DOI: 10.1002/cyto.a.20040. A model‐based approach for automated in vitro cell tracking and chemotaxis analyses

A model‐based approach for automated in vitro cell tracking and chemotaxis analyses

10.1002/cyto.a.20040

Crossref journal-article

Wiley

Cytometry Part A (311)

Abstract

AbstractBackgroundChemotaxis may be studied in two main ways: 1) counting cells passing through an insert (e.g., using Boyden chambers), and 2) directly observing cell cultures (e.g., using Dunn chambers), both in response to stationary concentration gradients. This article promotes the use of Dunn chambers and in vitro cell‐tracking, achieved by video microscopy coupled with automatic image analysis software, in order to extract quantitative and qualitative measurements characterizing the response of cells to a diffusible chemical agent.MethodsPreviously, we set up a videomicroscopy system coupled with image analysis software that was able to compute cell trajectories from in vitro cell cultures. In the present study, we are introducing a new software increasing the application field of this system to chemotaxis studies. This software is based on an adapted version of the active contour methodology, enabling each cell to be efficiently tracked for hours and resulting in detailed descriptions of individual cell trajectories. The major advantages of this method come from an improved robustness with respect to variability in cell morphologies between different cell lines and dynamical changes in cell shape during cell migration. Moreover, the software includes a very small number of parameters which do not require overly sensitive tuning. Finally, the running time of the software is very short, allowing improved possibilities in acquisition frequency and, consequently, improved descriptions of complex cell trajectories, i.e. trajectories including cell division and cell crossing.ResultsWe validated this software on several artificial and real cell culture experiments in Dunn chambers also including comparisons with manual (human‐controlled) analyses.ConclusionsWe developed new software and data analysis tools for automated cell tracking which enable cell chemotaxis to be efficiently analyzed. © 2004 Wiley‐Liss, Inc.

Bibliography

Debeir, O., Camby, I., Kiss, R., Van Ham, P., & Decaestecker, C. (2004). A modelâbased approach for automated in vitro cell tracking and chemotaxis analyses. Cytometry Part A, 60A(1), 29â40. Portico.

Authors 5

Olivier Debeir (first)
Isabelle Camby (additional)
Robert Kiss (additional)
Philippe Van Ham (additional)
Christine Decaestecker (additional)

References 21 Referenced 38

10.1006/bbrc.1997.6291
10.1002/(SICI)1097-4695(19981115)37:3<373::AID-NEU3>3.0.CO;2-H
10.1002/(SICI)1097-0320(19990501)36:1<1::AID-CYTO1>3.0.CO;2-P
10.1007/BF00133570
10.1006/gmip.1997.0453
10.1083/jcb.60.3.673
{'key': 'e_1_2_5_8_2', 'first-page': '545', 'article-title': 'Setting up of an original computer‐assisted methodology to characterize in vitro drug‐induced anti‐angiogenic effects', 'volume': '2', 'author': 'Farinelle S', 'year': '1998', 'journal-title': 'Int J Mol Med'} / Int J Mol Med / Setting up of an original computer‐assisted methodology to characterize in vitro drug‐induced anti‐angiogenic effects by Farinelle S (1998)
10.1016/S1056-8719(00)00080-0
10.1242/jcs.99.4.769
10.1242/jcs.109.4.793
10.1038/labinvest.3780407
10.1038/labinvest.3780269
10.1136/gut.50.3.392
10.1002/9780470316979
10.1093/biomet/67.1.175
{'key': 'e_1_2_5_17_2', 'first-page': '323', 'volume-title': 'Biology of the chemotactic response', 'author': 'Wilkinson PC', 'year': '1990'} / Biology of the chemotactic response by Wilkinson PC (1990)
10.1083/jcb.141.5.1147
10.1046/j.1365-2141.1997.d01-2080.x
10.1046/j.1365-2141.1998.00767.x
{'key': 'e_1_2_5_21_2', 'first-page': '5301', 'article-title': 'Fully automated three‐dimensional tracking of cancer cells in collagen gels: determination of motility phenotypes at the cellular level', 'volume': '62', 'author': 'Demou ZN', 'year': '2002', 'journal-title': 'Cancer Res'} / Cancer Res / Fully automated three‐dimensional tracking of cancer cells in collagen gels: determination of motility phenotypes at the cellular level by Demou ZN (2002)
10.1002/cm.10048

Dates

Type	When
Created	21 years, 2 months ago (June 9, 2004, 2:04 p.m.)
Deposited	1 year, 10 months ago (Oct. 13, 2023, 3:30 p.m.)
Indexed	1 year, 9 months ago (Nov. 21, 2023, 10:53 p.m.)
Issued	21 years, 2 months ago (June 9, 2004)
Published	21 years, 2 months ago (June 9, 2004)
Published Online	21 years, 2 months ago (June 9, 2004)
Published Print	21 years, 1 month ago (July 1, 2004)

Funders 0

None

BibTeX

@article{Debeir_2004, title={A model‐based approach for automated in vitro cell tracking and chemotaxis analyses}, volume={60A}, ISSN={1552-4930}, url={http://dx.doi.org/10.1002/cyto.a.20040}, DOI={10.1002/cyto.a.20040}, number={1}, journal={Cytometry Part A}, publisher={Wiley}, author={Debeir, Olivier and Camby, Isabelle and Kiss, Robert and Van Ham, Philippe and Decaestecker, Christine}, year={2004}, month=jun, pages={29–40} }

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