DOI: 10.1002/cm.970290208. Localization of NuMA protein isoforms in the nuclear matrix of mammalian cells

Localization of NuMA protein isoforms in the nuclear matrix of mammalian cells

10.1002/cm.970290208

Crossref journal-article

Wiley

Cell Motility (311)

Abstract

AbstractUsing a monoclonal antibody 2D3 generated against a kinetochore‐enriched human chromosome preparation, we identified a high molecular mass protein with nuclear staining in interphase and polar staining of the pericentriolar region in the mitotic spindle. Initially termed centrophilin, this protein associates with the minus‐ends of spindle microtubules (MT) and appears to be important in spindle organization [Tousson et al., 1991: J. Cell Biol. 112:427–440]. Comparison of a partial cDNA sequence obtained for centrophilin with the full length cDNA sequence of nuclear mitotic apparatus protein (NuMA) [Compton et al., 1992: J. Cell Biol. 116:1395–1408; Yang et al., 1992: J. Cell Biol. 116:1303–1317] has indicated that NuMA and centrophilin are the same protein. Using a polyclonal NuMA antibody, we have provided further evidence that NuMA exists as iso‐forms as shown by peptide mapping and immunoblots. Sequential fractionation experiments along with immunofluorescence, immunoblotting, and EM immunogold labeling have demonstrated that NuMA isoforms are novel components of nuclear core filaments. Thus, NuMA, a long coiled‐coil protein, appears to have dual functions in interphase and mitosis during the cell cycle. In interphase, NuMA likely plays a structural role in the nucleoskeleton that may be important in nuclear organization and functions, whereas in mitosis, NuMA appears to be associated with spindle MT organization and chromosome positioning. © 1994 Wiley‐Liss, Inc.

Bibliography

Zeng, C., He, D., & Brinkley, B. R. (1994). Localization of NuMA protein isoforms in the nuclear matrix of mammalian cells. Cell Motility, 29(2), 167â176. Portico.

Authors 3

Changqing Zeng (first)
Dacheng He (additional)
B. R. Brinkley (additional)

References 42 Referenced 83

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{'key': 'e_1_2_1_25_1', 'first-page': '255', 'article-title': 'Identification of a minus end‐specific microtubule‐associated protein located at the mitotic poles in cultured mammalian cells', 'volume': '54', 'author': 'Maekawa T.', 'year': '1991', 'journal-title': 'Eur. J. Cell Biol.'} / Eur. J. Cell Biol. / Identification of a minus end‐specific microtubule‐associated protein located at the mitotic poles in cultured mammalian cells by Maekawa T. (1991)
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Dates

Type	When
Created	20 years, 6 months ago (Feb. 23, 2005, 8:54 p.m.)
Deposited	1 year, 10 months ago (Oct. 23, 2023, 8:34 p.m.)
Indexed	1 week, 5 days ago (Aug. 12, 2025, 5:42 p.m.)
Issued	31 years, 7 months ago (Jan. 1, 1994)
Published	31 years, 7 months ago (Jan. 1, 1994)
Published Online	20 years, 6 months ago (Feb. 4, 2005)
Published Print	31 years, 7 months ago (Jan. 1, 1994)

Funders 0

None

BibTeX

@article{Zeng_1994, title={Localization of NuMA protein isoforms in the nuclear matrix of mammalian cells}, volume={29}, ISSN={0886-1544}, url={http://dx.doi.org/10.1002/cm.970290208}, DOI={10.1002/cm.970290208}, number={2}, journal={Cell Motility}, publisher={Wiley}, author={Zeng, Changqing and He, Dacheng and Brinkley, B. R.}, year={1994}, month=jan, pages={167–176} }

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