DOI: 10.1002/bit.260400305. Cell cycle model for growth rate and death rate in continuous suspension hybridoma cultures

Cell cycle model for growth rate and death rate in continuous suspension hybridoma cultures

10.1002/bit.260400305

Crossref journal-article

Wiley

Biotechnology and Bioengineering (311)

Abstract

AbstractAs a result of recent advances in flow cytometry, renewed interest is shown in modeling the kinetic behavior of cells in culture on the basis of cell cycle parameters. An important but often overlooked kinetic variable in hybridoma cultures is the cell death rate. Not only the overall cell growth but also the kinetics of nutrient metabolism and monoclonal antibody production have been shown to depend on the cell death rate in continuous suspension hybridoma cultures. The present study shows that the death rate in hybridoma cultures is proportional to the fraction of cells arrested in the G1 phase of the cell cycle. The steady‐state cell age distributions in the various phases of the division cycle have been calculated analytically. A simple mathematical model has been used to produce the profiles of the cycling and arrested cell fractions with respect to the dilution rate. The calculated steady‐state growth rate, death rate, and viability profiles are shown to be in agreement with recently published experimental data from continuous suspension hybridoma cultures. © 1992 John Wiley & Sons, Inc.

Bibliography

Linardos, T. I., Kalogerakis, N., & Behie, L. A. (1992). Cell cycle model for growth rate and death rate in continuous suspension hybridoma cultures. Biotechnology and Bioengineering, 40(3), 359â368. Portico.

Authors 3

Theodor I. Linardos (first)
Nicolas Kalogerakis (additional)
Leo A. Behie (additional)

References 42 Referenced 42

{'key': 'e_1_2_1_2_2', 'first-page': '85', 'volume-title': 'Biomathematics and cell kinetics', 'author': 'Alberghina L.', 'year': '1978'} / Biomathematics and cell kinetics by Alberghina L. (1978)
{'key': 'e_1_2_1_3_2', 'volume-title': 'Molecular biology of the cell', 'author': 'Alberts B.', 'year': '1983'} / Molecular biology of the cell by Alberts B. (1983)
10.1177/28.6.6156196
{'key': 'e_1_2_1_5_2', 'volume-title': 'Statistical procedures for engineering, management, and science', 'author': 'Blank L.', 'year': '1980'} / Statistical procedures for engineering, management, and science by Blank L. (1980)
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{'key': 'e_1_2_1_9_2', 'first-page': '321', 'article-title': 'On the existence of a G\n 0‐phase in the cell cycle', 'volume': '3', 'author': 'Burns F. J.', 'year': '1970', 'journal-title': 'Cell Tissue Kinet.'} / Cell Tissue Kinet. / On the existence of a G 0‐phase in the cell cycle by Burns F. J. (1970)
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10.1002/aja.1001170106
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10.1038/248241a0
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{'key': 'e_1_2_1_26_2', 'volume-title': 'Reproduction of eukaryotic cells', 'author': 'Prescott D. M.', 'year': '1976'} / Reproduction of eukaryotic cells by Prescott D. M. (1976)
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10.1016/0014-4827(78)90068-X

Dates

Type	When
Created	20 years, 8 months ago (Dec. 29, 2004, 11:49 p.m.)
Deposited	1 year, 10 months ago (Oct. 23, 2023, 9:14 p.m.)
Indexed	1 month, 3 weeks ago (July 14, 2025, 11:48 p.m.)
Issued	33 years, 2 months ago (July 1, 1992)
Published	33 years, 2 months ago (July 1, 1992)
Published Online	21 years, 6 months ago (Feb. 19, 2004)
Published Print	33 years, 2 months ago (July 1, 1992)

Funders 0

None

BibTeX

@article{Linardos_1992, title={Cell cycle model for growth rate and death rate in continuous suspension hybridoma cultures}, volume={40}, ISSN={1097-0290}, url={http://dx.doi.org/10.1002/bit.260400305}, DOI={10.1002/bit.260400305}, number={3}, journal={Biotechnology and Bioengineering}, publisher={Wiley}, author={Linardos, Theodor I. and Kalogerakis, Nicolas and Behie, Leo A.}, year={1992}, month=jul, pages={359–368} }

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