylated sites in serine residues and an increased tyrosine phosphorylation. These results suggest that p56lck may be intimately connected to the signaling pathway in T cell activation.

Bibliography

Danielian, S., Fagard, R., Alcover, A., Acuto, O., & Fischer, S. (1989). The lymphocyteâspecific protein tyrosine kinase p56lck is hyperphosphorylated on serine and tyrosine residues within minutes after activation via T cell receptor or CD2. European Journal of Immunology, 19(12), 2183â2189. Portico.

Authors 5

Silvia Danielian (first)
Remi Fagard (additional)
AndrÉS Alcover (additional)
Oreste Acuto (additional)
Siegmund Fischer (additional)

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Dates

Type	When
Created	18 years, 5 months ago (March 1, 2007, 3:04 a.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 1:19 a.m.)
Indexed	1 month, 3 weeks ago (June 27, 2025, 9:45 a.m.)
Issued	35 years, 8 months ago (Dec. 1, 1989)
Published	35 years, 8 months ago (Dec. 1, 1989)
Published Online	19 years, 8 months ago (Dec. 1, 2005)
Published Print	35 years, 8 months ago (Dec. 1, 1989)

Funders 0

None

BibTeX

@article{Danielian_1989, title={The lymphocyte‐specific protein tyrosine kinase p56lck is hyperphosphorylated on serine and tyrosine residues within minutes after activation via T cell receptor or CD2}, volume={19}, ISSN={1521-4141}, url={http://dx.doi.org/10.1002/eji.1830191202}, DOI={10.1002/eji.1830191202}, number={12}, journal={European Journal of Immunology}, publisher={Wiley}, author={Danielian, Silvia and Fagard, Remi and Alcover, AndrÉS and Acuto, Oreste and Fischer, Siegmund}, year={1989}, month=dec, pages={2183–2189} }

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