DOI: 10.1002/bit.10463. Substrate and product inhibition of hydrogen production by the extreme thermophile, <i>Caldicellulosiruptor saccharolyticus</i>

Substrate and product inhibition of hydrogen production by the extreme thermophile, Caldicellulosiruptor saccharolyticus

10.1002/bit.10463

Crossref journal-article

Wiley

Biotechnology and Bioengineering (311)

Abstract

AbstractSubstrate and product inhibition of hydrogen production during sucrose fermentation by the extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus was studied. The inhibition kinetics were analyzed with a noncompetitive, nonlinear inhibition model. Hydrogen was the most severe inhibitor when allowed to accumulate in the culture. Concentrations of 5–10 mM H2 in the gas phase (≡ partial hydrogen pressure (pH2) of (1–2) · 104 Pa) initiated a metabolic shift to lactate formation. The extent of inhibition by hydrogen was dependent on the density of the culture. The highest tolerance for hydrogen was found at low volumetric hydrogen production rates, as occurred in cultures with low cell densities. Under those conditions the critical hydrogen concentration in the gas phase was 27.7 mM H2 (≡ pH2 of 5.6 · 104 Pa); above this value hydrogen production ceased completely. With an efficient removal of hydrogen sucrose fermentation was mainly inhibited by sodium acetate. The critical concentrations of sucrose and acetate, at which growth and hydrogen production was completely inhibited (at neutral pH and 70°C), were 292 and 365 mM, respectively. Inorganic salts, such as sodium chloride, mimicked the effect of sodium acetate, implying that ionic strength was responsible for inhibition. Undissociated acetate did not contribute to inhibition of cultures at neutral or slightly acidic pH. Exposure of exponentially growing cultures to concentrations of sodium acetate or sodium chloride higher than ca. 175 mM caused cell lysis, probably due to activation of autolysins. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 81: 255–262, 2003.

Bibliography

van Niel, E. W. J., Claassen, P. A. M., & Stams, A. J. M. (2002). Substrate and product inhibition of hydrogen production by the extreme thermophile, Caldicellulosiruptor saccharolyticus. Biotechnology and Bioengineering, 81(3), 255â262. Portico.

Authors 3

Ed W. J. van Niel (first)
Pieternel A. M. Claassen (additional)
Alfons J. M. Stams (additional)

References 29 Referenced 264

10.1111/j.1574-6968.1990.tb04096.x
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{'key': 'e_1_2_1_12_1', 'first-page': '213', 'article-title': 'Polyamines of thermophilic Gram‐positive anaerobes belonging to the genera Caldicellulosiruptor, Caloramator, Clostridium, Coprothermobacter, Moorella, Thermoanaerobacter and Thermoanaerobacterium', 'volume': '85', 'author': 'Hamana K', 'year': '1996', 'journal-title': 'Microbios'} / Microbios / Polyamines of thermophilic Gram‐positive anaerobes belonging to the genera Caldicellulosiruptor, Caloramator, Clostridium, Coprothermobacter, Moorella, Thermoanaerobacter and Thermoanaerobacterium by Hamana K (1996)
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Dates

Type	When
Created	22 years, 8 months ago (Dec. 10, 2002, 8:46 a.m.)
Deposited	1 year, 10 months ago (Oct. 7, 2023, 9:17 a.m.)
Indexed	16 hours, 2 minutes ago (Aug. 31, 2025, 6:33 a.m.)
Issued	22 years, 8 months ago (Dec. 3, 2002)
Published	22 years, 8 months ago (Dec. 3, 2002)
Published Online	22 years, 8 months ago (Dec. 3, 2002)
Published Print	22 years, 6 months ago (Feb. 5, 2003)

Funders 0

None

BibTeX

@article{van_Niel_2002, title={Substrate and product inhibition of hydrogen production by the extreme thermophile, Caldicellulosiruptor saccharolyticus}, volume={81}, ISSN={1097-0290}, url={http://dx.doi.org/10.1002/bit.10463}, DOI={10.1002/bit.10463}, number={3}, journal={Biotechnology and Bioengineering}, publisher={Wiley}, author={van Niel, Ed W. J. and Claassen, Pieternel A. M. and Stams, Alfons J. M.}, year={2002}, month=dec, pages={255–262} }

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