Structural and chemical characterization of isolated centrosomes
10.1002/cm.970080305
Crossref journal-article
Wiley
Cell Motility (311)
Abstract

AbstractA procedure adapted from that described by Mitchison and Kirschner [Nature 312:232–237, 1984] was used to isolate centrosomes from human lymphoid cells. High yields of homogeneous centrosomes (60% of the theoretical total, assuming one centrosome per cell) were obtained. Centrosomes were isolated as pairs of centrioles, plus their associated pericentriolar material. Ultrastructural investigation revealed: 1) a link between both centrioles in a centrosome formed by the gathering in of a unique bundle of thin filaments surrounding each centriole; 2) a stereotypic organization of the pericentriolar material, including a rim of constant width at the proximal end of each centriole and a disc of nine satellite arms organized according to a ninefold symmetry at the distal end and; 3) an axial hub in the lumen of each centriole at the distal end surrounded by some ill‐defined material.The total protein content was 2 to 3 × 10−2 pg per isolated centrosome, a figure that suggests that the preparations were close to homogeneity. The protein composition was complex but specific, showing proteins ranging from 180 to 300 kD, one prominent band at 130 kD, and a group of proteins between 50 and 65 kD. Actin was also present in centrosome preparations.Functional studies demonstrated that the isolated centrosomes were competent to nucleate microtubules in vitro from purified tubulin in conditions in which spontaneous assembly could not occur. They were also very effective at inducing cleavage when microinjected into unfertilized Xenopus eggs.

Bibliography

Bornens, M., Paintrand, M., Berges, J., Marty, M., & Karsenti, E. (1987). Structural and chemical characterization of isolated centrosomes. Cell Motility, 8(3), 238–249. Portico.

Authors 5
  1. Michel Bornens (first)
  2. Michel Paintrand (additional)
  3. Josette Berges (additional)
  4. Marie‐Chantal Marty (additional)
  5. Eric Karsenti (additional)
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Dates
Type When
Created 20 years, 5 months ago (Feb. 23, 2005, 11:10 p.m.)
Deposited 1 year, 10 months ago (Oct. 21, 2023, 1:55 p.m.)
Indexed 1 month, 2 weeks ago (July 4, 2025, 12:14 a.m.)
Issued 38 years, 7 months ago (Jan. 1, 1987)
Published 38 years, 7 months ago (Jan. 1, 1987)
Published Online 20 years, 6 months ago (Feb. 4, 2005)
Published Print 38 years, 7 months ago (Jan. 1, 1987)
Funders 0

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@article{Bornens_1987, title={Structural and chemical characterization of isolated centrosomes}, volume={8}, ISSN={0886-1544}, url={http://dx.doi.org/10.1002/cm.970080305}, DOI={10.1002/cm.970080305}, number={3}, journal={Cell Motility}, publisher={Wiley}, author={Bornens, Michel and Paintrand, Michel and Berges, Josette and Marty, Marie‐Chantal and Karsenti, Eric}, year={1987}, month=jan, pages={238–249} }