DOI: 10.1002/cyto.990110123. Study of X chromosome abnormality in XX males using bivariate flow karyotype analysis and flow sorted dot blots

Study of X chromosome abnormality in XX males using bivariate flow karyotype analysis and flow sorted dot blots

10.1002/cyto.990110123

Crossref journal-article

Wiley

Cytometry (311)

Abstract

AbstractWe have used bivariate flow karyotype analysis to quantify aberrant X chromosome size in 11 XX males. With one exception, the patients could be grouped into those with an X homologue difference greater than normal (Group A, n = 3) and into those whose X homologue difference could not be distinguished from female controls (Group B, n = 7). The range of sizes of the aberrant X chromosome in Y‐sequence positive patients agrees with the variable nature of the X‐Y interchange in these individuals as determined by the use of Y‐specific DNA probes and Southern blotting analysis. In one patient it was possible to sort separately the normal and the X‐Y interchanged homologues for dot blot analysis. The presence of Y sequences and an increased dose of the zinc finger gene, ZFY, were detected in the X‐Y interchanged homologue. In preliminary studies of 5 male and 6 female controls, it was noted that a consistent difference between the two X homologues in females was found which could not be totally explained by errors of the fitting procedure. We suggest that this difference could be due to X inactivation and that the two X homologues in females might be distinguishable.

Bibliography

Carter, N. P., FergusonâSmith, M. E., Affara, N. A., Briggs, H., & FergusonâSmith, M. A. (1990). Study of X chromosome abnormality in XX males using bivariate flow karyotype analysis and flow sorted dot blots. Cytometry, 11(1), 202â207. Portico.

Authors 5

N. P. Carter (first)
M. E. Ferguson‐Smith (additional)
N. A. Affara (additional)
H. Briggs (additional)
M. A. Ferguson‐Smith (additional)

References 32 Referenced 32

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{'key': 'e_1_2_1_7_2', 'first-page': '85', 'article-title': 'Localization of Y chromosome sequences in normal and XX males', 'volume': '61', 'author': 'Buckel V. J.', 'year': '1987', 'journal-title': 'J Med Genet'} / J Med Genet / Localization of Y chromosome sequences in normal and XX males by Buckel V. J. (1987)
10.1111/j.0954-6820.1964.tb04630.x / Acta Med Scand [Suppl] / XX chromosomes in a human male; First case by de la Chapelle A. (1964)
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10.1007/BF00277683 / Hum Genet / Heteromorphic X chromosomes in 46 XX males: Evidence for involvement of X‐Y interchange by Evans H. J. (1979)
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{'key': 'e_1_2_1_12_2', 'first-page': '153', 'article-title': 'Analysis of variable X‐Y interchange in XX males', 'volume': '37', 'author': 'Ferguson‐Smith M. A.', 'year': '1985', 'journal-title': 'Am J Hum Genet'} / Am J Hum Genet / Analysis of variable X‐Y interchange in XX males by Ferguson‐Smith M. A. (1985)
{'key': 'e_1_2_1_13_2', 'first-page': '41', 'article-title': 'Ordering of Y‐specific sequences by deletion mapping and analysis of X‐Y interchange males and females', 'volume': '101', 'author': 'Ferguson‐Smith M. A.', 'year': '1987', 'journal-title': 'Development [Suppl]'} / Development [Suppl] / Ordering of Y‐specific sequences by deletion mapping and analysis of X‐Y interchange males and females by Ferguson‐Smith M. A. (1987)
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{'key': 'e_1_2_1_21_2', 'first-page': '1025', 'article-title': 'Y short arm material translocated to distal X short arm in XX males: Evidence from in situ hybridisation of a Y specific single copy DNA probe', 'volume': '36', 'author': 'Magenis E.', 'year': '1984', 'journal-title': 'Am J Hum Genet'} / Am J Hum Genet / Y short arm material translocated to distal X short arm in XX males: Evidence from in situ hybridisation of a Y specific single copy DNA probe by Magenis E. (1984)
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Dates

Type	When
Created	20 years ago (Aug. 8, 2005, 10:24 a.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 4:22 p.m.)
Indexed	1 month, 3 weeks ago (July 12, 2025, 6:54 p.m.)
Issued	35 years, 8 months ago (Jan. 1, 1990)
Published	35 years, 8 months ago (Jan. 1, 1990)
Published Online	20 years, 2 months ago (June 21, 2005)
Published Print	35 years, 8 months ago (Jan. 1, 1990)

Funders 0

None

BibTeX

@article{Carter_1990, title={Study of X chromosome abnormality in XX males using bivariate flow karyotype analysis and flow sorted dot blots}, volume={11}, ISSN={1097-0320}, url={http://dx.doi.org/10.1002/cyto.990110123}, DOI={10.1002/cyto.990110123}, number={1}, journal={Cytometry}, publisher={Wiley}, author={Carter, N. P. and Ferguson‐Smith, M. E. and Affara, N. A. and Briggs, H. and Ferguson‐Smith, M. A.}, year={1990}, month=jan, pages={202–207} }

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