DOI: 10.1002/cne.902130106. Inhibitory and excitatory synapses in crayfish stretch receptor organs studied with direct rapid‐freezing and freeze‐substitution

Inhibitory and excitatory synapses in crayfish stretch receptor organs studied with direct rapid‐freezing and freeze‐substitution

10.1002/cne.902130106

Crossref journal-article

Wiley

Journal of Comparative Neurology (311)

Abstract

AbstractCrayfish abdominal stretch receptor organs are innervated by inhibitory (GABA) and excitatory (glutamate) synapses. Previous studies with aldehyde fixation showed that synaptic vesicles in the inhibitory synapse are flat and small, whereas those in the excitatory synapse are rounder and larger. We have reexamined these inhibitory and excitatory synapses by using direct rapid‐freezing and freeze‐substitution in order to preserve synaptic structure closer to its living state. Fine details of synaptic structure appear to be better preserved by this method. Synaptic vesicles in inhibitory as well as excitatory synapses are round, so the conventional flattened shape of vesicles in the inhibitory synapse must depend on some aspect of aldehyde processing. However, the average size of vesicles in the inhibitory synapse is significantly smaller than that of vesicles in the excitatory synapse, so synaptic vesicle size is regarded as having functional significance.

Bibliography

Nakajima, Y., & Reese, T. S. (1983). Inhibitory and excitatory synapses in crayfish stretch receptor organs studied with direct rapidâfreezing and freezeâsubstitution. Journal of Comparative Neurology, 213(1), 66â73. Portico.

Authors 2

Yasuko Nakajima (first)
Thomas S. Reese (additional)

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Dates

Type	When
Created	20 years, 7 months ago (Dec. 28, 2004, 3:02 p.m.)
Deposited	1 year, 10 months ago (Oct. 19, 2023, 3:40 p.m.)
Indexed	1 month, 3 weeks ago (July 7, 2025, 2:02 a.m.)
Issued	42 years, 7 months ago (Jan. 1, 1983)
Published	42 years, 7 months ago (Jan. 1, 1983)
Published Online	20 years, 10 months ago (Oct. 9, 2004)
Published Print	42 years, 7 months ago (Jan. 1, 1983)

Funders 0

None

BibTeX

@article{Nakajima_1983, title={Inhibitory and excitatory synapses in crayfish stretch receptor organs studied with direct rapid‐freezing and freeze‐substitution}, volume={213}, ISSN={1096-9861}, url={http://dx.doi.org/10.1002/cne.902130106}, DOI={10.1002/cne.902130106}, number={1}, journal={Journal of Comparative Neurology}, publisher={Wiley}, author={Nakajima, Yasuko and Reese, Thomas S.}, year={1983}, month=jan, pages={66–73} }

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