DOI: 10.1002/bit.260350110. Encapsulation of mammalian cell with chitosan‐CMC capsule

Encapsulation of mammalian cell with chitosan‐CMC capsule

10.1002/bit.260350110

Crossref journal-article

Wiley

Biotechnology and Bioengineering (311)

Abstract

AbstractViable hybridoma cells were encapsulated. The capsules were formed in one step by placing a drop of cell suspension mixed with negatively charged carboxymethylcellulose (CMC) into a positively charged chitosan solution through the interpolymeric ionic interaction between two oppositely charged polymers. These capsules were found to have a mean diameter of about 1. 5 mm and wall thickness of 3 μm. The cells grew in the capsules using supplemented DMEM/F12 (four kinds of growth factor). The maximum cell density in encapsulating cell culture reached 1 × 107 cells/ml, 10 times higher than that obtained in the free cell culture. The maximum monoclonal antibody concentration in the free cell culture was 15μg/mL, but that in the capsule was 45μg/mL The antibody produced by the cell was concentrated about four times higher inside than outside of the capsules.

Bibliography

Yoshioka, T., Hirano, R., Shioya, T., & Kako, M. (1990). Encapsulation of mammalian cell with chitosanâCMC capsule. Biotechnology and Bioengineering, 35(1), 66â72. Portico.

Authors 4

Toshimitsu Yoshioka (first)
Ryogo Hirano (additional)
Toshiaki Shioya (additional)
Masatoshi Kako (additional)

References 41 Referenced 100

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{'key': 'e_1_2_1_42_2', 'first-page': '56', 'volume-title': 'Cell and Tissue Culture', 'author': 'Paul J.', 'year': '1975'} / Cell and Tissue Culture by Paul J. (1975)

Dates

Type	When
Created	20 years, 8 months ago (Dec. 30, 2004, 11:38 a.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 9:59 a.m.)
Indexed	2 months, 1 week ago (June 25, 2025, 12:48 a.m.)
Issued	35 years, 7 months ago (Jan. 5, 1990)
Published	35 years, 7 months ago (Jan. 5, 1990)
Published Online	21 years, 6 months ago (Feb. 18, 2004)
Published Print	35 years, 7 months ago (Jan. 5, 1990)

Funders 0

None

BibTeX

@article{Yoshioka_1990, title={Encapsulation of mammalian cell with chitosan‐CMC capsule}, volume={35}, ISSN={1097-0290}, url={http://dx.doi.org/10.1002/bit.260350110}, DOI={10.1002/bit.260350110}, number={1}, journal={Biotechnology and Bioengineering}, publisher={Wiley}, author={Yoshioka, Toshimitsu and Hirano, Ryogo and Shioya, Toshiaki and Kako, Masatoshi}, year={1990}, month=jan, pages={66–72} }

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