DOI: 10.1002/cphc.201100686. Three‐Dimensional Super‐Resolution Imaging of the Midplane Protein FtsZ in Live <i>Caulobacter crescentus</i> Cells Using Astigmatism

Three‐Dimensional Super‐Resolution Imaging of the Midplane Protein FtsZ in Live Caulobacter crescentus Cells Using Astigmatism

10.1002/cphc.201100686

Crossref journal-article

Wiley

ChemPhysChem (311)

Abstract

AbstractSingle‐molecule super‐resolution imaging provides a non‐invasive method for nanometer‐scale imaging and is ideally suited to investigations of quasi‐static structures within live cells. Here, we extend the ability to image subcellular features within bacteria cells to three dimensions based on the introduction of a cylindrical lens in the imaging pathway. We investigate the midplane protein FtsZ in Caulobacter crescentus with super‐resolution imaging based on fluorescent‐protein photoswitching and the natural polymerization/depolymerization dynamics of FtsZ associated with the Z‐ring. We quantify these dynamics and determine the FtsZ depolymerization time to be <100 ms. We image the Z‐ring in live and fixed C. crescentus cells at different stages of the cell cycle and find that the FtsZ superstructure is dynamic with the cell cycle, forming an open shape during the stalked stage and a dense focus during the pre‐divisional stage.

Bibliography

Biteen, J. S., Goley, E. D., Shapiro, L., & Moerner, W. E. (2012). ThreeâDimensional SuperâResolution Imaging of the Midplane Protein FtsZ in Live Caulobacter crescentus Cells Using Astigmatism. ChemPhysChem, 13(4), 1007â1012. Portico.

Authors 4

Julie S. Biteen (first)
Erin D. Goley (additional)
Lucy Shapiro (additional)
W. E. Moerner (additional)

References 33 Referenced 88

Dates

Type	When
Created	13 years, 7 months ago (Jan. 20, 2012, 2:47 a.m.)
Deposited	1 year, 11 months ago (Sept. 12, 2023, 8:24 p.m.)
Indexed	1 month, 1 week ago (July 20, 2025, 12:23 a.m.)
Issued	13 years, 7 months ago (Jan. 20, 2012)
Published	13 years, 7 months ago (Jan. 20, 2012)
Published Online	13 years, 7 months ago (Jan. 20, 2012)
Published Print	13 years, 5 months ago (March 1, 2012)

Funders 0

None

BibTeX

@article{Biteen_2012, title={Three‐Dimensional Super‐Resolution Imaging of the Midplane Protein FtsZ in Live Caulobacter crescentus Cells Using Astigmatism}, volume={13}, ISSN={1439-7641}, url={http://dx.doi.org/10.1002/cphc.201100686}, DOI={10.1002/cphc.201100686}, number={4}, journal={ChemPhysChem}, publisher={Wiley}, author={Biteen, Julie S. and Goley, Erin D. and Shapiro, Lucy and Moerner, W. E.}, year={2012}, month=jan, pages={1007–1012} }

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