DOI: 10.1002/cm.970130408. Dynamics of the endoplasmic reticulum in living non‐muscle and muscle cells

Dynamics of the endoplasmic reticulum in living non‐muscle and muscle cells

10.1002/cm.970130408

Crossref journal-article

Wiley

Cell Motility (311)

Abstract

AbstractThe dynamic changes of the endoplasmic reticulum (ER) in interphase and mitotic cells was detected by the vital fluorescent dye 3,3′‐dihexyloxacarbocyanine iodide. Two types of arrays characterize the continuous ER system in the non‐muscle PtK2 cell: (1) a lacy network of irregular polygons and (2) long strands of ER that are found aligned along stress fibers. In cross‐striated myotubes there was a periodic localization of fluorescence over each I‐band corresponding to the positions of the terminal cisternae of the sarcoplasmic reticulum (SR). In contrast to the arrangement in muscle cells, the aligment of the long strands of ER along stress fibers showed no strict periodicity that could be correlated with the sarcomeric units of the stress fibers. The ER and SR arrays seen in living cells were also detected in fixed cells stained with antibodies directed against proteins of the endoplasmic reticulum and sarcoplasmic reticulum, respectively. Observations of vitally stained PtK2 cells at 1 to 2 minute intervals using low light level video cameras and image processing techniques enabled us to see the polygonal ER units form and undergo changes in their shapes. During cell division, the ER, rhodamine 123‐stained mitochondria, and phagocytosed fluorescent beads were excluded from the mitotic spindle while soluble proteins were not. No obvious concentration or alignment of membranes could be found associated with the contractile proteins in the cleavage furrow. After completion of cell division there was a redeployment of the ER network in each daughter cell.

Bibliography

Sanger, J. M., Dome, J. S., Mittal, B., Somlyo, A. V., & Sanger, J. W. (1989). Dynamics of the endoplasmic reticulum in living nonâmuscle and muscle cells. Cell Motility, 13(4), 301â319. Portico.

Authors 5

Jean M. Sanger (first)
Jeffrey S. Dome (additional)
Balraj Mittal (additional)
Avril V. Somlyo (additional)
Joseph W. Sanger (additional)

References 65 Referenced 43

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Dates

Type	When
Created	20 years, 6 months ago (Feb. 23, 2005, 10:34 p.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 4:05 p.m.)
Indexed	1 year, 4 months ago (April 6, 2024, 1:14 a.m.)
Issued	36 years, 7 months ago (Jan. 1, 1989)
Published	36 years, 7 months ago (Jan. 1, 1989)
Published Online	20 years, 6 months ago (Feb. 4, 2005)
Published Print	36 years, 7 months ago (Jan. 1, 1989)

Funders 0

None

BibTeX

@article{Sanger_1989, title={Dynamics of the endoplasmic reticulum in living non‐muscle and muscle cells}, volume={13}, ISSN={0886-1544}, url={http://dx.doi.org/10.1002/cm.970130408}, DOI={10.1002/cm.970130408}, number={4}, journal={Cell Motility}, publisher={Wiley}, author={Sanger, Jean M. and Dome, Jeffrey S. and Mittal, Balraj and Somlyo, Avril V. and Sanger, Joseph W.}, year={1989}, month=jan, pages={301–319} }

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