DOI: 10.1002/bip.360100913. The infrared spectrum and water binding of collagen as a function of relative humidity

The infrared spectrum and water binding of collagen as a function of relative humidity

10.1002/bip.360100913

Crossref journal-article

Wiley

Biopolymers (311)

Abstract

AbstractThe infrared spectra of undenatured bovine tendon collagen were investigated at 25°C and relative humidities from 0 to 95%. Suitable samples were prepared by forcing frozen suspensions of the material in distilled water through a stainless steel capillary. The samples were investigated by electron microscopy before and after the spectra were obtained to ascertain that no denaturation had taken place while the sample was exposed to infrared radiation. Temperature controlled absorption cells were constructed which permitted the passing of air with a known water content over the sample film. Gradual changes were observed in the frequencies and intensities of characteristic amide bands over the relative humidity range of 0 to 75%. These changes are particularly pronounced for the amide II band, associated with bending motions of peptide NH bonds. They lead to the conclusion that water molecules are gradually attached to peptide NH bonds within the triple helix over a wide range of relative humidity. Changes in CH deformation bands suggest that CH to O hydrogen bonding does occur and that it is more pronounced in collagen exposed to high relative humidity.

Bibliography

Susi, H., Ard, J. S., & Carroll, R. J. (1971). The infrared spectrum and water binding of collagen as a function of relative humidity. Biopolymers, 10(9), 1597â1604. Portico.

Authors 3

H. Susi (first)
J. S. Ard (additional)
R. J. Carroll (additional)

References 21 Referenced 98

10.1063/1.1730999
10.1021/ja01464a042
10.1016/S0022-2836(62)80107-7
10.1016/S0021-9258(18)99381-6
10.1016/S0021-9258(18)99382-8
{'key': 'e_1_2_1_7_2', 'first-page': '43', 'volume-title': 'Treatise on Collagen', 'author': 'Ramachandran G.', 'year': '1967'} / Treatise on Collagen by Ramachandran G. (1967)
10.1002/bip.1968.360061109
10.1021/ja00887a004
10.1021/ja00887a005
10.1063/1.1740293 / J. Chem. Phys. by Badger R. M. (1954)
10.1063/1.1740486 / J. Chem. Phys. by Sutherland G. B. B. M. (1954)
10.1039/df9582500173
{'key': 'e_1_2_1_13_2', 'first-page': '408', 'volume': '145', 'author': 'Hue A.', 'year': '1968', 'journal-title': 'Biochim. Biophys. Acta'} / Biochim. Biophys. Acta by Hue A. (1968)
10.1088/0508-3443/6/6/302
10.1021/ie50136a022
10.1016/0022-2852(60)90076-X
10.1063/1.1744547
{'key': 'e_1_2_1_18_2', 'volume-title': 'The Infrared Spectra of Complex Molecules', 'author': 'Bellamy L. J.', 'year': '1958'} / The Infrared Spectra of Complex Molecules by Bellamy L. J. (1958)
{'key': 'e_1_2_1_19_2', 'volume-title': 'The Hydrogen Bond', 'author': 'Pimentel G. C.', 'year': '1960'} / The Hydrogen Bond by Pimentel G. C. (1960)
10.1063/1.1698966
10.6028/jres.044.004

Dates

Type	When
Created	20 years, 2 months ago (May 27, 2005, 12:02 p.m.)
Deposited	1 year, 10 months ago (Oct. 19, 2023, 9:53 a.m.)
Indexed	3 months ago (May 19, 2025, 4:05 a.m.)
Issued	53 years, 11 months ago (Sept. 1, 1971)
Published	53 years, 11 months ago (Sept. 1, 1971)
Published Online	21 years, 6 months ago (Feb. 1, 2004)
Published Print	53 years, 11 months ago (Sept. 1, 1971)

Funders 0

None

BibTeX

@article{Susi_1971, title={The infrared spectrum and water binding of collagen as a function of relative humidity}, volume={10}, ISSN={1097-0282}, url={http://dx.doi.org/10.1002/bip.360100913}, DOI={10.1002/bip.360100913}, number={9}, journal={Biopolymers}, publisher={Wiley}, author={Susi, H. and Ard, J. S. and Carroll, R. J.}, year={1971}, month=sep, pages={1597–1604} }

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