DOI: 10.1002/bit.260190309. The immobilization of microbial cells, subcellular organelles, and enzymes in calcium alginate gels

The immobilization of microbial cells, subcellular organelles, and enzymes in calcium alginate gels

10.1002/bit.260190309

Crossref journal-article

Wiley

Biotechnology and Bioengineering (311)

Abstract

AbstractSaccharomyces cerevisiae cells, Kluyveromyces marxianus cells, inulase, glucose oxidase, chloroplasts, and mitochondria were immobilized in calcium alginate gels.Ethanol production from glucose solutions by an immobilized preparation of S. cerevisiae was demonstrated over a total of twenty‐three days, and the half‐life of such a preparation was shown to be about ten days.Immobilized K. marxianus, inulase, and glucose oxidase preparations were used to demonstrate the porosity and retraining properties of calcium alginate gels.Calcium alginate–immobilized chloroplasts were shown to perform the Hill reaction.Some experiments with immobilized mitochondria are reported.

Bibliography

Kierstan, M., & Bucke, C. (1977). The immobilization of microbial cells, subcellular organelles, and enzymes in calcium alginate gels. Biotechnology and Bioengineering, 19(3), 387â397. Portico.

Authors 2

M. Kierstan (first)
C. Bucke (additional)

References 19 Referenced 526

10.1042/bj1070001
10.1042/bj1080845
10.1016/0006-291X(71)90606-1
10.3891/acta.chem.scand.24-2093
10.1016/0005-2744(71)90054-4
10.1002/bit.260150312
10.1246/bcsj.44.3201
{'key': 'e_1_2_1_9_2', 'first-page': '17', 'volume': '9', 'year': '1976', 'journal-title': 'Anon, Chemical and Engineering News.'} / Anon, Chemical and Engineering News. (1976)
10.1007/BF01382687
U.S. Patent 3 733 205 May 15th 1973.
10.1016/S0031-9422(00)82910-6
10.1038/2161208a0
{'key': 'e_1_2_1_14_2', 'first-page': '123', 'volume-title': 'Methods in Enzymatic Analysis', 'author': 'Bergmeyer H. V.', 'year': '1963'} / Methods in Enzymatic Analysis by Bergmeyer H. V. (1963)
R.Bonnidisen in Ref. 13 p.285.
10.1104/pp.40.6.1157
W.LamprechtandI.Trautschold in Ref. 13 p.543.
10.1016/S0021-9258(18)71980-7
{'key': 'e_1_2_1_19_2', 'first-page': 'xLiv', 'volume': '56', 'author': 'Asatoor A.', 'year': '1954', 'journal-title': 'Biochem. J.'} / Biochem. J. by Asatoor A. (1954)
D. F.Pindar private communication.

Dates

Type	When
Created	20 years, 7 months ago (Dec. 29, 2004, 2:16 p.m.)
Deposited	1 year, 9 months ago (Nov. 12, 2023, 5:39 a.m.)
Indexed	2 months, 3 weeks ago (June 4, 2025, 5:12 p.m.)
Issued	48 years, 5 months ago (March 1, 1977)
Published	48 years, 5 months ago (March 1, 1977)
Published Online	21 years, 6 months ago (Feb. 18, 2004)
Published Print	48 years, 5 months ago (March 1, 1977)

Funders 0

None

BibTeX

@article{Kierstan_1977, title={The immobilization of microbial cells, subcellular organelles, and enzymes in calcium alginate gels}, volume={19}, ISSN={1097-0290}, url={http://dx.doi.org/10.1002/bit.260190309}, DOI={10.1002/bit.260190309}, number={3}, journal={Biotechnology and Bioengineering}, publisher={Wiley}, author={Kierstan, M. and Bucke, C.}, year={1977}, month=mar, pages={387–397} }

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