DOI: 10.1002/cm.10173. Palladin is a novel binding partner for Ena/VASP family members

Palladin is a novel binding partner for Ena/VASP family members

10.1002/cm.10173

Crossref journal-article

Wiley

Cell Motility (311)

Abstract

AbstractPalladin is an actin‐associated protein that contains proline‐rich motifs within its amino‐terminal sequence that are similar to motifs found in zyxin, vinculin, and the Listeria protein ActA. These motifs are known to be potential binding sites for the Vasodilator‐Stimulated Phosphoprotein (VASP). Here, we demonstrate that palladin is an additional direct binding partner for VASP, by using co‐immunoprecipitation and blot overlay techniques with both endogenous palladin and recombinant myc‐tagged palladin. These results show that VASP binds to full‐length palladin and also to the amino‐terminal half of palladin, where the polyproline motifs are located. Using a synthetic peptide array, two discrete binding sites for VASP were identified within palladin's proline‐rich amino‐terminal domain. Using double‐label immunofluorescence staining of fully‐spread and actively‐spreading fibroblasts, the extent of co‐localization of palladin and VASP was explored. These proteins were found to strongly co‐localize along stress fibers, and partially co‐localize in focal adhesions, lamellipodia, and focal complexes. These results suggest that the recently described actin‐associated protein palladin may play an important role in recruiting VASP to sites of actin filament growth, anchorage, and crosslinking. Cell Motil. Cytoskeleton 58:17–29, 2004. © 2004 Wiley‐Liss, Inc.

Bibliography

Boukhelifa, M., Parast, M. M., Bear, J. E., Gertler, F. B., & Otey, C. A. (2004). Palladin is a novel binding partner for Ena/VASP family members. Cell Motility, 58(1), 17â29. Portico.

Authors 5

Malika Boukhelifa (first)
Mana M. Parast (additional)
James E. Bear (additional)
Frank B. Gertler (additional)
Carol A. Otey (additional)

References 64 Referenced 71

Dates

Type	When
Created	21 years, 6 months ago (Feb. 20, 2004, 9 a.m.)
Deposited	1 year, 9 months ago (Nov. 17, 2023, 11:46 a.m.)
Indexed	4 months, 2 weeks ago (April 22, 2025, 4:09 a.m.)
Issued	21 years, 6 months ago (Feb. 12, 2004)
Published	21 years, 6 months ago (Feb. 12, 2004)
Published Online	21 years, 6 months ago (Feb. 12, 2004)
Published Print	21 years, 4 months ago (May 1, 2004)

Funders 0

None

BibTeX

@article{Boukhelifa_2004, title={Palladin is a novel binding partner for Ena/VASP family members}, volume={58}, ISSN={0886-1544}, url={http://dx.doi.org/10.1002/cm.10173}, DOI={10.1002/cm.10173}, number={1}, journal={Cell Motility}, publisher={Wiley}, author={Boukhelifa, Malika and Parast, Mana M. and Bear, James E. and Gertler, Frank B. and Otey, Carol A.}, year={2004}, month=feb, pages={17–29} }

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