DOI: 10.1002/bit.10190. A biosensor for the detection of triazine and phenylurea herbicides designed using Photosystem II coupled to a screen‐printed electrode

A biosensor for the detection of triazine and phenylurea herbicides designed using Photosystem II coupled to a screen‐printed electrode

10.1002/bit.10190

Crossref journal-article

Wiley

Biotechnology and Bioengineering (311)

Abstract

AbstractA biosensor for the detection of triazine‐ and phenylurea‐type herbicides was constructed using isolated Photosystem II (PS II) complexes as a biosensing element. PSII isolated from the thermophilic cyanobacterium Synechococcus elongatus was immobilized on the surface of a screen‐printed sensor composed of a graphite working electrode and Ag/AgCl reference electrode deposited on a polymeric substrate. The biosensor was mounted in a flow microcell with illumination. The principle of the detection was based on the fact that herbicides selectively block PSII electron transport activity in a concentration‐dependent manner. Changes of the activity were registered amperometrically as the rate of photoreduction of an artificial electron acceptor. The setup resulted in a reusable herbicide biosensor with a good stability (half‐life of 24 h) and limit of detection of approximately 10−9 M for diuron, atrazine and simazine. © 2002 Wiley Periodicals, Inc. Biotechnol Bioeng 78: 110–116, 2002; DOI 10.1002/bit.10190

Bibliography

KoblÃÅ¾ek, M., MalÃ½, J., MasojÃdek, J., Komenda, J., KuÄera, T., Giardi, M. T., Mattoo, A. K., & Pilloton, R. (2002). A biosensor for the detection of triazine and phenylurea herbicides designed using Photosystem II coupled to a screenâprinted electrode. Biotechnology and Bioengineering, 78(1), 110â116. Portico.

Authors 8

Michal Koblížek (first)
Jan Malý (additional)
Jiří Masojídek (additional)
Josef Komenda (additional)
Tomáš Kučera (additional)
Maria T. Giardi (additional)
Autar K. Mattoo (additional)
Roberto Pilloton (additional)

