DOI: 10.1002/bit.22102. Photo‐immobilization of biological components on gold‐coated chips for measurements using surface plasmon resonance (SPR) and a quartz crystal microbalance (QCM)

Photo‐immobilization of biological components on gold‐coated chips for measurements using surface plasmon resonance (SPR) and a quartz crystal microbalance (QCM)

10.1002/bit.22102

Crossref journal-article

Wiley

Biotechnology and Bioengineering (311)

Abstract

AbstractThe photo‐immobilization technique is useful for immobilization of various biomolecules on assorted material surfaces, independent of the organic functional groups that may be present. Here, we report a convenient new photo‐immobilization technique that was developed by combining a nonbiofouling polymer containing polyethylene glycol and a photoreactive crosslinker for surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) measurements. By this method, nonspecific interactions were reduced and various types of molecules, bovine serum albumin, heparin, dsDNA, phosphatidylserine, Tobacco Mosaic Virus, and norfloxacine, were immobilized on an alkane thiol‐modified gold surface by a single method. The interactions of photo‐immobilized biomolecules and their corresponding antibodies were investigated by SPR and QCM. In addition, SPR imaging was possible using the present method. Biotechnol. Bioeng. 2009; 102: 700–707. © 2008 Wiley Periodicals, Inc.

Bibliography

Tsuzuki, S., Wada, A., & Ito, Y. (2008). Photoâimmobilization of biological components on goldâcoated chips for measurements using surface plasmon resonance (SPR) and a quartz crystal microbalance (QCM). Biotechnology and Bioengineering, 102(3), 700â707. Portico.

Authors 3

Saki Tsuzuki (first)
Akira Wada (additional)
Yoshihiro Ito (additional)

References 30 Referenced 15

10.1021/bc700287v
10.1002/bit.20882
10.1016/S0968-0896(01)00172-9
10.1021/bc00024a016
{'key': 'e_1_2_1_6_1', 'first-page': '785', 'volume-title': 'Bioconjugate techniques', 'author': 'Hermanson GH', 'year': '1996'} / Bioconjugate techniques by Hermanson GH (1996)
10.1021/la063406j
10.1021/bp060143a
10.1016/S0142-9612(03)00134-0
10.1295/koron.61.501
10.1016/j.biomaterials.2004.02.019
10.1002/bit.21725
10.1002/bit.20253
{'key': 'e_1_2_1_14_1', 'first-page': '291', 'volume-title': 'Advances in biosensors', 'author': 'Jönsson U', 'year': '1992'} / Advances in biosensors by Jönsson U (1992)
10.1002/anie.200352164
10.1021/ac051777j
10.1002/asia.200600208
10.1021/bm0101670
10.1006/abio.2001.5168
10.1021/la063697w
10.1021/la0102307
10.2332/allergolint.54.627
10.1002/9780470771266
10.1002/cbic.200600447
10.1021/la702043t
10.1021/bm061197b
10.1021/ac025526b
10.1021/bi034113h
10.1021/ma00104a040
10.1021/ja056422w
10.1021/bc00026a007

Dates

Type	When
Created	16 years, 11 months ago (Sept. 24, 2008, 3:54 p.m.)
Deposited	1 year, 10 months ago (Oct. 13, 2023, 8:19 p.m.)
Indexed	1 month, 2 weeks ago (July 20, 2025, 6:54 p.m.)
Issued	16 years, 9 months ago (Nov. 6, 2008)
Published	16 years, 9 months ago (Nov. 6, 2008)
Published Online	16 years, 9 months ago (Nov. 6, 2008)
Published Print	16 years, 6 months ago (Feb. 15, 2009)

