DOI: 10.1002/bit.260281019. Encapsulation of viable cells within polyacrylate membranes

Encapsulation of viable cells within polyacrylate membranes

10.1002/bit.260281019

Crossref journal-article

Wiley

Biotechnology and Bioengineering (311)

Abstract

AbstractA new, semipermeable hydrogel membrane for encapsulating viable cells has been developed. The encapsulation was performed by consecutively introducing droplets of a suspension of hybridoma cells in a solution of a polyanionic acrylic copolymer into aqueous solutions of three polycationic polymers. As a result of interpolymeric ionic interactions and some chemical reactions, a polyelectrolyte complex membrane was formed at the interface between each droplet and the polycationic polymeric solutions. The hybridoma cells, used as a model system, were derived by fusing spleen cells from immunized BALB/c mice with the NS‐1 murine plasmacytoma cell line. The cells divided and gradually filled the microcapsules over a period of 8 days. Prior to encapsulation of the hybridoma cells, the polyanions were tested for toxicity and inhibition of cell growth. A direct relationship was observed between hybridoma cell viability in the acrylic polyanion/RPMI‐1640/10% (w/v) fetal calf serum (FCS) solutions and the kinematic viscosities of the solutions of the polyanions. Antihuman IgM was produced by the encapsulated hybridoma cells and immunoassay showed that the antibody concentration was 3 μg/ml of the total culture medium.

Bibliography

Gharapetian, H., Davies, N. A., & Sun, A. M. (1986). Encapsulation of viable cells within polyacrylate membranes. Biotechnology and Bioengineering, 28(10), 1595â1600. Portico.

Authors 3

H. Gharapetian (first)
N. A. Davies (additional)
A. M. Sun (additional)

References 12 Referenced 33

10.1126/science.6776628
10.1002/jps.2600700402
10.1007/BF02783739
10.1016/0167-4889(84)90107-1
10.1002/bit.260270207
{'key': 'e_1_2_1_7_2', 'first-page': '276', 'volume': '18', 'author': 'Jarvis A. P.', 'year': '1982', 'journal-title': 'In Vitro'} / In Vitro by Jarvis A. P. (1982)
{'key': 'e_1_2_1_8_2', 'first-page': '17', 'volume': '1', 'author': 'Jarvis A. P.', 'year': '1983', 'journal-title': 'Biotech'} / Biotech by Jarvis A. P. (1983)
10.1016/0304-4165(82)90290-2
{'key': 'e_1_2_1_10_2', 'first-page': '75', 'volume': '24', 'author': 'Lamberti F. V.', 'year': '1983', 'journal-title': 'Polym. Prepr., Am. chem. Soc., Div. Polym. Chem.'} / Polym. Prepr., Am. chem. Soc., Div. Polym. Chem. by Lamberti F. V. (1983)
10.1007/BF02783740
10.1021/j100699a005
10.1021/ma60073a011

Dates

Type	When
Created	20 years, 8 months ago (Dec. 29, 2004, 10:57 p.m.)
Deposited	1 year, 10 months ago (Oct. 21, 2023, 3:17 p.m.)
Indexed	1 year, 10 months ago (Oct. 26, 2023, 11:53 a.m.)
Issued	38 years, 11 months ago (Oct. 1, 1986)
Published	38 years, 11 months ago (Oct. 1, 1986)
Published Online	21 years, 6 months ago (Feb. 18, 2004)
Published Print	38 years, 11 months ago (Oct. 1, 1986)

Funders 0

None

BibTeX

@article{Gharapetian_1986, title={Encapsulation of viable cells within polyacrylate membranes}, volume={28}, ISSN={1097-0290}, url={http://dx.doi.org/10.1002/bit.260281019}, DOI={10.1002/bit.260281019}, number={10}, journal={Biotechnology and Bioengineering}, publisher={Wiley}, author={Gharapetian, H. and Davies, N. A. and Sun, A. M.}, year={1986}, month=oct, pages={1595–1600} }

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