DOI: 10.1002/bip.360250613. SANS studies of concentrated protein solutions. I. Bovine serum albumin

SANS studies of concentrated protein solutions. I. Bovine serum albumin

10.1002/bip.360250613

Crossref journal-article

Wiley

Biopolymers (311)

Abstract

AbstractSmall‐angle neutron scattering (SANS) was used to examine concentrated bovine serum albumin solutions of up to 20% protein w/v. At higher protein concentrations, scattering data show distinct features that can be ascribed to strong intermolecular interactions. Differential scattering cross‐sections are fitted to a theoretical model of interparticle potential consisting of a hard core plus an exponentially decaying “tail.” For moderate ionic strength (0.03M K Acetate, pH 5.9), the intermolecular interaction agrees with the double‐layer repulsive part of the well‐known DLVO (Derjaguin, Landau, Verwey, Overbeek) theory for interacting colloidal particles. We thus demonstrate that it is possible to determine size parameters and the surface charge of protein molecules in dense solutions. At high salt concentrations (≥0.2M NaCl) data can be fitted by the same potential model, although interpretation in terms of DLVO theory is not possible. Even in this case, however, “effective” molecular size and potential parameters can be determined.

Bibliography

Nossal, R., Glinka, C. J., & Chen, S. âH. (1986). SANS studies of concentrated protein solutions. I. Bovine serum albumin. Biopolymers, 25(6), 1157â1175. Portico.

Authors 3

R. Nossal (first)
C. J. Glinka (additional)
S.‐H. Chen (additional)

References 37 Referenced 35

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Dates

Type	When
Created	20 years, 8 months ago (Dec. 29, 2004, 9:01 p.m.)
Deposited	1 year, 10 months ago (Oct. 21, 2023, 8:40 p.m.)
Indexed	1 year ago (Aug. 30, 2024, 8:22 a.m.)
Issued	39 years, 2 months ago (June 1, 1986)
Published	39 years, 2 months ago (June 1, 1986)
Published Online	21 years, 6 months ago (Feb. 1, 2004)
Published Print	39 years, 2 months ago (June 1, 1986)

Funders 0

None

BibTeX

@article{Nossal_1986, title={SANS studies of concentrated protein solutions. I. Bovine serum albumin}, volume={25}, ISSN={1097-0282}, url={http://dx.doi.org/10.1002/bip.360250613}, DOI={10.1002/bip.360250613}, number={6}, journal={Biopolymers}, publisher={Wiley}, author={Nossal, R. and Glinka, C. J. and Chen, S.‐H.}, year={1986}, month=jun, pages={1157–1175} }

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