DOI: 10.1002/bit.20882. Antibody‐based surface plasmon resonance detection of intact viral pathogen

Antibody‐based surface plasmon resonance detection of intact viral pathogen

10.1002/bit.20882

Crossref journal-article

Wiley

Biotechnology and Bioengineering (311)

Abstract

AbstractSurface plasmon resonance (SPR) technique was used to directly detect an intact form of insect pathogen: the baculovirus, Autographa californica multiple nuclear polyhedrosis virus (AcMNPV). An SPR sensor chip with three bio‐functional layers was used to detect the intact AcMNPV: amine‐reactive crosslinker with a disulfide bond that chemisorbs to gold film, Protein A, and a mouse IgG monoclonal antibody raised against a surface protein of the target viral pathogen. A two‐channel (reference & test) micro‐fluidic SPR system is used for reliable measurement. Bio‐specific response to the AcMNPV is compared with the response for tobacco mosaic virus (TMV) as control. Successive exposure of the sensor chip to both viruses verifies a specific response to AcMNPV. This serves as a prerequisite to the development of a new type of viral pathogen detection sensors. © 2006 Wiley Periodicals, Inc.

Bibliography

Baac, H., HajÃ³s, J. P., Lee, J., Kim, D., Kim, S. J., & Shuler, M. L. (2006). Antibodyâbased surface plasmon resonance detection of intact viral pathogen. Biotechnology and Bioengineering, 94(4), 815â819. Portico.

Authors 6

Hyoungwon Baac (first)
József P. Hajós (additional)
Jennifer Lee (additional)
Donghyun Kim (additional)
Sung June Kim (additional)
Michael L. Shuler (additional)

References 14 Referenced 50

10.1016/j.jviromet.2004.06.019
10.1023/A:1008872002336
10.1006/abio.2001.5535
10.1007/s00216-003-2101-0
10.1016/S0168-1605(02)00010-7
10.1016/S0925-4005(00)00717-6
10.1016/0925-4005(93)85239-7
10.1006/abio.2000.4564
10.1016/j.snb.2003.11.014
{'key': 'e_1_2_1_11_1', 'volume-title': 'Baculovirus expression systems and biopesticides', 'author': 'Shuler ML', 'year': '1995'} / Baculovirus expression systems and biopesticides by Shuler ML (1995)
10.1006/viro.2000.0252
10.1063/1.1150128
10.1016/S0166-0934(00)00160-9
10.1063/1.1523649

Dates

Type	When
Created	19 years, 6 months ago (Feb. 9, 2006, 3:28 p.m.)
Deposited	1 year, 10 months ago (Oct. 13, 2023, 11:16 a.m.)
Indexed	1 month, 1 week ago (July 26, 2025, 5:32 a.m.)
Issued	19 years, 6 months ago (Feb. 7, 2006)
Published	19 years, 6 months ago (Feb. 7, 2006)
Published Online	19 years, 6 months ago (Feb. 7, 2006)
Published Print	19 years, 2 months ago (July 5, 2006)

Funders 0

None

BibTeX

@article{Baac_2006, title={Antibody‐based surface plasmon resonance detection of intact viral pathogen}, volume={94}, ISSN={1097-0290}, url={http://dx.doi.org/10.1002/bit.20882}, DOI={10.1002/bit.20882}, number={4}, journal={Biotechnology and Bioengineering}, publisher={Wiley}, author={Baac, Hyoungwon and Hajós, József P. and Lee, Jennifer and Kim, Donghyun and Kim, Sung June and Shuler, Michael L.}, year={2006}, month=feb, pages={815–819} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        26
      ]
    ],
    "date-time": "2025-07-26T09:32:52Z",
    "timestamp": 1753522372128
  },
  "reference-count": 14,
  "publisher": "Wiley",
  "issue": "4",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2006,
            2,
            7
          ]
        ],
        "date-time": "2006-02-07T00:00:00Z",
        "timestamp": 1139270400000
      },
      "content-version": "vor",
      "delay-in-days": 0,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2006,
        7,
        5
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>Surface plasmon resonance (SPR) technique was used to directly detect an intact form of insect pathogen: the baculovirus, <jats:italic>Autographa californica</jats:italic> multiple nuclear polyhedrosis virus (AcMNPV). An SPR sensor chip with three bio\u2010functional layers was used to detect the intact AcMNPV: amine\u2010reactive crosslinker with a disulfide bond that chemisorbs to gold film, Protein A, and a mouse IgG monoclonal antibody raised against a surface protein of the target viral pathogen. A two\u2010channel (reference &amp; test) micro\u2010fluidic SPR system is used for reliable measurement. Bio\u2010specific response to the AcMNPV is compared with the response for tobacco mosaic virus (TMV) as control. Successive exposure of the sensor chip to both viruses verifies a specific response to AcMNPV. This serves as a prerequisite to the development of a new type of viral pathogen detection sensors. \u00a9 2006 Wiley Periodicals, Inc.</jats:p>",
  "DOI": "10.1002/bit.20882",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        2,
        9
      ]
    ],
    "date-time": "2006-02-09T20:28:55Z",
    "timestamp": 1139516935000
  },
  "page": "815-819",
  "source": "Crossref",
  "is-referenced-by-count": 50,
  "title": "Antibody\u2010based surface plasmon resonance detection of intact viral pathogen",
  "prefix": "10.1002",
  "volume": "94",
  "author": [
    {
      "given": "Hyoungwon",
      "family": "Baac",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "J\u00f3zsef P.",
      "family": "Haj\u00f3s",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Jennifer",
      "family": "Lee",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Donghyun",
      "family": "Kim",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Sung June",
      "family": "Kim",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Michael L.",
      "family": "Shuler",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2006,
        2,
        7
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.jviromet.2004.06.019"
    },
    {
      "key": "e_1_2_1_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1023/A:1008872002336"
    },
    {
      "key": "e_1_2_1_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/abio.2001.5535"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/s00216-003-2101-0"
    },
    {
      "key": "e_1_2_1_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0168-1605(02)00010-7"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0925-4005(00)00717-6"
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0925-4005(93)85239-7"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/abio.2000.4564"
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.snb.2003.11.014"
    },
    {
      "key": "e_1_2_1_11_1",
      "volume-title": "Baculovirus expression systems and biopesticides",
      "author": "Shuler ML",
      "year": "1995"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/viro.2000.0252"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1063/1.1150128"
    },
    {
      "key": "e_1_2_1_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0166-0934(00)00160-9"
    },
    {
      "key": "e_1_2_1_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1063/1.1523649"
    }
  ],
  "container-title": "Biotechnology and Bioengineering",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fbit.20882",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/bit.20882",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        13
      ]
    ],
    "date-time": "2023-10-13T15:16:46Z",
    "timestamp": 1697210206000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1002/bit.20882"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2006,
        2,
        7
      ]
    ]
  },
  "references-count": 14,
  "journal-issue": {
    "issue": "4",
    "published-print": {
      "date-parts": [
        [
          2006,
          7,
          5
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1002/bit.20882"
  ],
  "URL": "http://dx.doi.org/10.1002/bit.20882",
  "relation": {},
  "ISSN": [
    "0006-3592",
    "1097-0290"
  ],
  "subject": [],
  "container-title-short": "Biotech &amp; Bioengineering",
  "published": {
    "date-parts": [
      [
        2006,
        2,
        7
      ]
    ]
  }
}