DOI: 10.1002/cm.970150106. Epidermal growth factor induces the accumulation of calpactin II on the cell surface during membrane ruffling

Epidermal growth factor induces the accumulation of calpactin II on the cell surface during membrane ruffling

10.1002/cm.970150106

Crossref journal-article

Wiley

Cell Motility (311)

Abstract

AbstractConfluent and proliferatively quiescent T51B rat liver epithelial cells provide a cellular model for the study of epidermal growth factor (EGF) effects in non‐neoplastic cells. Immunoreactive calpactin II, a well‐known substrate for EGF‐receptor kinase, was found predominantly in the cytosol, although a second im‐munoreactive pool was found in a Triton X‐100‐extractable membrane fraction. Stimulation with EGF resulted in a rapid and transient (2–;5 min) formation of ruffles at the cell surface and at the cell‐cell contacts. Both calpactin II and filamentous actin were found co‐localized at the membrane ruffles. Immunopre‐cipitations of membrane‐bound calpactin II from 32P‐labeled cells indicate a transient EGF‐dependent phosphorylation of calpactin II correlating with membrane ruffling. These results suggest a temporal (2–5 min) function for calpactin II at the plasma membrane during the EGF‐induced mitogenesis of T51B cells.

Bibliography

CamposâGonzalez, R., Kanemitsu, M., & Boynton, A. L. (1990). Epidermal growth factor induces the accumulation of calpactin II on the cell surface during membrane ruffling. Cell Motility, 15(1), 34â40. Portico.

Authors 3

Roberto Campos‐Gonzalez (first)
Martha Kanemitsu (additional)
Alton L. Boynton (additional)

References 39 Referenced 21

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Campos‐Gonzalez R. Kanemitsu M. andBoynton A. L.(1989): Tumor promoter‐dependent phosphorylation of a tritxon X‐100 extractable form of lipocortin I in T51B rat liver cells.Exp. Cell Res. in press. (10.1016/0014-4827(89)90328-5)
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Dates

Type	When
Created	20 years, 6 months ago (Feb. 23, 2005, 10:28 p.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 5:58 a.m.)
Indexed	1 year, 1 month ago (July 10, 2024, noon)
Issued	35 years, 8 months ago (Jan. 1, 1990)
Published	35 years, 8 months ago (Jan. 1, 1990)
Published Online	20 years, 6 months ago (Feb. 4, 2005)
Published Print	35 years, 8 months ago (Jan. 1, 1990)

Funders 0

None

BibTeX

@article{Campos_Gonzalez_1990, title={Epidermal growth factor induces the accumulation of calpactin II on the cell surface during membrane ruffling}, volume={15}, ISSN={0886-1544}, url={http://dx.doi.org/10.1002/cm.970150106}, DOI={10.1002/cm.970150106}, number={1}, journal={Cell Motility}, publisher={Wiley}, author={Campos‐Gonzalez, Roberto and Kanemitsu, Martha and Boynton, Alton L.}, year={1990}, month=jan, pages={34–40} }

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