Crossref journal-article
Wiley
ChemPhysChem (311)
Abstract

AbstractThis review presents an overview of the fluorescence detection and spectroscopy of single molecules (SMS) in liquids and on surfaces under ambient conditions. The various techniques of SMS, such as confocal epifluorescence detection and wide‐field imaging are presented and discussed, together with the different methods of data analysis such as fluorescence correlation spectroscopy and burst‐by‐burst analysis. Selected applications of the various techniques in physics, chemistry, and biology are described.

Bibliography

Böhmer, M., & Enderlein, J. (2003). Fluorescence Spectroscopy of Single Molecules under Ambient Conditions: Methodology and Technology. ChemPhysChem, 4(8), 792–808. Portico.

Authors 2
  1. Martin Böhmer (first)
  2. Jörg Enderlein (additional)
References 189 Referenced 87
  1. 10.1073/pnas.47.12.1981
  2. 10.1007/978-3-642-49204-4_32
  3. 10.1364/AO.15.002965
  4. 10.1364/AO.15.003135
  5. 10.1126/science.6823553
  6. 10.1016/0009-2614(90)85485-U
  7. 10.1002/9780470133552.ch1
  8. 10.1146/annurev.physchem.49.1.441
  9. 10.1007/978-3-0348-8817-2_13
  10. 10.1126/science.283.5408.1676
  11. 10.1021/cr980132z
  12. 10.1002/(SICI)1438-5171(200004)1:1<5::AID-SIMO5>3.0.CO;2-A
  13. 10.1021/ac022035i
  14. {'key': 'e_1_2_5_14_2', 'volume-title': 'Single Molecule Spectroscopy', 'year': '2001'} / Single Molecule Spectroscopy (2001)
  15. {'key': 'e_1_2_5_15_2', 'volume-title': 'Single Molecule Detection in Solution', 'year': '2002'} / Single Molecule Detection in Solution (2002)
  16. {'key': 'e_1_2_5_16_2', 'volume-title': 'Progress in Optics', 'author': 'Orrit M.'} / Progress in Optics by Orrit M.
  17. 10.1038/369040a0
  18. 10.1016/0301-0104(95)00391-6
  19. {'key': 'e_1_2_5_19_2', 'first-page': '191', 'volume-title': 'Single Molecule Optical Detection, Imaging and Spectroscopy', 'author': 'Trautman J. K.', 'year': '1997'} / Single Molecule Optical Detection, Imaging and Spectroscopy by Trautman J. K. (1997)
  20. 10.1021/jp971066s
  21. 10.1002/1361-6374(199803)6:1<43::AID-BIO6>3.0.CO;2-F
  22. 10.1002/(SICI)1097-0320(19990701)36:3<239::AID-CYTO14>3.0.CO;2-Y
  23. 10.1103/PhysRevLett.85.5312
  24. 10.1046/j.1365-2818.2001.00800.x
  25. 10.1063/1.481385
  26. 10.1021/jp973116z
  27. 10.1038/41048
  28. 10.1016/S0006-3495(02)75380-0
  29. 10.1016/S0006-3495(00)76764-6
  30. 10.1126/science.281.5385.2016
  31. 10.1038/90228
  32. 10.1002/1438-5171(200112)2:4<255::AID-SIMO255>3.0.CO;2-D
  33. 10.1073/pnas.162356799
  34. 10.1126/science.291.5501.103
  35. 10.1002/3527600809.ch12
  36. 10.1002/3527600809.ch5
  37. 10.1103/PhysRevLett.29.705
  38. 10.1002/bip.1974.360130102
  39. 10.1002/bip.1974.360130103
  40. 10.1002/3527600809.ch3
  41. 10.1016/S0006-3495(86)83709-2
  42. 10.1016/S0006-3495(86)83710-9
  43. 10.1016/S0006-3495(97)78833-7
  44. 10.1073/pnas.180317197
  45. 10.1016/S0009-2614(02)00044-1
  46. 10.1073/pnas.96.15.8461
  47. 10.1002/1438-5171(200110)2:3<177::AID-SIMO177>3.0.CO;2-4
  48. 10.1002/1438-5171(200012)1:4<279::AID-SIMO279>3.0.CO;2-S
  49. {'key': 'e_1_2_5_49_2', 'first-page': '857', 'volume': '44', 'author': 'Widengren J.', 'year': '1998', 'journal-title': 'Cell. Mol. Biol.'} / Cell. Mol. Biol. by Widengren J. (1998)
