DOI: 10.1002/cm.970280108. Structural and biochemical properties of kinesin heavy chain associated with rat brain mitochondria

Structural and biochemical properties of kinesin heavy chain associated with rat brain mitochondria

10.1002/cm.970280108

Crossref journal-article

Wiley

Cell Motility (311)

Abstract

AbstractKinesin, a mechanochemical enzyme that translocates membranous organelles, was initially identified and purified from soluble extracts from vertebrate brains. However, immunocytochemical and morphological approaches have demonstrated that kinesin could be associated to intracellular membranous organelles. We used an antibody raised against the head portion of the Drosophila kinesin heavy chain to reveal the presence of this protein in membranous organelles from rat brain. By using differential centrifugation and immunoblotting we observed a 116 kDa protein that crossreacts with this antibody in microsomes, synaptic vesicles, and mitochondria. This protein could be extracted from mitochondria with low salt concentrations or ATP. The 116 kDa solubilized protein has been identified as conventional kinesin based on limited sequence analysis. We also show that a polyclonal antibody raised against mitochondria‐associated kinesin recognizes soluble bovine brain kinesin. The soluble and mitochondrial membrane‐associated kinesins show a different isoform pattern. These results are consistent with the idea that kinesin exists as multiple isoforms that might be differentially distributed within the cell. In addition digitonin fractionation of mitochondria combined with KI extraction revealed that kinesin is a peripheral protein, preferentially located in a cholesterol‐free outer membrane domain; this domain has the features of contact points between the mitochondrial outer and inner membranes. The significance of these observations on the functional regulation of the mitochondria‐associated kinesin is discussed. © 1994 Wiley‐Liss, Inc.

Bibliography

Jellali, A., MetzâBoutigue, M., Surgucheva, I., Jancsik, V., Schwartz, C., Filliol, D., Gelfand, V. I., & Rendon, A. (1994). Structural and biochemical properties of kinesin heavy chain associated with rat brain mitochondria. Cell Motility, 28(1), 79â93. Portico.

Authors 8

Abdeljelil Jellali (first)
Marie‐Hélène Metz‐Boutigue (additional)
Irina Surgucheva (additional)
Veronika Jancsik (additional)
Christian Schwartz (additional)
Dominique Filliol (additional)
Vladimir I. Gelfand (additional)
Alvaro Rendon (additional)

References 64 Referenced 30

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Dates

Type	When
Created	20 years, 6 months ago (Feb. 23, 2005, 9:17 p.m.)
Deposited	1 year, 10 months ago (Oct. 23, 2023, 10:09 p.m.)
Indexed	1 month, 4 weeks ago (June 27, 2025, 6:35 a.m.)
Issued	31 years, 7 months ago (Jan. 1, 1994)
Published	31 years, 7 months ago (Jan. 1, 1994)
Published Online	20 years, 6 months ago (Feb. 4, 2005)
Published Print	31 years, 7 months ago (Jan. 1, 1994)

Funders 0

None

BibTeX

@article{Jellali_1994, title={Structural and biochemical properties of kinesin heavy chain associated with rat brain mitochondria}, volume={28}, ISSN={0886-1544}, url={http://dx.doi.org/10.1002/cm.970280108}, DOI={10.1002/cm.970280108}, number={1}, journal={Cell Motility}, publisher={Wiley}, author={Jellali, Abdeljelil and Metz‐Boutigue, Marie‐Hélène and Surgucheva, Irina and Jancsik, Veronika and Schwartz, Christian and Filliol, Dominique and Gelfand, Vladimir I. and Rendon, Alvaro}, year={1994}, month=jan, pages={79–93} }

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