DOI: 10.1002/cphc.200800537. Linking Phospholipase Mobility to Activity by Single‐Molecule Wide‐Field Microscopy

Linking Phospholipase Mobility to Activity by Single‐Molecule Wide‐Field Microscopy

10.1002/cphc.200800537

Crossref journal-article

Wiley

ChemPhysChem (311)

Abstract

AbstractMany of the biological processes taking place in cells are mediated by enzymatic reactions occurring in the cell membrane. Understanding interfacial enzymatic catalysis is therefore crucial to the understanding of cellular function. Unfortunately, a full picture of the overall mechanism of interfacial enzymatic catalysis, and particularly the important diffusion processes therein, remains unresolved. Herein we demonstrate that single‐molecule wide‐field fluorescence microscopy can yield important new information on these processes. We image phospholipase enzymes acting upon bilayers of their natural phospholipid substrate, tracking the diffusion of thousands of individual enzymes while simultaneously visualising local structural changes to the substrate layer. We study several enzyme types with different affinities and catalytic activities towards the substrate. Analysis of the trajectories of each enzyme type allows us successfully to correlate the mobility of phospholipase with its catalytic activity at the substrate. The methods introduced herein represent a promising new approach to the study of interfacial/heterogeneous catalysis systems.

Bibliography

Rocha, S., Hutchison, J. A., Peneva, K., Herrmann, A., MÃ¼llen, K., SkjÃ¸t, M., JÃ¸rgensen, C. I., Svendsen, A., De Schryver, F. C., Hofkens, J., & Ujiâi, H. (2009). Linking Phospholipase Mobility to Activity by SingleâMolecule WideâField Microscopy. ChemPhysChem, 10(1), 151â161. Portico.

Authors 11

Susana Rocha (first)
James A. Hutchison (additional)
Kalina Peneva (additional)
Andreas Herrmann (additional)
Klaus Müllen (additional)
Michael Skjøt (additional)
Christian I. Jørgensen (additional)
Allan Svendsen (additional)
Frans C. De Schryver (additional)
Johan Hofkens (additional)
Hiroshi Uji‐i (additional)

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Dates

Type	When
Created	16 years, 8 months ago (Dec. 4, 2008, 6:11 a.m.)
Deposited	1 year, 10 months ago (Sept. 28, 2023, 4:59 p.m.)
Indexed	1 year ago (Aug. 4, 2024, 5:45 a.m.)
Issued	16 years, 7 months ago (Jan. 7, 2009)
Published	16 years, 7 months ago (Jan. 7, 2009)
Published Online	16 years, 7 months ago (Jan. 7, 2009)
Published Print	16 years, 7 months ago (Jan. 12, 2009)

Funders 0

None

BibTeX

@article{Rocha_2009, title={Linking Phospholipase Mobility to Activity by Single‐Molecule Wide‐Field Microscopy}, volume={10}, ISSN={1439-7641}, url={http://dx.doi.org/10.1002/cphc.200800537}, DOI={10.1002/cphc.200800537}, number={1}, journal={ChemPhysChem}, publisher={Wiley}, author={Rocha, Susana and Hutchison, James A. and Peneva, Kalina and Herrmann, Andreas and Müllen, Klaus and Skjøt, Michael and Jørgensen, Christian I. and Svendsen, Allan and De Schryver, Frans C. and Hofkens, Johan and Uji‐i, Hiroshi}, year={2009}, month=jan, pages={151–161} }

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