  50. 10.1021/bi980694c
  51. 10.1021/bi991236m
  52. 10.1016/S0301-4622(97)00061-6
  53. 10.1021/bi990366s
  54. 10.1016/S0006-3495(02)73935-0
  55. 10.1073/pnas.95.4.1416
  56. 10.1016/S0301-0104(99)00255-4
  57. 10.1073/pnas.95.23.13573
  58. 10.1073/pnas.97.22.11996
  59. 10.1073/pnas.96.18.10123
  60. 10.1016/S0301-0104(01)00486-4
  61. 10.1073/pnas.97.1.151
  62. 10.1039/b003268i
  63. 10.1016/S0006-3495(02)75621-X
  64. 10.1016/S0006-3495(01)75828-6
  65. 10.1021/jp000059s
  66. 10.1385/CBB:34:3:383
  67. 10.1021/bi0118512
  68. {'key': 'e_1_2_5_68_2', 'volume-title': 'Fluorescence Correlation Spectroscopy', 'year': '2001'} / Fluorescence Correlation Spectroscopy (2001)
  69. 10.1073/pnas.96.24.13756
  70. 10.1016/S0006-3495(99)76912-2
  71. 10.1006/meth.1999.0854
  72. 10.1016/S0006-3495(00)76523-4
  73. {'key': 'e_1_2_5_73_2', 'volume-title': 'Time‐correlated single photon counting', 'author': "O'Connor D. V.", 'year': '1984'} / Time‐correlated single photon counting by O'Connor D. V. (1984)
  74. 10.1364/JOSAB.9.001761
  75. 10.1063/1.109495
  76. 10.1063/1.1406926
  77. 10.1063/1.1144463
  78. 10.1016/0009-2614(96)00031-0
  79. 10.1016/S0009-2614(97)00409-0
  80. 10.1021/jp002358n
  81. 10.1007/s003400000405
  82. 10.1021/jp9708299
  83. 10.1016/S0168-1656(00)00412-0
  84. 10.1002/1438-5171(200112)2:4<251::AID-SIMO251>3.0.CO;2-T
  85. 10.1073/pnas.95.4.1556
  86. 10.1016/S0014-5793(98)01247-2
  87. {'key': 'e_1_2_5_87_2', 'first-page': '27', 'volume-title': 'Flow cytometry and sorting', 'author': 'Kachel V.', 'year': '1990'} / Flow cytometry and sorting by Kachel V. (1990)
  88. 10.1021/ac970545k
  89. {'key': 'e_1_2_5_89_2', 'volume-title': 'Topics in Fluorescence', 'author': 'Ambrose W. P.'} / Topics in Fluorescence by Ambrose W. P.
  90. 10.1002/1097-0320(20001101)41:3<203::AID-CYTO7>3.0.CO;2-2
  91. 10.1006/geno.2000.6218
  92. 10.1021/ac00095a005
  93. 10.1021/ac9611879
  94. 10.1016/S0009-2614(98)00096-7
  95. 10.1002/(SICI)1099-0801(199904)13:2<109::AID-BMC854>3.0.CO;2-R
  96. 10.1039/a901411j
  97. 10.1063/1.1149677
  98. 10.1021/ac9707242
  99. 10.1021/ac9703919
  100. 10.1073/pnas.96.1.11
  101. 10.1021/ac011076w
  102. 10.1039/an9941900349
  103. 10.1021/ac00114a013
  104. 10.1364/AO.34.003218
  105. 10.1021/ac970389h
  106. 10.1021/ac00114a023
  107. 10.1366/0003702971939136
  108. 10.1021/ac991428n
  109. 10.1002/3527600809.ch11
  110. 10.1021/ac00010a019
  111. 10.1021/ac00065a032
  112. 10.1364/OL.22.001265
  113. 10.1021/ac970093b
  114. 10.1021/ac971107g
  115. 10.1103/PhysRevLett.76.3931
  116. 10.1364/AO.38.001481
  117. 10.1021/ac980925l
  118. 10.1021/ac970990f
  119. 10.1002/1438-5171(200110)2:3<191::AID-SIMO191>3.0.CO;2-K
  120. 10.1002/(SICI)1097-0320(19990701)36:3<224::AID-CYTO12>3.0.CO;2-J
  121. 10.1021/ac991358k
  122. {'key': 'e_1_2_5_122_2', 'first-page': '259', 'volume': '41', 'author': 'Dapprich J.', 'year': '1995', 'journal-title': 'Exp. Tech. Phys.'} / Exp. Tech. Phys. by Dapprich J. (1995)
  123. 10.1126/science.272.5259.255
  124. 10.1073/pnas.93.13.6264
  125. 10.1103/PhysRevLett.77.3979
  126. 10.1063/1.118259
