DOI: 10.1002/cm.970190303. Pseudopodial activity at the active edge of migrating fibroblast is decreased after drug‐induced microtubule depolymerization

Pseudopodial activity at the active edge of migrating fibroblast is decreased after drug‐induced microtubule depolymerization

10.1002/cm.970190303

Crossref journal-article

Wiley

Cell Motility (311)

Abstract

AbstractIt is known that depolymerization of microtubules by colcemid or other similar drugs abolishes polarization of pseudopodial activity in migrating fibroblasts. In this work the effect of colcemid on the intensity of protrusion and retraction of lamellipodia at the active edges of human fibroblasts migrating into the wound was investigated with video‐enhanced contrast microscopy. To characterize the pseudopodial activity quantitatively the outlines of the active edges in the pairs of frames taken at adjacent 20‐sec intervals were compared and mean areas of protrusions and retractions per unit length of the perimeter of the edge were measured. The mean rates of protrusions and retractions were 4–6 times less in colcemid‐treated cells than in controls. Thus, microtubules depolymerized by colcemid, and/or intermediate filaments undergoing perinuclear collapse in the presence of this drug, are essential not only for the restriction of pseudopodial activity to one particular zone of the cell edge but also for the development of maximal activity in this zone.

Bibliography

Bershadsky, A. D., Vaisberg, E. A., & Vasiliev, J. M. (1991). Pseudopodial activity at the active edge of migrating fibroblast is decreased after drugâinduced microtubule depolymerization. Cell Motility, 19(3), 152â158. Portico.

Authors 3

Alexander D. Bershadsky (first)
Eugeni A. Vaisberg (additional)
Juri M. Vasiliev (additional)

References 21 Referenced 84

10.1016/0014-4827(70)90570-7
10.1002/cm.970010303
10.1073/pnas.79.1.123
10.1002/cm.970080308
10.1126/science.3277283
10.1126/science.6719108
{'key': 'e_1_2_1_8_1', 'first-page': '409', 'volume-title': 'Cell Adhesion and Motility', 'author': 'Dunn G. A.', 'year': '1980'} / Cell Adhesion and Motility by Dunn G. A. (1980)
10.1242/jcs.1987.Supplement_8.5
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{'key': 'e_1_2_1_11_1', 'first-page': '6366', 'article-title': 'Cytoplasmic microtubular motors', 'volume': '1', 'author': 'Gelfand V. I.', 'year': '1989', 'journal-title': 'Cu rr. Opin. Cell Biol.'} / Cu rr. Opin. Cell Biol. / Cytoplasmic microtubular motors by Gelfand V. I. (1989)
10.1002/cm.970160104
10.1002/cm.970060406
10.1242/jcs.92.4.621 / J. Cell Sci. / Intermediate filament collapse is an ATP‐dependent and actin‐dependent process by Hollenbeck P. J. (1989)
{'key': 'e_1_2_1_15_1', 'volume-title': 'Rank Correlation Methods', 'author': 'Kendall M. G.', 'year': '1962'} / Rank Correlation Methods by Kendall M. G. (1962)
10.1002/cm.970150202
10.1002/cm.970080210
{'key': 'e_1_2_1_18_1', 'first-page': '1', 'volume-title': 'Cell and Muscle Motility', 'author': 'Schliwa M.', 'year': '1984'} / Cell and Muscle Motility by Schliwa M. (1984)
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{'key': 'e_1_2_1_21_1', 'first-page': '279', 'volume-title': 'Cell Motility. Cold Spring Harbor Conf. Cell Proliferation', 'author': 'Vasiliev J. M.', 'year': '1976'} / Cell Motility. Cold Spring Harbor Conf. Cell Proliferation by Vasiliev J. M. (1976)
{'key': 'e_1_2_1_22_1', 'first-page': '625', 'article-title': 'Effect of colcemid on the locomotory behavior of fibroblasts', 'volume': '24', 'author': 'Vasiliev J. M.', 'year': '1970', 'journal-title': 'J. Embryol. Exp. Morphol.'} / J. Embryol. Exp. Morphol. / Effect of colcemid on the locomotory behavior of fibroblasts by Vasiliev J. M. (1970)

Dates

Type	When
Created	20 years, 6 months ago (Feb. 23, 2005, 9:59 p.m.)
Deposited	1 year, 10 months ago (Oct. 23, 2023, 12:26 a.m.)
Indexed	4 minutes ago (Sept. 2, 2025, 11:07 p.m.)
Issued	34 years, 8 months ago (Jan. 1, 1991)
Published	34 years, 8 months ago (Jan. 1, 1991)
Published Online	20 years, 6 months ago (Feb. 4, 2005)
Published Print	34 years, 8 months ago (Jan. 1, 1991)

Funders 0

None

BibTeX

@article{Bershadsky_1991, title={Pseudopodial activity at the active edge of migrating fibroblast is decreased after drug‐induced microtubule depolymerization}, volume={19}, ISSN={0886-1544}, url={http://dx.doi.org/10.1002/cm.970190303}, DOI={10.1002/cm.970190303}, number={3}, journal={Cell Motility}, publisher={Wiley}, author={Bershadsky, Alexander D. and Vaisberg, Eugeni A. and Vasiliev, Juri M.}, year={1991}, month=jan, pages={152–158} }

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