DOI: 10.1002/ceat.200800082. A Review of the Thermodynamics of Protein Association to Ligands, Protein Adsorption, and Adsorption Isotherms

A Review of the Thermodynamics of Protein Association to Ligands, Protein Adsorption, and Adsorption Isotherms

10.1002/ceat.200800082

Crossref journal-article

Wiley

Chemical Engineering & Technology (311)

Abstract

AbstractThe application of thermodynamic models in the development of chromatographic separation processes is discussed. The paper analyses the thermodynamic principles of protein adsorption. It can be modeled either as a reversible association between the adsorbate and the ligands or as a steady‐state process where the rate of adsorption is equal to the rate of desorption. The analysis includes the competitive Langmuir isotherm and the exponentially modified Langmuir isotherm. If the adsorbate binds to one ligand only, the different approaches become identical. When the adsorbate acts as a ligand, dimerization takes place and will give rise to a sigmoid isotherm. A model that accounts for dimerization is discussed and a sample calculation shows the behavior of this isotherm. Insulin is known to have a concave isotherm at low concentrations. The calculation of the standard Gibbs energy change of adsorption is discussed. Hydrophobic and reversed phase chromatography are useful techniques for measuring solute activity coefficients at infinite dilution.

Bibliography

Mollerup, J. M. (2008). A Review of the Thermodynamics of Protein Association to Ligands, Protein Adsorption, and Adsorption Isotherms. Chemical Engineering & Technology, 31(6), 864â874. Portico.

Authors 1

J. M. Mollerup (first)

References 25 Referenced 103

G. Guiochon A. Felinger D. G. G. Shirazi A. M. Katti Fundamentals of Preparative and Nonlinear Chromatography 2nd ed. Academic Press Amsterdam2006. (10.1016/B978-012370537-2/50030-8)
J. M. Smith H. C. van Ness Introduction to Chemical Engineering Thermodynamics 3rd ed. McGraw‐Hill New York1975.
P. W. Atkins Physical Chemistry 5th ed. Oxford University Press Oxford1994.
10.1016/S1570-0232(03)00127-2
10.1351/pac199365040819
10.1016/0003-9861(77)90434-9
10.1016/j.jbiotec.2007.05.036
10.1002/aic.690381212
10.1016/0301-4622(94)00006-9
10.1016/j.fluid.2007.07.047
10.1016/j.chroma.2007.08.059
L. Pedersen Ph.D. Thesis Technical University of Denmark2002.
J. Brange Galenics of Insulin Springer‐Verlag Berlin1987. (10.1007/978-3-662-02526-0)
S. F. Frederiksen Novo Nordisk A/S personal communication.
10.1016/S0021-9673(02)02047-2
10.1016/S0021-9673(01)88960-3
10.1002/(SICI)1097-0290(20000620)68:6<672::AID-BIT11>3.0.CO;2-Q
10.1021/bp049712
10.1016/j.fluid.2005.12.037
10.1016/j.chroma.2007.11.003
10.1016/j.chroma.2007.09.031
X. Li A. M. Hupp V. L. McGuffin inAdvances in Chromatography(Eds: E. Grushka N. Grinberg) Vol. 45 CRC Press Boca Raton FL2006.
10.1016/j.chroma.2007.05.009
10.1016/j.chroma.2005.04.005
10.1021/ie9507437

Dates

Type	When
Created	17 years, 3 months ago (May 27, 2008, 6:49 a.m.)
Deposited	1 year, 11 months ago (Sept. 12, 2023, 1:29 p.m.)
Indexed	2 months, 2 weeks ago (June 17, 2025, 2:25 a.m.)
Issued	17 years, 3 months ago (May 27, 2008)
Published	17 years, 3 months ago (May 27, 2008)
Published Online	17 years, 3 months ago (May 27, 2008)
Published Print	17 years, 3 months ago (June 1, 2008)

Funders 0

None

BibTeX

@article{Mollerup_2008, title={A Review of the Thermodynamics of Protein Association to Ligands, Protein Adsorption, and Adsorption Isotherms}, volume={31}, ISSN={1521-4125}, url={http://dx.doi.org/10.1002/ceat.200800082}, DOI={10.1002/ceat.200800082}, number={6}, journal={Chemical Engineering & Technology}, publisher={Wiley}, author={Mollerup, J. M.}, year={2008}, month=may, pages={864–874} }

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