DOI: 10.1002/cne.903140410. Appearance and distribution of the 275 kD hair‐cell antigen during development of the avian inner ear

Appearance and distribution of the 275 kD hair‐cell antigen during development of the avian inner ear

10.1002/cne.903140410

Crossref journal-article

Wiley

Journal of Comparative Neurology (311)

Abstract

AbstractThe 275 kD hair‐cell antigen (HCA) is a protein that was originally identified using immunological techniques in the inner ears of early hatchling and adult chickens. The HCA is specifically associated with the apical surface of sensory hair cells; in the vestibular system the antigen is distributed over the entire stereocilia bundle, but in the auditory system it only extends a short distance up the shafts of the stereocilia. The objectives of this study were to ascertain when the HCA is first expressed during inner ear development, to compare the temporal and spatial patterns of HCA expression with those of neurite ingrowth, and to determine how the distribution of the antigen observed in the auditory system arises during development. Serial sections of otocysts from embryonic day (ED) 4 to ED7.5 (stages 24 to 32) were stained with a monoclonal antibody to the HCA and polyclonal antibodies to the neuron–glial cell adhesion molecule in order to analyse patterns of HCA expression and neurite ingrowth. Nerve fibres are first observed in the anterior pole of the otocyst at ED4.5 (stage 24), and in the evaginating basilar papilla by EDS (stage 26). The HCA first appears within the vestibular system in the anterior pole of the otocyst at EDS (stage 26), and within the auditory system in the distal end of the basilar papilla at ED6.5 (stage 29). Serial section analysis indicates that expression of the HCA is always limited to areas of the epithelium where nerve fibres are found, although the delay between the onset of innervation and the onset of HCA expression varies from one region of the otocyst to another. The growth of stereocilia bundles in the auditory system was studied from ED10 to 2 days after hatching in sections from the medial to distal regions of the basilar papilla double labelled with rhodamine phalloidin and monoclonal anti‐HCA, At ED12 the stereocilia bundles are 1.7 μm high and the staining observed with both phalloidin and the antibody extend to the same maximum height above the apical surface of the hair cell. The maximum height that anti‐HCA staining extends up the stereocilia bundle remains almost constant between ED12 and postnatal day 2, but between ED15 and ED18 the stereocilia bundle grows rapidly in height, with a membrane domain lacking the HCA forming at the distal ends of the stereocilia. The restricted distribution of the HCA observed on the apical surface of mature auditory hair cells in the basilar papilla is therefore generated during the final growth phase of the stereocilia bundle by the accumulation of HCA‐free membrane at the distal ends of the stereocilia.

Bibliography

Bartolami, S., Goodyear, R., & Richardson, G. (1991). Appearance and distribution of the 275 kD hairâcell antigen during development of the avian inner ear. Journal of Comparative Neurology, 314(4), 777â788. Portico.

Authors 3

S. Bartolami (first)
R. Goodyear (additional)
G. Richardson (additional)

References 36 Referenced 69

10.3109/00016487809124756
{'key': 'e_1_2_1_3_1', 'first-page': '181', 'article-title': 'Early differentiation of hair cells in the embryonic chick basilar papilla', 'volume': '273', 'author': 'Cotanche D. A.', 'year': '1983', 'journal-title': 'Arch. Otorhinolaryngol'} / Arch. Otorhinolaryngol / Early differentiation of hair cells in the embryonic chick basilar papilla by Cotanche D. A. (1983)
10.1016/0165-3806(84)90024-5
10.1002/cne.902880402
10.1002/aja.1001660406 / Am. J. Anat. / Contributions of placodal and neural crest cells to avian cranial peripheral ganglia by D'Amico Martel A. (1983)
10.1083/jcb.112.4.641
10.3109/00016488409130963
10.1083/jcb.75.2.339
Gil‐Loyzaga P.(1990)Maturation des elements sensorinerveux de la cochlée. Thesis Université des Sciences et Techniques du Languedoc (Montpellier II France).
{'key': 'e_1_2_1_11_1', 'first-page': '223', 'article-title': 'Trophic interaction between spiral neurons and the cochlear epithelium during in vitro development', 'volume': '10', 'author': 'Gil‐Loyzaga P.', 'year': '1987', 'journal-title': 'Assoc. Res. Otolaryngol. Abstr.'} / Assoc. Res. Otolaryngol. Abstr. / Trophic interaction between spiral neurons and the cochlear epithelium during in vitro development by Gil‐Loyzaga P. (1987)
10.1016/0012-1606(79)90247-1
10.1007/BF01181545
10.1083/jcb.98.5.1746
10.1002/jmor.1050880104
{'key': 'e_1_2_1_16_1', 'first-page': '277', 'article-title': 'Relations of symmetry in the developing embryo', 'volume': '36', 'author': 'Harrison R. G.', 'year': '1945', 'journal-title': 'Trans. Conn. Acad. Sci.'} / Trans. Conn. Acad. Sci. / Relations of symmetry in the developing embryo by Harrison R. G. (1945)
10.1007/BF01176994
10.1002/cne.902810110
10.1002/jmor.1051210302
10.1002/ar.1091910405
10.1002/ar.1091910406
10.3109/00016488309132723
10.1016/0012-1606(87)90223-5
10.1083/jcb.110.4.1055
{'key': 'e_1_2_1_25_1', 'first-page': '1', 'article-title': 'Development of the inner ear of the mouse: A radioautographic study of terminal mitoses', 'volume': '220', 'author': 'Ruben R. J.', 'year': '1967', 'journal-title': 'Acta Otolaryngol.'} / Acta Otolaryngol. / Development of the inner ear of the mouse: A radioautographic study of terminal mitoses by Ruben R. J. (1967)
10.1016/0012-1606(90)90251-D
10.1016/0012-1606(86)90048-5
10.1083/jcb.86.1.244
10.1016/0012-1606(86)90047-3
10.1083/jcb.106.2.355
10.1177/00034894760850S602 / Ann. Otol. Rhinol. Laryngol. / Effects of removal of the statoacoustic ganglion complex upon the growing otocyst by Van de Water T. R. (1976)
10.1007/BF00455590 / Z. Zellforsch. / Electron microscope study of the developing nerve terminals in the acoustic organs of the chick embryo by Vasquez‐Nin G. H. (1968)
10.1242/jeb.14.2.232
10.1016/0306-4522(85)90177-0
10.1016/0306-4522(85)90178-2 / Neuroscience / The growth of cochlear fibres and the formation of their synaptic endings in the avian inner ear: A study with the electron microscope by Whitehead M. C. (1985)
10.1002/jez.1400650303
10.1002/jez.1401130110

Dates

Type	When
Created	20 years, 7 months ago (Jan. 1, 2005, 5:51 p.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 11:21 p.m.)
Indexed	1 month, 2 weeks ago (July 7, 2025, 2:28 a.m.)
Issued	33 years, 8 months ago (Dec. 22, 1991)
Published	33 years, 8 months ago (Dec. 22, 1991)
Published Online	20 years, 10 months ago (Oct. 9, 2004)
Published Print	33 years, 8 months ago (Dec. 22, 1991)

Funders 0

None

BibTeX

@article{Bartolami_1991, title={Appearance and distribution of the 275 kD hair‐cell antigen during development of the avian inner ear}, volume={314}, ISSN={1096-9861}, url={http://dx.doi.org/10.1002/cne.903140410}, DOI={10.1002/cne.903140410}, number={4}, journal={Journal of Comparative Neurology}, publisher={Wiley}, author={Bartolami, S. and Goodyear, R. and Richardson, G.}, year={1991}, month=dec, pages={777–788} }

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