DOI: 10.1002/bit.260220105. Preparation of immobilized phosphodiesterase from calf spleen and its use in oligonucleotide analysis

Preparation of immobilized phosphodiesterase from calf spleen and its use in oligonucleotide analysis

10.1002/bit.260220105

Crossref journal-article

Wiley

Biotechnology and Bioengineering (311)

Abstract

AbstractThe phosphodiesterase from calf spleen (EC 3.1.4.18) was immobilized on several supports. Some properties of the most suitable enzyme support system—calf spleen phosphodiesterase bound to agarose‐Concanavalin A—were investigated, e.g., pH dependence, influence of ionic strength of the buffer medium, and Zn2+‐ion inhibition. The immobilized spleen phosphodiesterase showed about 60% of the activity of the free enzyme: the activity toward several oligonucleotide test substrates was unchanged for two months.

Bibliography

Seliger, H., Teufel, E. H., & Philipp, M. (1980). Preparation of immobilized phosphodiesterase from calf spleen and its use in oligonucleotide analysis. Biotechnology and Bioengineering, 22(1), 55â64. Portico.

Authors 3

H. Seliger (first)
E. H. Teufel (additional)
M. Philipp (additional)

References 18 Referenced 4

10.1016/S0021-9258(18)64257-7 / J. Biol. Chem. by Razzell W. E. (1961)
{'key': 'e_1_2_1_3_2', 'first-page': '245', 'volume-title': 'Methods in Enzymology', 'author': 'Razzell W. E.', 'year': '1963'} / Methods in Enzymology by Razzell W. E. (1963)
{'key': 'e_1_2_1_4_2', 'first-page': '329', 'volume-title': 'The Enzymes', 'author': 'Bernardi A.', 'year': '1971'} / The Enzymes by Bernardi A. (1971)
{'key': 'e_1_2_1_5_2', 'first-page': '79', 'volume-title': 'The Enzymes', 'author': 'Khorana H. G.', 'year': '1961'} / The Enzymes by Khorana H. G. (1961)
M.PhilippandH.Seliger Abstracts of the FEBS Symposium on Immobilized Enzymes Szeged Hungary 1974 p.41.
{'key': 'e_1_2_1_7_2', 'first-page': '266', 'volume-title': 'Abstracts of the XXVIth International Congress of Pure and Applied Chemistry', 'author': 'Philipp M.', 'year': '1977'} / Abstracts of the XXVIth International Congress of Pure and Applied Chemistry by Philipp M. (1977)
10.1016/0003-2697(74)90139-0
10.1021/bi00838a026
10.1016/0006-291X(76)91101-3
10.1016/0003-2697(74)90097-9
10.1016/0006-291X(74)90954-1
10.1016/S0021-9258(18)69374-3 / J. Biol. Chem. by Hilmoe A. J. (1960)
10.1016/0005-2787(68)90180-9
10.1021/bi00900a022
10.1016/0003-9861(68)90322-6
10.1093/nar/4.10.3511
10.1042/bj0550170
{'key': 'e_1_2_1_19_2', 'volume-title': 'Enzym‐Kinetik', 'author': 'Ahlers J.', 'year': '1974'} / Enzym‐Kinetik by Ahlers J. (1974)

Dates

Type	When
Created	20 years, 8 months ago (Dec. 29, 2004, 10:32 p.m.)
Deposited	1 year, 9 months ago (Nov. 12, 2023, 3:24 a.m.)
Indexed	1 year, 9 months ago (Nov. 12, 2023, 3:40 a.m.)
Issued	45 years, 8 months ago (Jan. 1, 1980)
Published	45 years, 8 months ago (Jan. 1, 1980)
Published Online	21 years, 6 months ago (Feb. 18, 2004)
Published Print	45 years, 8 months ago (Jan. 1, 1980)

Funders 0

None

BibTeX

@article{Seliger_1980, title={Preparation of immobilized phosphodiesterase from calf spleen and its use in oligonucleotide analysis}, volume={22}, ISSN={1097-0290}, url={http://dx.doi.org/10.1002/bit.260220105}, DOI={10.1002/bit.260220105}, number={1}, journal={Biotechnology and Bioengineering}, publisher={Wiley}, author={Seliger, H. and Teufel, E. H. and Philipp, M.}, year={1980}, month=jan, pages={55–64} }

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