DOI: 10.1002/cm.970310306. Traction forces in locomoting cells

10.1002/cm.970310306

Crossref journal-article

Wiley

Cell Motility (311)

Abstract

AbstractA means of determining quantitative maps of the tractions exerted by locomoting cells on a substratum has been developed. This method is similar to the Harris silicone substratum assay [Harris et al., 1980: Science 208:177–179], but uses an improved non‐wrinkling film that deforms more predictably in response to traction forces. The method also utilizes a mathematical analysis of rubber deformation to produce the final map of the distribution of tractions. The resulting maps consistently showed that fish keratocytes exert a steady‐state “pinching” on the substratum, perpendicular to the cell's direction of locomotion. No significant rearward tractions were detected at or near the front edge of the cell. Likewise, no significant forward tractions associated with peeling of adhesions were found at the back of the cell. A second assay uses deflection of a lightly attached glass microneedle to measure the total force exerted by locomoting cells. Forces of approximately 4.5 × 10−3 dyn were required to “stall” locomoting keratocytes. The implications of these findings for cell movement are discussed.

Bibliography

Oliver, T., Jacobson, K., & Dembo, M. (1995). Traction forces in locomoting cells. Cell Motility, 31(3), 225â240. Portico.

Authors 3

Tim Oliver (first)
Ken Jacobson (additional)
Micah Dembo (additional)

References 28 Referenced 169

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{'key': 'e_1_2_1_26_1', 'first-page': '170a', 'article-title': 'Spatial distribution of dynamic traction forces applied by keratocytes to silicone elastic substrata', 'volume': '5', 'author': 'Park J.', 'year': '1994', 'journal-title': 'Mol. Biol. Cell Suppl. (abstract #987)'} / Mol. Biol. Cell Suppl. (abstract #987) / Spatial distribution of dynamic traction forces applied by keratocytes to silicone elastic substrata by Park J. (1994)
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Dates

Type	When
Created	20 years, 6 months ago (Feb. 23, 2005, 8:50 p.m.)
Deposited	1 year, 10 months ago (Oct. 26, 2023, 1:43 a.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:46 p.m.)
Issued	30 years, 7 months ago (Jan. 1, 1995)
Published	30 years, 7 months ago (Jan. 1, 1995)
Published Online	20 years, 6 months ago (Feb. 4, 2005)
Published Print	30 years, 7 months ago (Jan. 1, 1995)

Funders 0

None

BibTeX

@article{Oliver_1995, title={Traction forces in locomoting cells}, volume={31}, ISSN={0886-1544}, url={http://dx.doi.org/10.1002/cm.970310306}, DOI={10.1002/cm.970310306}, number={3}, journal={Cell Motility}, publisher={Wiley}, author={Oliver, Tim and Jacobson, Ken and Dembo, Micah}, year={1995}, month=jan, pages={225–240} }

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