Funders 0

None

BibTeX

@article{Tsuzuki_2008, title={Photo‐immobilization of biological components on gold‐coated chips for measurements using surface plasmon resonance (SPR) and a quartz crystal microbalance (QCM)}, volume={102}, ISSN={1097-0290}, url={http://dx.doi.org/10.1002/bit.22102}, DOI={10.1002/bit.22102}, number={3}, journal={Biotechnology and Bioengineering}, publisher={Wiley}, author={Tsuzuki, Saki and Wada, Akira and Ito, Yoshihiro}, year={2008}, month=nov, pages={700–707} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        20
      ]
    ],
    "date-time": "2025-07-20T22:54:37Z",
    "timestamp": 1753052077729
  },
  "reference-count": 30,
  "publisher": "Wiley",
  "issue": "3",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2008,
            9,
            24
          ]
        ],
        "date-time": "2008-09-24T00:00:00Z",
        "timestamp": 1222214400000
      },
      "content-version": "vor",
      "delay-in-days": 0,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2009,
        2,
        15
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>The photo\u2010immobilization technique is useful for immobilization of various biomolecules on assorted material surfaces, independent of the organic functional groups that may be present. Here, we report a convenient new photo\u2010immobilization technique that was developed by combining a nonbiofouling polymer containing polyethylene glycol and a photoreactive crosslinker for surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) measurements. By this method, nonspecific interactions were reduced and various types of molecules, bovine serum albumin, heparin, dsDNA, phosphatidylserine, Tobacco Mosaic Virus, and norfloxacine, were immobilized on an alkane thiol\u2010modified gold surface by a single method. The interactions of photo\u2010immobilized biomolecules and their corresponding antibodies were investigated by SPR and QCM. In addition, SPR imaging was possible using the present method. Biotechnol. Bioeng. 2009; 102: 700\u2013707. \u00a9 2008 Wiley Periodicals, Inc.</jats:p>",
  "DOI": "10.1002/bit.22102",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2008,
        9,
        24
      ]
    ],
    "date-time": "2008-09-24T19:54:20Z",
    "timestamp": 1222286060000
  },
  "page": "700-707",
  "source": "Crossref",
  "is-referenced-by-count": 15,
  "title": "Photo\u2010immobilization of biological components on gold\u2010coated chips for measurements using surface plasmon resonance (SPR) and a quartz crystal microbalance (QCM)",
  "prefix": "10.1002",
  "volume": "102",
  "author": [
    {
      "given": "Saki",
      "family": "Tsuzuki",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Akira",
      "family": "Wada",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Yoshihiro",
      "family": "Ito",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2008,
        11,
        6
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bc700287v"
    },
    {
      "key": "e_1_2_1_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/bit.20882"
    },
    {
      "key": "e_1_2_1_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0968-0896(01)00172-9"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bc00024a016"
    },
    {
      "key": "e_1_2_1_6_1",
      "first-page": "785",
      "volume-title": "Bioconjugate techniques",
      "author": "Hermanson GH",
      "year": "1996"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/la063406j"
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bp060143a"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0142-9612(03)00134-0"
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1295/koron.61.501"
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.biomaterials.2004.02.019"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/bit.21725"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/bit.20253"
    },
    {
      "key": "e_1_2_1_14_1",
      "first-page": "291",
      "volume-title": "Advances in biosensors",
      "author": "J\u00f6nsson U",
      "year": "1992"
    },
    {
      "key": "e_1_2_1_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/anie.200352164"
    },
    {
      "key": "e_1_2_1_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/ac051777j"
    },
    {
      "key": "e_1_2_1_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/asia.200600208"
    },
    {
      "key": "e_1_2_1_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bm0101670"
    },
    {
      "key": "e_1_2_1_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/abio.2001.5168"
    },
    {
      "key": "e_1_2_1_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/la063697w"
    },
    {
      "key": "e_1_2_1_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/la0102307"
    },
    {
      "key": "e_1_2_1_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.2332/allergolint.54.627"
    },
    {
      "key": "e_1_2_1_23_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/9780470771266"
    },
    {
      "key": "e_1_2_1_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/cbic.200600447"
    },
    {
      "key": "e_1_2_1_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/la702043t"
    },
    {
      "key": "e_1_2_1_26_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bm061197b"
    },
    {
      "key": "e_1_2_1_27_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/ac025526b"
    },
    {
      "key": "e_1_2_1_28_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi034113h"
    },
    {
      "key": "e_1_2_1_29_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/ma00104a040"
    },
    {
      "key": "e_1_2_1_30_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/ja056422w"
    },
    {
      "key": "e_1_2_1_31_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bc00026a007"
    }
  ],
  "container-title": "Biotechnology and Bioengineering",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbit.22102",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.22102",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        14
      ]
    ],
    "date-time": "2023-10-14T00:19:07Z",
    "timestamp": 1697242747000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1002/bit.22102"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2008,
        11,
        6
      ]
    ]
  },
  "references-count": 30,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          2009,
          2,
          15
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1002/bit.22102"
  ],
  "URL": "http://dx.doi.org/10.1002/bit.22102",
  "relation": {},
  "ISSN": [
    "0006-3592",
    "1097-0290"
  ],
  "subject": [],
  "container-title-short": "Biotech &amp; Bioengineering",
  "published": {
    "date-parts": [
      [
        2008,
        11,
        6
      ]
    ]
  }
}