  127. 10.1002/1361-6374(199709)5:3<99::AID-BIO3>3.0.CO;2-W
  128. 10.1016/S0009-2614(97)00440-5
  129. 10.1103/PhysRevLett.80.2093
  130. 10.1021/jp990948j
  131. 10.1016/S0009-2614(97)00266-2
  132. 10.1103/PhysRevLett.84.1148
  133. 10.1038/35035032
  134. 10.1016/S0009-2614(01)00883-1
  135. 10.1103/PhysRevLett.85.4482
  136. 10.1046/j.1365-2818.2001.00795.x
  137. 10.1021/jp000213a
  138. 10.1016/S0022-2313(00)00185-X
  139. 10.1002/1438-5171(200009)1:3<215::AID-SIMO215>3.0.CO;2-S
  140. 10.1021/jp010365l
  141. 10.1073/pnas.94.20.10630
  142. 10.1016/S0006-3495(01)75722-0
  143. 10.1002/1438-5171(200208)3:4<183::AID-SIMO183>3.0.CO;2-8
  144. 10.1002/3527600809.ch6
  145. 10.1021/ja0012570
  146. 10.1002/1438-5171(200103)2:1<35::AID-SIMO35>3.0.CO;2-L
  147. 10.1126/science.282.5395.1877
  148. 10.1016/S0301-0104(99)00098-1
  149. 10.1002/1438-5171(200112)2:4<229::AID-SIMO229>3.0.CO;2-9
  150. 10.1021/jp981808x
  151. 10.1002/1521-3757(20011119)113:22<4323::AID-ANGE4323>3.0.CO;2-J
  152. 10.1002/1521-3773(20011119)40:22<4192::AID-ANIE4192>3.0.CO;2-D
  153. 10.1038/374555a0
  154. 10.1063/1.122276
  155. 10.1063/1.1138619
  156. 10.1002/1438-5171(200006)1:2<153::AID-SIMO153>3.0.CO;2-S
  157. 10.1002/1438-5171(200107)2:2<69::AID-SIMO69>3.0.CO;2-N
  158. 10.1016/S0006-3495(97)78139-6
  159. 10.1093/emboj/19.5.892
  160. 10.1002/3527600809.ch7
  161. 10.1038/307058a0
  162. 10.1006/bbrc.1997.6732
  163. 10.1016/S0006-3495(00)76843-3
  164. 10.1126/science.283.5405.1152
  165. 10.1016/S0006-3495(01)75925-5
  166. 10.1002/3527600809.ch9
  167. 10.1002/1438-5171(200211)3:5/6<267::AID-SIMO267>3.0.CO;2-X
  168. 10.1126/science.274.5289.966
  169. 10.1103/PhysRevLett.81.5322
  170. 10.1021/jp993364q
  171. 10.1021/jp9846330
  172. 10.1103/PhysRevLett.89.026101
  173. 10.1364/JOSAB.20.000554
  174. 10.1002/andp.19484370105
  175. {'key': 'e_1_2_5_174_2', 'volume-title': 'Resonance Energy Transfer', 'author': 'Andrews D. L.', 'year': '1999'} / Resonance Energy Transfer by Andrews D. L. (1999)
  176. 10.1021/jp010301a
  177. 10.1109/2944.577342
  178. 10.1073/pnas.96.7.3670
  179. 10.1016/S0301-0104(99)00149-4
  180. 10.1073/pnas.96.3.893
  181. 10.1016/S0301-0104(99)00127-5
  182. 10.1073/pnas.97.24.13021
  183. 10.1073/pnas.97.26.14241
  184. 10.1038/nature01060
  185. 10.1126/science.288.5473.2048
  186. 10.1016/S0006-3495(98)77931-7
  187. 10.1038/35004044
  188. 10.1038/78941
  189. 10.1002/3527600809.ch4
Dates
Type When
Created 22 years ago (Aug. 8, 2003, 9:18 a.m.)
Deposited 1 year, 10 months ago (Oct. 10, 2023, 12:35 p.m.)
Indexed 11 months, 3 weeks ago (Sept. 5, 2024, 11:06 a.m.)
Issued 22 years ago (Aug. 7, 2003)
Published 22 years ago (Aug. 7, 2003)
Published Online 22 years ago (Aug. 7, 2003)
Published Print 22 years ago (Aug. 18, 2003)
Funders 0

None

@article{B_hmer_2003, title={Fluorescence Spectroscopy of Single Molecules under Ambient Conditions: Methodology and Technology}, volume={4}, ISSN={1439-7641}, url={http://dx.doi.org/10.1002/cphc.200200565}, DOI={10.1002/cphc.200200565}, number={8}, journal={ChemPhysChem}, publisher={Wiley}, author={Böhmer, Martin and Enderlein, Jörg}, year={2003}, month=aug, pages={792–808} }