DOI: 10.1002/eji.1830090410. Mac‐1: a macrophage differentiation antigen identified by monoclonal antibody

Mac‐1: a macrophage differentiation antigen identified by monoclonal antibody

10.1002/eji.1830090410

Crossref journal-article

Wiley

European Journal of Immunology (311)

Abstract

AbstractWe have previously described the derivation of M1/70, a hybrid myeloma line secreting monoclonal rat anti‐mouse cell surface antibody (Springer, T., Galfre, G., Secher, D. S. and Milstein, C, Eur. J. Immunol. 1978. 8: 539). We have now investigated the cellular distribution of this antigen using a 125I‐labeled anti‐rat IgG indirect binding assay, the fluorescence‐activated cell sorter, autoradiography and precipitation of cell surface molecules. Screening with a tumor cell panel showed strong reactivity with a macrophage‐like line but no reactivity with B or T lymphoma lines. In normal tissues, M1/70 antigen was found to be present in small amounts on spleen and exudate granulocytes and a subpopulation of bone marrow cells, in moderate amounts on spleen and blood monocytes and expressed in much larger amounts on spleen histiocytes and peritoneal exudate macrophages. In contrast, M1/70 antigen was found to be absent from erythroid and lymphoid cells. M1/70 antibody precipitated two polypeptides of 190 000 and 105 000 mol. wt. which were present in much greater amounts on peritoneal exudate macrophages than on spleen cells. The expression on phagocytes of two other antigens identified by monoclonal antibodies M1/69 and M1/9.3 was also examined. Monocytes and granulocytes expressed large amounts of M1/69 and low amounts of M1/70 antigen, while in peritoneal exudate macrophages this pattern was dramatically reversed. M1/70 thus defines a differentiation antigen on mononuclear phagocytes and granulocytes, the expression of which is specifically increased during monocyte maturation. This antibody is the first to be described which recognizes a discrete molecule specific to phagocytes.

Bibliography

Springer, T., GalfrÃ©, G., Secher, D. S., & Milstein, C. (1979). Macâ1: a macrophage differentiation antigen identified by monoclonal antibody. European Journal of Immunology, 9(4), 301â306. Portico.

Authors 4

Timothy Springer (first)
Giovanni Galfré (additional)
David S. Secher (additional)
Cesar Milstein (additional)

References 29 Referenced 762

10.1002/eji.1830080802
10.1016/0092-8674(78)90333-1
Milstein C. Galfré G. Secher D. S.andSpringer T. In Genetics and Human Biology: Possibilities and Realities Ciba Foundation Symposium 66 London1978 in press.
{'key': 'e_1_2_1_5_2', 'first-page': '45', 'volume': '81', 'author': 'Springer T.', 'year': '1978', 'journal-title': 'Curr. Top. Microbiol. Immunol.'} / Curr. Top. Microbiol. Immunol. by Springer T. (1978)
10.1016/0092-8674(75)90179-8
10.1007/978-3-642-81083-1_12
10.1093/jnci/54.5.1249
{'key': 'e_1_2_1_9_2', 'first-page': '603', 'volume': '33', 'author': 'Dawe C. J.', 'year': '1957', 'journal-title': 'Am. J. Pathol.'} / Am. J. Pathol. by Dawe C. J. (1957)
{'key': 'e_1_2_1_10_2', 'first-page': '23', 'volume-title': 'Handbook of Experimental Immunology', 'author': 'Ford W. L.', 'year': '1978'} / Handbook of Experimental Immunology by Ford W. L. (1978)
10.1016/0022-1759(75)90026-5
10.1002/eji.1830031007
{'key': 'e_1_2_1_13_2', 'first-page': '15', 'volume-title': 'Handbook of Experimental Immunology', 'author': 'Johnson G. D.', 'year': '1978'} / Handbook of Experimental Immunology by Johnson G. D. (1978)
10.1038/266550a0
{'key': 'e_1_2_1_15_2', 'first-page': '22', 'volume-title': 'Handbook of Experimental Immunology', 'author': 'Herzenberg L. A.', 'year': '1978'} / Handbook of Experimental Immunology by Herzenberg L. A. (1978)
10.4049/jimmunol.115.1.261 / J. Immunol. by Martz E. (1975)
10.1073/pnas.72.1.157
10.1016/0090-1229(78)90204-0
10.1101/SQB.1977.041.01.010
{'key': 'e_1_2_1_20_2', 'first-page': '161', 'volume-title': 'Mononuclear Phagocytes in Immunity, Infection and Pathology', 'author': 'Van Furth R.', 'year': '1975'} / Mononuclear Phagocytes in Immunity, Infection and Pathology by Van Furth R. (1975)
{'key': 'e_1_2_1_21_2', 'first-page': '471', 'volume-title': 'The White Cell', 'author': 'Cline M. J.', 'year': '1975'} / The White Cell by Cline M. J. (1975)
10.1084/jem.145.1.187
10.4049/jimmunol.114.2_Part_2.894
10.1038/218036a0
10.4049/jimmunol.102.3.656 / J. Immunol. by Hirsch M. S. (1969)
{'key': 'e_1_2_1_26_2', 'first-page': '431', 'volume': '22', 'author': 'Gallily R.', 'year': '1972', 'journal-title': 'Immunology'} / Immunology by Gallily R. (1972)
10.1038/icb.1973.75
10.4049/jimmunol.116.2.273 / J. Immunol. by Stinnett J. D. (1976)
10.1002/eji.1830070710
10.1073/pnas.75.6.2844

Dates

Type	When
Created	18 years, 6 months ago (Feb. 28, 2007, 5:39 a.m.)
Deposited	1 year, 10 months ago (Nov. 2, 2023, 12:29 p.m.)
Indexed	18 hours, 12 minutes ago (Sept. 3, 2025, 7:13 a.m.)
Issued	46 years, 5 months ago (April 1, 1979)
Published	46 years, 5 months ago (April 1, 1979)
Published Online	19 years, 9 months ago (Nov. 16, 2005)
Published Print	46 years, 5 months ago (April 1, 1979)

Funders 0

None

BibTeX

@article{Springer_1979, title={Mac‐1: a macrophage differentiation antigen identified by monoclonal antibody}, volume={9}, ISSN={1521-4141}, url={http://dx.doi.org/10.1002/eji.1830090410}, DOI={10.1002/eji.1830090410}, number={4}, journal={European Journal of Immunology}, publisher={Wiley}, author={Springer, Timothy and Galfré, Giovanni and Secher, David S. and Milstein, Cesar}, year={1979}, month=apr, pages={301–306} }

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