References 30 Referenced 104

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10.1007/BF01060357
10.1007/BF02182509
10.1002/anie.199116211
GiardiMT EspositoD LeonardiC MattooA MargonelliA AngeliniG.2000. Portable system for selective monitoring of herbicides based on fluorescence and resistant mutants to herbicides. Patent Upica Italy 112.
10.1021/jf00030a034
10.1104/pp.36.6.788
{'key': 'e_1_2_1_10_1', 'first-page': '372', 'volume-title': 'Techniques and instrumentation in analytical chemistry. Flow injection analysis, Vol. 10', 'author': 'Karlberg B', 'year': '1989'} / Techniques and instrumentation in analytical chemistry. Flow injection analysis, Vol. 10 by Karlberg B (1989)
10.1002/(SICI)1097-0290(19981220)60:6<664::AID-BIT3>3.0.CO;2-B
10.1093/jxb/47.2.259
10.1002/bit.260440206
10.1007/s0021663540299 / Fresenius J Anal Chem / Chlorophyll fluorescence biosensor for the detection of herbicides by Merz D (1996)
{'key': 'e_1_2_1_15_1', 'first-page': '12l', 'article-title': 'Research on biochemistry of herbicides: An historical overview', 'volume': '48', 'author': 'Moreland DE', 'year': '1992', 'journal-title': 'Z Naturforsch'} / Z Naturforsch / Research on biochemistry of herbicides: An historical overview by Moreland DE (1992)
10.1016/S0021-9673(96)00408-6
{'key': 'e_1_2_1_17_1', 'first-page': '482', 'volume-title': 'Handbook of sensors and actuators 1: Thick film sensors', 'author': 'Prudenziati M', 'year': '1994'} / Handbook of sensors and actuators 1: Thick film sensors by Prudenziati M (1994)
10.1016/0925-4005(94)01645-X
{'key': 'e_1_2_1_19_1', 'first-page': '360', 'volume-title': 'Techniques and instrumentation in analytical chemistry, Vol. 11. Biosensors', 'author': 'Scheller F', 'year': '1992'} / Techniques and instrumentation in analytical chemistry, Vol. 11. Biosensors by Scheller F (1992)
10.1021/jf00015a033
10.1021/jf00038a033
10.1023/A:1007047703388
10.1002/prot.340210304
10.1023/A:1007051804296
{'key': 'e_1_2_1_25_1', 'first-page': '424', 'article-title': 'Photochemical activities of immobilized chloroplasts isolated from water‐stressed leaves of different ages', 'volume': '24', 'author': 'Synková H', 'year': '1990', 'journal-title': 'Photosynthetica'} / Photosynthetica / Photochemical activities of immobilized chloroplasts isolated from water‐stressed leaves of different ages by Synková H (1990)
{'key': 'e_1_2_1_26_1', 'first-page': '1', 'article-title': 'Immobilization of chloroplasts from young and old leaves of lettuce, pea, spinach and tobacco', 'volume': '25', 'author': 'Synková H', 'year': '1991', 'journal-title': 'Photosynthetica'} / Photosynthetica / Immobilization of chloroplasts from young and old leaves of lettuce, pea, spinach and tobacco by Synková H (1991)
10.1002/bit.260320607
10.1515/znc-1987-0616 / Z Naturforsch / The three‐dimensional structure of the herbicide binding niche on the reaction center polypeptides of photosystem II by Trebst A (1986)
10.1126/science.290.5495.1315
{'key': 'e_1_2_1_30_1', 'first-page': '548‐549', 'article-title': 'The action of some derivatives of phenylurea and of 3‐phenyl‐1,1‐dimethylurea on the Hill reaction', 'volume': '19', 'author': 'Wessels JSC', 'year': '1956', 'journal-title': 'Biochim Biophys Acta'} / Biochim Biophys Acta / The action of some derivatives of phenylurea and of 3‐phenyl‐1,1‐dimethylurea on the Hill reaction by Wessels JSC (1956)
10.1093/oxfordjournals.pcp.a075684

Dates

Type	When
Created	23 years ago (Aug. 25, 2002, 12:51 a.m.)
Deposited	1 year, 10 months ago (Oct. 16, 2023, 9:54 p.m.)
Indexed	2 months, 1 week ago (June 26, 2025, 4:48 a.m.)
Issued	23 years, 6 months ago (Feb. 6, 2002)
Published	23 years, 6 months ago (Feb. 6, 2002)
Published Online	23 years, 6 months ago (Feb. 6, 2002)
Published Print	23 years, 4 months ago (April 5, 2002)

Funders 2

NATO Linkage Grant
Grant Agency of the Czech Republic 10.13039/501100001824 Grantová Agentura České Republiky
Region: Europe
pri (Trusts, charities, foundations (both public and private))
Labels9
1. Grant Agency of the Czech Republic
2. Czech Science Foundation
3. Grantová agentura ČR
4. Grantové agentury
5. GrantovaAgentura
6. GAČR
7. GACR
8. GA ČR
9. GA CR
Awards4
1. BE (95)1745
2. 522/00/1274
3. EUContractNo.QLK3-CT-2001-01629
4. HTECH.LG950980 EV5V/CT(94)0358

BibTeX

@article{Kobl_ek_2002, title={A biosensor for the detection of triazine and phenylurea herbicides designed using Photosystem II coupled to a screen‐printed electrode}, volume={78}, ISSN={1097-0290}, url={http://dx.doi.org/10.1002/bit.10190}, DOI={10.1002/bit.10190}, number={1}, journal={Biotechnology and Bioengineering}, publisher={Wiley}, author={Koblížek, Michal and Malý, Jan and Masojídek, Jiří and Komenda, Josef and Kučera, Tomáš and Giardi, Maria T. and Mattoo, Autar K. and Pilloton, Roberto}, year={2002}, month=feb, pages={110–116} }

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