DOI: 10.1002/cm.970010202. Distribution of tubulin and actin in neurites and growth cones of differentiating nerve cells

Distribution of tubulin and actin in neurites and growth cones of differentiating nerve cells

10.1002/cm.970010202

Crossref journal-article

Wiley

Cell Motility (311)

Abstract

AbstractEmbryonic chick nerve cells, from dissociated dorsal root ganglia, were cultured on polylysine substrata and examined for tubulin and actin distribution by indirect immunofluorescence.Antibodies generated against chick brain tubulin produced specific fluorescence in growth cones, neurites, and cell bodies without revealing distribution differences or substructure in the nerve cells. However, at reduced antitubulin concentrations, differences were resolved. Tubulin fluorescence remained uniform and intense in neurites and cell bodies, but exhibited reduced intensity and patterning in growth cones. Nonneuronal cells in the reduced intensity and patterning in growth cones. Nonneuronal cells in the cultures served as controls for typical cytoplasmic tubulin fluorescence distribution. Straining controls demonstrated that fluorescence resulted from tubulin‐antitubulin binding.Analogous studies, using antibodies generated against chick brain actin, demonstrated distribution differences at reduced antiactin concentrations, including “hot spots” of intense fluorescence in growth cones and a paucity of fluorescence in neurites.

Bibliography

Spooner, B. S., & Holladay, C. R. (1981). Distribution of tubulin and actin in neurites and growth cones of differentiating nerve cells. Cell Motility, 1(2), 167â178. Portico.

Authors 2

Brian S. Spooner (first)
Carter R. Holladay (additional)

References 26 Referenced 36

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{'key': 'e_1_2_1_7_1', 'first-page': '382', 'article-title': 'Distribution of actin and tubulin in neuroblastoma cells', 'volume': '15', 'author': 'Isenberg G.', 'year': '1977', 'journal-title': 'Eur. J. Cell Biol.'} / Eur. J. Cell Biol. / Distribution of actin and tubulin in neuroblastoma cells by Isenberg G. (1977)
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{'key': 'e_1_2_1_22_1', 'first-page': '325a', 'article-title': 'Structural differences in actins revealed by radioimmunoassay', 'volume': '83', 'author': 'Spooner B. S.', 'year': '1979', 'journal-title': 'J. Cell Biol.'} / J. Cell Biol. / Structural differences in actins revealed by radioimmunoassay by Spooner B. S. (1979)
10.1073/pnas.72.2.459
{'key': 'e_1_2_1_24_1', 'first-page': '53', 'article-title': 'Surface movements, microfilaments, and cell locomotion', 'volume': '14', 'author': 'Wessells N. K.', 'year': '1973', 'journal-title': 'Ciba Sympos.'} / Ciba Sympos. / Surface movements, microfilaments, and cell locomotion by Wessells N. K. (1973)
10.1016/0040-8166(74)90014-7
10.1073/pnas.66.4.1206
10.1083/jcb.49.3.614

Dates

Type	When
Created	20 years, 6 months ago (Feb. 23, 2005, 11:49 p.m.)
Deposited	1 year, 9 months ago (Nov. 12, 2023, 5:28 a.m.)
Indexed	1 year, 1 month ago (July 15, 2024, 7:13 a.m.)
Issued	44 years, 8 months ago (Jan. 1, 1981)
Published	44 years, 8 months ago (Jan. 1, 1981)
Published Online	20 years, 7 months ago (Feb. 4, 2005)
Published Print	44 years, 8 months ago (Jan. 1, 1981)

Funders 0

None

BibTeX

@article{Spooner_1981, title={Distribution of tubulin and actin in neurites and growth cones of differentiating nerve cells}, volume={1}, ISSN={0271-6585}, url={http://dx.doi.org/10.1002/cm.970010202}, DOI={10.1002/cm.970010202}, number={2}, journal={Cell Motility}, publisher={Wiley}, author={Spooner, Brian S. and Holladay, Carter R.}, year={1981}, month=jan, pages={167–178} }

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