DOI: 10.1002/bit.260280817. Characteristics of lipase‐catalyzed hydrolysis of olive oil in AOT

Characteristics of lipase‐catalyzed hydrolysis of olive oil in AOT–isooctane reversed micelles

10.1002/bit.260280817

Crossref journal-article

Wiley

Biotechnology and Bioengineering (311)

Abstract

AbstractCandida rugosa lipase solubilized in organic solvents in the presence of both surfactant and water could catalyze the hydrolysis of triglycerides, and kinetic analysis of the lipase‐catalyzed reaction was found to be possible in this system. Among eight organic solvents tested, isooctane was most effective for the hydrolysis of olive oil in reversed micelles. Temperature effect, pH profile, Km,app and Vmax,app were determined. Among various chemical compounds, Cu2+, Hg2+, and Fe3+ inhibited lipase severely. But the enzyme activity was restorable partially by adding histidine or glycine to the system containing these metal ions. The enzyme activity was dependent on R (molar ratio of water to surfactant) and maximum activity was obtained at R = 10.5. Upon addition of glycerol to the reversed micelles, lipase activity was affected in a different fashion depending on the R values. Stability of the lipase in reversed micelles was also dependent on R, and it was most stable at R = 5.5.

Bibliography

Han, D., & Rhee, J. S. (1986). Characteristics of lipaseâcatalyzed hydrolysis of olive oil in AOTâisooctane reversed micelles. Biotechnology and Bioengineering, 28(8), 1250â1255. Portico.

Authors 2

Daeseok Han (first)
Joon Shick Rhee (additional)

References 26 Referenced 208

10.1016/0006-291X(79)90965-3
10.1021/ja00516a039
10.1016/S0021-9258(19)70054-4
10.1016/0005-2744(81)90151-0
10.1021/ja00404a044
10.1073/pnas.76.2.681
10.1016/0014-5793(83)80451-7
10.1002/9780470122686.ch4
10.1007/BF02697447
{'key': 'e_1_2_1_11_2', 'first-page': '887', 'volume-title': 'Solution Behaviour of Surfactants', 'author': 'Luisi P. L.', 'year': '1980'} / Solution Behaviour of Surfactants by Luisi P. L. (1980)
10.1007/BF01498277
10.1007/BF02636814
10.1007/BF02534737
10.1016/0141-0229(79)90044-9
{'key': 'e_1_2_1_16_2', 'first-page': '13', 'volume-title': 'The Enzyme of Lipid Metabolism', 'author': 'Wills E. D.', 'year': '1961'} / The Enzyme of Lipid Metabolism by Wills E. D. (1961)
10.3168/jds.S0022-0302(66)87874-8
{'key': 'e_1_2_1_18_2', 'first-page': '691', 'volume-title': 'Advances in Biotechnology', 'author': 'Celebi S. S.', 'year': '1981'} / Advances in Biotechnology by Celebi S. S. (1981)
10.1111/j.1748-1716.1945.tb00291.x
10.1016/0006-3002(60)91389-5
10.1021/ja00404a044
10.1016/S0040-4039(01)96107-6
10.1021/ja00456a034
10.1016/0009-2614(75)80134-5
10.1021/j100467a011
10.1002/hlca.19740570708
10.1016/0021-9797(75)90303-3

Dates

Type	When
Created	20 years, 3 months ago (May 27, 2005, 1:23 p.m.)
Deposited	1 year, 10 months ago (Oct. 20, 2023, 12:56 p.m.)
Indexed	2 months, 2 weeks ago (June 10, 2025, 3:04 p.m.)
Issued	39 years ago (Aug. 1, 1986)
Published	39 years ago (Aug. 1, 1986)
Published Online	21 years, 6 months ago (Feb. 18, 2004)
Published Print	39 years ago (Aug. 1, 1986)

Funders 0

None

BibTeX

@article{Han_1986, title={Characteristics of lipase‐catalyzed hydrolysis of olive oil in AOT–isooctane reversed micelles}, volume={28}, ISSN={1097-0290}, url={http://dx.doi.org/10.1002/bit.260280817}, DOI={10.1002/bit.260280817}, number={8}, journal={Biotechnology and Bioengineering}, publisher={Wiley}, author={Han, Daeseok and Rhee, Joon Shick}, year={1986}, month=aug, pages={1250–1255} }

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