DOI: 10.1002/cm.20237. Movement of stress fibers away from focal adhesions identifies focal adhesions as sites of stress fiber assembly in stationary cells

Movement of stress fibers away from focal adhesions identifies focal adhesions as sites of stress fiber assembly in stationary cells

10.1002/cm.20237

Crossref journal-article

Wiley

Cell Motility (311)

Abstract

AbstractForce generated in contractile actin filament bundles (stress fibers—SFs) is transmitted to the extracellular matrix (ECM) via linker proteins and transmembrane integrins at focal adhesions (FAs). Though it has long been known that actin is rapidly exchanged in FAs, the connection between SFs and FAs has not been studied in detail. We introduced fiduciary marks on SFs by expressing GFP‐palladin or GFP‐α‐actinin‐1, which are both FA and dense body proteins, and by pattern bleaching of GFP‐actin. Following fiduciary marks on SFs over time by time‐lapse fluorescence microscopy, we detected assembly of SFs at FAs in stationary cells resulting in movement of SFs away from FAs with a velocity of 0.2–0.4 μm/min. Visualization of FAs in GFP‐palladin/DsRed‐paxillin double transfected cells showed that SF elongation was not accompanied by a change in FA length. SF elongation at FAs depended on actin polymerization and force as demonstrated by inhibitors of actin polymerization (cytochalasin D, jasplakinolide) and inhibitors of myosin‐dependent contraction (blebbistatin, Y‐27632), respectively. Our finding of SF assembly at FAs has important implications for SF formation, force transmission, and tension distribution within the actin cytoskeletal network of stationary cells. Cell Motil. Cytoskeleton 2007. © 2007 Wiley‐Liss, Inc.

Bibliography

Endlich, N., Otey, C. A., Kriz, W., & Endlich, K. (2007). Movement of stress fibers away from focal adhesions identifies focal adhesions as sites of stress fiber assembly in stationary cells. Cell Motility, 64(12), 966â976. Portico.

Authors 4

Nicole Endlich (first)
Carol A. Otey (additional)
Wilhelm Kriz (additional)
Karlhans Endlich (additional)

References 36 Referenced 54

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Dates

Type	When
Created	17 years, 11 months ago (Sept. 14, 2007, 6:48 p.m.)
Deposited	1 year, 9 months ago (Nov. 15, 2023, 1:54 a.m.)
Indexed	2 weeks, 6 days ago (Aug. 12, 2025, 5:27 p.m.)
Issued	17 years, 11 months ago (Sept. 14, 2007)
Published	17 years, 11 months ago (Sept. 14, 2007)
Published Online	17 years, 11 months ago (Sept. 14, 2007)
Published Print	17 years, 9 months ago (Dec. 1, 2007)

Funders 0

None

BibTeX

@article{Endlich_2007, title={Movement of stress fibers away from focal adhesions identifies focal adhesions as sites of stress fiber assembly in stationary cells}, volume={64}, ISSN={0886-1544}, url={http://dx.doi.org/10.1002/cm.20237}, DOI={10.1002/cm.20237}, number={12}, journal={Cell Motility}, publisher={Wiley}, author={Endlich, Nicole and Otey, Carol A. and Kriz, Wilhelm and Endlich, Karlhans}, year={2007}, month=sep, pages={966–976} }

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