DOI: 10.1002/bmc.203. Synthesis and analysis of aminochromes by HPLC‐photodiode array. Adrenochrome evaluation in rat blood

Synthesis and analysis of aminochromes by HPLC‐photodiode array. Adrenochrome evaluation in rat blood

10.1002/bmc.203

Crossref journal-article

Wiley

Biomedical Chromatography (311)

Abstract

AbstractThe catecholamine oxidation process induces cardiotoxicity and neurotoxicity. Catecholamines can oxidize to aminochromes through autoxidation or by enzymatic or non‐enzymatic catalysis. Although some toxic effects seem to be related to the formation of aminochromes there is still scarce information concerning the identification and evaluation of these compounds in in vivo models. In this study five catecholamines were oxidized to their respective aminochromes: adrenaline/adrenochrome; noradrenaline/noradrenochrome; dopa/dopachrome; dopamine/dopaminochrome; and isoproterenol/isoprenochrome. The evaluation of the catecholamines oxidation profile was performed by HPLC with photodiode array detection and using either enzymatic (tyrosinase) or non‐enzymatic [Ag2O, CuSO4, NaIO4 and K3Fe(CN)6] catalytic systems. The NaIO4 was found to be the most efficient oxidant of catecholamines. An isocratic reverse‐phase HPLC method was developed to analyse each pair of catecholamine–aminochrome. The analytical system was then applied to the detection of adrenochrome in rat blood at 490 nm. Thus, adrenochrome was administered i.p. to rats and its concentration in whole blood was monitored after 5, 15 and 25 min. Blood treatment for adrenochrome evaluation consists of an acidification for protein precipitation followed by a rapid neutralization. The results showed a rapid decrease of adrenochrome concentration in blood after its administration. The adrenochrome present in blood was characterized by UV and tandem mass spectrometry. Copyright © 2002 John Wiley & Sons, Ltd.

Bibliography

RemiÃ£o, F., Milhazes, N., Borges, F., Carvalho, F., Bastos, M. L., LemosâAmado, F., Domingues, P., & FerrerâCorreia, A. (2002). Synthesis and analysis of aminochromes by HPLCâphotodiode array. Adrenochrome evaluation in rat blood. Biomedical Chromatography, 17(1), 6â13. Portico.

Authors 8

Fernando Remião (first)
Nuno Milhazes (additional)
Fernanda Borges (additional)
Félix Carvalho (additional)
Maria L. Bastos (additional)
Francisco Lemos‐Amado (additional)
Pedro Domingues (additional)
A. Ferrer‐Correia (additional)

References 30 Referenced 23

10.1016/S0002-8703(81)80001-4
10.1002/jat.793
10.1016/0378-4274(89)90180-X
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10.1007/BF00421086
10.1007/BF00120492
{'key': 'e_1_2_1_8_1', 'first-page': '269', 'volume-title': 'Cardiovascular Toxicity', 'author': 'Dhalla NS', 'year': '2001'} / Cardiovascular Toxicity by Dhalla NS (2001)
10.1006/jmcc.1996.0362
10.1007/BF02170909
{'key': 'e_1_2_1_11_1', 'first-page': '122', 'article-title': 'Adrenochrome uptake and subcellular distribution in the isolated perfused rat heart', 'volume': '1', 'author': 'Fliegel L', 'year': '1985', 'journal-title': 'Canadian Journal of Cardiology'} / Canadian Journal of Cardiology / Adrenochrome uptake and subcellular distribution in the isolated perfused rat heart by Fliegel L (1985)
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10.1016/S0891-5849(98)00027-6
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10.1006/abio.1996.9997
10.1002/rcm.498
10.1006/bbrc.2001.4907
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10.1016/S0009-2797(99)00124-6
10.1016/0006-2952(62)90229-0
10.1016/0167-4838(95)00226-X
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10.1080/14756369909030340
10.1385/CT:1:3:195
10.1016/S0891-5849(01)00758-4
{'key': 'e_1_2_1_27_1', 'first-page': '443', 'article-title': 'An infarct‐like myocardial lesion and other toxic manifestations produced by isoproterenol in the rat', 'volume': '67', 'author': 'Rona G', 'year': '1959', 'journal-title': 'Archives of Pathology'} / Archives of Pathology / An infarct‐like myocardial lesion and other toxic manifestations produced by isoproterenol in the rat by Rona G (1959)
10.1097/00005344-199424001-00004
{'key': 'e_1_2_1_29_1', 'first-page': '167', 'article-title': 'Myocardial cell damage and cardiovascular changes due to I.V. infusion of adrenochrome in rats', 'volume': '63', 'author': 'Singal PK', 'year': '1982', 'journal-title': 'British Journal of Experimental Pathology'} / British Journal of Experimental Pathology / Myocardial cell damage and cardiovascular changes due to I.V. infusion of adrenochrome in rats by Singal PK (1982)
10.1016/S0304-4165(97)00131-1
10.1006/abbi.2000.2234

Dates

Type	When
Created	22 years, 6 months ago (Feb. 12, 2003, 3:05 p.m.)
Deposited	1 year, 9 months ago (Nov. 17, 2023, 2:37 p.m.)
Indexed	1 year, 2 months ago (July 2, 2024, 8:36 a.m.)
Issued	22 years, 8 months ago (Dec. 24, 2002)
Published	22 years, 8 months ago (Dec. 24, 2002)
Published Online	22 years, 8 months ago (Dec. 24, 2002)
Published Print	22 years, 8 months ago (Jan. 1, 2003)

Funders 2

FEDER European Comminity Funding
Awards1
1. POCT1/36099/FCB/2000
Project PRAXIS
Awards1
1. XXI/BD/18520/98

BibTeX

@article{Remi_o_2002, title={Synthesis and analysis of aminochromes by HPLC‐photodiode array. Adrenochrome evaluation in rat blood}, volume={17}, ISSN={1099-0801}, url={http://dx.doi.org/10.1002/bmc.203}, DOI={10.1002/bmc.203}, number={1}, journal={Biomedical Chromatography}, publisher={Wiley}, author={Remião, Fernando and Milhazes, Nuno and Borges, Fernanda and Carvalho, Félix and Bastos, Maria L. and Lemos‐Amado, Francisco and Domingues, Pedro and Ferrer‐Correia, A.}, year={2002}, month=dec, pages={6–13} }

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