DOI: 10.1002/cne.901610311. An estimate of the number of cells arising by division in mouse cerebral hemispheres from age one to 12 months: An autoradiographic study of DNA synthesis

An estimate of the number of cells arising by division in mouse cerebral hemispheres from age one to 12 months: An autoradiographic study of DNA synthesis

10.1002/cne.901610311

Crossref journal-article

Wiley

Journal of Comparative Neurology (311)

Abstract

AbstractMale CBA strain mice aged 1, 2, 4, 6, 8 and 12 months were injected with 5 μCi of 6‐H3‐thymidine per gm body weight and killed two hours after the injection. Incorporation of the isotope, as a measure of DNA synthesis and cell division, was studied in the cerebral cortex and corpus callosum by light‐microscope autoradiography. DNA synthesis was found in a small number of nuclei of non‐neuronal cells, both of ecto‐ and mesodermal origin. The percentage of labeled cells (Labeling Index, or LI) decreased in both regions exponentially with age. On the base of measured LI values, the total number of cells arising from age one to 12 months was calculated. As the reference value the number of cells in one month‐old animals was taken. The calculated cell increment in the ectodermal population in the cortex amounted to 19.7–20.6% (average 20.2%) and to 196.0–425.0% (average 247.0%) in the corpus callosum (pairs of figures correspond to values calculated from LI values measured in sagittal and frontal sections). When all labeled cells (LC) were taken into consideration (of both ecto‐ and mesodermal origin) the corresponding cell increment ranged from 25.0–30.5% (average 26.8%) in the cortex and from 205.0–454.8% (average 263.0%) in the corpus callosum. The number of all newly arising cells in the whole hemisphere (including both cerebral cortex and corpus calosum) calculated from mean LI values of all LC in sagital and frontal sections amounted to 52.0%.It is suggested that the observed DNA synthesis and cell division, particularly in the corpus callosum and mesodermal cell groups of both regions studied, correspond mainly to renewal of cell populations. In the cerebral cortex accumulation of some newly arising cells in situ cannot be excluded.

Bibliography

MareÅ¡, V., Lodin, Z., & JÃlek, M. (1975). An estimate of the number of cells arising by division in mouse cerebral hemispheres from age one to 12 months: An autoradiographic study of DNA synthesis. Journal of Comparative Neurology, 161(3), 471â481. Portico.

Authors 3

Vladislav Mareš (first)
ZdeněK Lodin (additional)
Miloš Jílek (additional)

References 34 Referenced 25

10.1042/bj0980636 / Biochem. J. / The relationship between cellular nucleic acids in the developing rat cerebral cortex by Adams D. H. (1966)
{'key': 'e_1_2_1_3_1', 'first-page': '137', 'volume-title': 'Handbook of Neurochemistry', 'author': 'Altman J.', 'year': '1968'} / Handbook of Neurochemistry by Altman J. (1968)
10.1007/BF00519886
10.1093/geronj/23.3.289
{'key': 'e_1_2_1_6_1', 'first-page': '136', 'volume-title': 'Progress in Brain Research', 'author': 'Brizzee K. R.', 'year': '1964'} / Progress in Brain Research by Brizzee K. R. (1964)
{'key': 'e_1_2_1_7_1', 'first-page': '45', 'volume-title': 'Cellular and Molecular Renewal in the Mammalian Body', 'author': 'Buetov D. E.', 'year': '1971'} / Cellular and Molecular Renewal in the Mammalian Body by Buetov D. E. (1971)
10.1111/j.1471-4159.1972.tb05124.x
10.1016/B978-0-12-591650-9.50007-8
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{'key': 'e_1_2_1_12_1', 'first-page': '421', 'article-title': 'Cytokinetics of neonatal brain cell development in rats as studied by the “complete H3‐thymidine labelling” method', 'volume': '107', 'author': 'Haas R. J.', 'year': '1970', 'journal-title': 'J. Anat.'} / J. Anat. / Cytokinetics of neonatal brain cell development in rats as studied by the “complete H3‐thymidine labelling” method by Haas R. J. (1970)
{'key': 'e_1_2_1_13_1', 'first-page': '147', 'article-title': 'Evidence of mitosis in neuroglia as revealed by radioautography employing tritiated thymidine', 'volume': '19', 'author': 'Hain R. F.', 'year': '1960', 'journal-title': 'J. Neuropathol. Exp. Neurol.'} / J. Neuropathol. Exp. Neurol. / Evidence of mitosis in neuroglia as revealed by radioautography employing tritiated thymidine by Hain R. F. (1960)
10.1002/cne.901290304
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10.1016/0531-5565(72)90005-8
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{'key': 'e_1_2_1_18_1', 'first-page': '255', 'volume-title': 'Verhandl. d. Dtsch. Zool. Gesellsch', 'author': 'Korr H.', 'year': '1973'} / Verhandl. d. Dtsch. Zool. Gesellsch by Korr H. (1973)
10.1002/cne.901500205
10.1002/cne.901480206
10.1525/9780520331884-006 / Cellular Aspect of Neural Growth and Differentiation by Lapham L. W. (1971)
10.1002/cne.901490105
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{'key': 'e_1_2_1_26_1', 'first-page': '62', 'volume-title': 'Macormolecules and the Function of the Neuron', 'author': 'Mareš V.', 'year': '1968'} / Macormolecules and the Function of the Neuron by Mareš V. (1968)
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10.1007/BF00688831
10.1111/j.1471-4159.1973.tb00085.x
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{'key': 'e_1_2_1_33_1', 'first-page': '467', 'article-title': 'O vključeniji timidina H3 v kletki golovnovo mozga u vzroslych myšej v norme, pri proveždeniji mozga i vvedenije v nevo RNK', 'volume': '181', 'author': 'Ju. Reznikov K.', 'year': '1968', 'journal-title': 'Dokl. Akad. Nauk SSSR, Otd. Biokh.'} / Dokl. Akad. Nauk SSSR, Otd. Biokh. / O vključeniji timidina H3 v kletki golovnovo mozga u vzroslych myšej v norme, pri proveždeniji mozga i vvedenije v nevo RNK by Ju. Reznikov K. (1968)
10.1002/cne.901160306
10.1002/cne.901160307

Dates

Type	When
Created	20 years, 8 months ago (Jan. 1, 2005, 11:05 a.m.)
Deposited	1 year, 9 months ago (Nov. 12, 2023, 11:39 p.m.)
Indexed	1 year, 9 months ago (Nov. 13, 2023, 12:25 a.m.)
Issued	50 years, 3 months ago (June 1, 1975)
Published	50 years, 3 months ago (June 1, 1975)
Published Online	20 years, 10 months ago (Oct. 9, 2004)
Published Print	50 years, 3 months ago (June 1, 1975)

Funders 0

None

BibTeX

@article{Mare__1975, title={An estimate of the number of cells arising by division in mouse cerebral hemispheres from age one to 12 months: An autoradiographic study of DNA synthesis}, volume={161}, ISSN={1096-9861}, url={http://dx.doi.org/10.1002/cne.901610311}, DOI={10.1002/cne.901610311}, number={3}, journal={Journal of Comparative Neurology}, publisher={Wiley}, author={Mareš, Vladislav and Lodin, ZdeněK and Jílek, Miloš}, year={1975}, month=jun, pages={471–481} }

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