DOI: 10.1002/bit.20313. Electrochemical probe for on‐chip type flow immunoassay: Immunoglobulin G labeled with ferrocenecarboaldehyde

Electrochemical probe for on‐chip type flow immunoassay: Immunoglobulin G labeled with ferrocenecarboaldehyde

10.1002/bit.20313

Crossref journal-article

Wiley

Biotechnology and Bioengineering (311)

Abstract

AbstractLabeling of ferrocenecarboaldehyde (Fc‐CHO) to immunoglobulin G (IgG) via formation of Schiff‐base and its reduction was investigated for construction of an electrochemical probe for miniaturized amperometric flow immunoassay. Approximately eight molecules of Fc‐CHO were labeled to IgG and the reversible redox property of ferrocene was observed. Labeling efficiency improved by over three times as compared to the conventional method using ferrocenemonocarboxylic acid (Fc‐COOH). Also, binding affinity of IgG labeled with Fc‐CHO to its antigen, IgE, was investigated by enzyme‐linked immunosorbent assay (ELISA) and surface plasmon resonance assay. IgG labeled with Fc‐CHO that retained eight ferrocene moiety showed sufficient binding affinity to its antigen and the current response obtained in the flow electrochemical detection system increased by 14‐fold as compared with IgG labeled with Fc‐COOH when applying the potential of 390 mV vs. Ag/AgCl. The minimum detectable concentration of IgG labeled with Fc‐CHO was 0.06 μM. IgG labeled with Fc‐CHO demonstrate biochemical and electrochemical properties that are useful for electrochemical immunosensors. © 2005 Wiley Periodicals, Inc.

Bibliography

Okochi, M., Ohta, H., Tanaka, T., & Matsunaga, T. (2005). Electrochemical probe for onâchip type flow immunoassay: Immunoglobulin G labeled with ferrocenecarboaldehyde. Biotechnology and Bioengineering, 90(1), 14â19. Portico.

Authors 4

Mina Okochi (first)
Hiroko Ohta (additional)
Tsuyoshi Tanaka (additional)
Tadashi Matsunaga (additional)

References 26 Referenced 30

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{'issue': '2', 'key': 'e_1_2_1_23_1', 'first-page': '291', 'article-title': 'An anthracene derivative carrying ferrocenyl moieties at its 9 and 10 positions as a new electrochemically active threading intercalator', 'author': 'Takenaka S', 'year': '2003', 'journal-title': 'Nucleic Acids Res'} / Nucleic Acids Res / An anthracene derivative carrying ferrocenyl moieties at its 9 and 10 positions as a new electrochemically active threading intercalator by Takenaka S (2003)
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Dates

Type	When
Created	20 years, 6 months ago (Feb. 25, 2005, 7:18 p.m.)
Deposited	1 year, 10 months ago (Oct. 13, 2023, 7:07 p.m.)
Indexed	5 months, 2 weeks ago (March 19, 2025, 7 a.m.)
Issued	20 years, 6 months ago (Feb. 25, 2005)
Published	20 years, 6 months ago (Feb. 25, 2005)
Published Online	20 years, 6 months ago (Feb. 25, 2005)
Published Print	20 years, 4 months ago (April 5, 2005)

Funders 0

None

BibTeX

@article{Okochi_2005, title={Electrochemical probe for on‐chip type flow immunoassay: Immunoglobulin G labeled with ferrocenecarboaldehyde}, volume={90}, ISSN={1097-0290}, url={http://dx.doi.org/10.1002/bit.20313}, DOI={10.1002/bit.20313}, number={1}, journal={Biotechnology and Bioengineering}, publisher={Wiley}, author={Okochi, Mina and Ohta, Hiroko and Tanaka, Tsuyoshi and Matsunaga, Tadashi}, year={2005}, month=feb, pages={14–19} }

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